HCV Genotyping

Inkcazelo emfutshane:

Le khithi isetyenziselwa ukufumanisa i-genotyping ye-virus ye-hepatitis C (i-HCV) i-subtypes ye-1b, i-2a, i-3a, i-3b kunye ne-6a kwi-serum yeklinikhi / iisampuli ze-plasma ze-virus ye-hepatitis C (HCV). Inceda ekuxilongeni nasekunyangweni kwezigulane ze-HCV.

Thumela i-imeyile kuthi

Iinkcukacha zeMveliso

Iithegi zeMveliso

Igama lemveliso

HWTS-HP004-HCV Genotyping Kit yokuFumana (Fluorescence PCR)

I-Epidemiology

Intsholongwane ye-Hepatitis C (HCV) yeyosapho lwe-flaviviridae, kwaye i-genome yayo yi-RNA enye echanekileyo, eguqulwa ngokulula. Intsholongwane ikhona kwi-hepatocytes, i-serum leukocytes kunye ne-plasma yabantu abosulelekileyo. Ufuzo lwe-HCV luchaphazeleka ekuguqukeni kwaye lunokwahlulwa ubuncinane kwii-genotypes ezi-6 kunye nee-subtypes ezininzi. I-HCV genotypes ezahlukeneyo zisebenzisa iirejimeni zonyango ze-DAA ezahlukeneyo kunye nezifundo zonyango. Ngoko ke, phambi kokuba izigulana zinyangwe ngonyango lwe-DAA lwe-antiviral, i-HCV genotype kufuneka ibonwe, kwaye nakwizigulana ezinohlobo loku-1, kuyimfuneko ukwahlula ukuba luhlobo 1a okanye uhlobo 1b.

Umjelo

FAM	Uhlobo 1b, Uhlobo 2a
ROX	Uhlobo 6a, Uhlobo lwe-3a
VIC/HEX	Ulawulo lwangaphakathi, Uhlobo lwe-3b

Iiparamitha zobuGcisa

Ugcino	≤-18℃ ebumnyameni
Beka ubomi kwishelufa	Iinyanga ezili-9
Uhlobo loMfanekiso	ISerum, iPlasma
Ct	≤36
CV	≤5.0％
LoD	200 IU/mL
Ukuchaza ngokuthe ngqo	Sebenzisa le khithi ukubona ezinye iintsholongwane okanye iisampuli zebhaktheriya ezifana: i-cytomegalovirus yabantu, i-Epstein-Barr virus, i-virus ye-immunodeficiency virus, i-virus ye-hepatitis B, i-virus ye-hepatitis A, i-syphilis, i-herpes virus ye-herpes yohlobo lwe-6, i-herpes simplex virus type 1, i-simplex Herpes virus type 2, i-influenza A virus, iziphumo ze-Propioniphylocan, i-acne zonke zinegative.
Izixhobo Ezisebenzayo	Iyakwazi ukuhambelana nezixhobo zePCR zefluorescent kwimarike. Iinkqubo zePCR ze-ABI 7500 ABI 7500 Fast Real-Time PCR Systems SLAN-96P Iinkqubo zePCR zeXesha langempela I-QuantStudio®5 Iinkqubo zePCR zeXesha langempela Iinkqubo zePCR zeLightCycler®480 LineGene 9600 Plus Real-Time PCR Ukufunyanwa Systems MA-6000 Real-Time Quantitative Thermal Cycler I-BioRad CFX96 Inkqubo yePCR yexesha lokwenene BioRad CFX Opus 96 Real-Time PCR System