Uhlalutyo lwe-HCV
Igama lemveliso
Ikhithi yokuFumanisa iGenotyping ye-HWTS-HP004-HCV (i-Fluorescence PCR)
I-Epidemiology
Intsholongwane yeHepatitis C (HCV) yeyosapho lwe-flaviviridae, kwaye i-genome yayo yi-RNA enye entle, etshintsha ngokulula. Le ntsholongwane ikhona kwi-hepatocytes, kwi-serum leukocytes nakwi-plasma yabantu abanentsholongwane. Iijini ze-HCV zichaphazeleka kuguquko kwaye zinokwahlulwa zibe zii-genotypes ezi-6 ubuncinane kunye nee-subtypes ezininzi. Ii-genotypes ezahlukeneyo ze-HCV zisebenzisa iindlela ezahlukeneyo zonyango lwe-DAA kunye nezifundo zonyango. Ke ngoko, ngaphambi kokuba izigulana zinyangwe ngonyango lwe-DAA antiviral, i-genotype ye-HCV kufuneka ifunyanwe, kwaye nakwizigulana ezinesifo sohlobo loku-1, kuyimfuneko ukwahlula ukuba luhlobo lwe-1a okanye uhlobo lwe-1b.
Itshaneli
| I-FAM | Uhlobo 1b, Uhlobo 2a |
| I-ROX | Uhlobo 6a, Uhlobo 3a |
| I-VIC/HEX | Ulawulo lwangaphakathi, Uhlobo 3b |
Iiparameters zobugcisa
| Indawo yokugcina izinto | ≤-18℃ Ebumnyameni |
| Beka ubomi kwishelufa | Iinyanga ezi-9 |
| Uhlobo lweSibonelelo | I-Serum, iPlasma |
| Ct | ≤36 |
| CV | ≤5.0％ |
| I-LoD | 200 IU/mL |
| Ukuchaneka | Sebenzisa le khithi ukubona ezinye iintsholongwane okanye iisampuli zeentsholongwane ezifana: i-human cytomegalovirus, i-Epstein-Barr virus, i-human immunodeficiency virus, i-hepatitis B virus, i-hepatitis A virus, i-syphilis, i-human herpes virus type 6, i-herpes simplex virus type 1, i-simplex i-Herpes virus type 2, i-influenza A virus, i-Propionibacterium acnes, i-Staphylococcus aureus, i-Candida albicans, njl. Zonke iziphumo azilunganga. |
| Izixhobo Ezisebenzayo | Ingalingana nezixhobo zePCR ezikhanyayo ezithengiswayo. Iinkqubo ze-ABI 7500 ze-Real-Time PCR Iinkqubo ze-PCR ze-ABI 7500 ezikhawulezayo zeXesha langempela Iinkqubo ze-PCR ze-SLAN-96P zeXesha langempela Iinkqubo ze-PCR zeQuantStudio®5 zeXesha langempela Iinkqubo ze-PCR zeLightCycler®480 zeXesha langempela Iinkqubo zokuFumanisa i-LineGene 9600 Plus zeXesha langempela ze-PCR I-MA-6000 Real-Time Quantitative Thermal Cycler Inkqubo ye-PCR ye-BioRad CFX96 yeXesha langempela Inkqubo ye-PCR ye-BioRad CFX Opus 96 yeXesha langempela |
Ukuhamba komsebenzi
