Uhlalutyo lwe-Hepatitis B Virus
Igama lemveliso
Ikhithi yokuHlola iGenotyping yeVirus ye-HWTS-HP002-Hepatitis B (i-Fluorescent PCR)
I-Epidemiology
I-Epidemiology
Itshaneli
| ItshaneliIgama | I-Reaction Buffer 1 | I-Reaction Buffer 2 |
| I-FAM | I-HBV-C | I-HBV-D |
| I-VIC/HEX | I-HBV-B | Ulawulo lwangaphakathi |
Iiparameters zobugcisa
| Indawo yokugcina izinto | ≤-18℃ Ebumnyameni |
| Beka ubomi kwishelufa | Iinyanga ezili-12 |
| Uhlobo lweSibonelelo | I-Serum, iPlasma |
| Ct | ≤38 |
| CV | ≤5.0％ |
| I-LoD | 1 × 102IU/mL |
| Ukuchaneka | Akukho ntshukumo idibeneyo nentsholongwane ye-hepatitis C, i-human cytomegalovirus, i-Epstein-Barr virus, i-human immunodeficiency virus, i-hepatitis A virus, i-syphilis, i-herpes virus, i-influenza A virus, i-propionibacterium acnes (PA), njl. |
| Izixhobo Ezisebenzayo | Ingalingana nezixhobo zePCR ezikhanyayo ezithengiswayo. Iinkqubo ze-ABI 7500 ze-Real-Time PCR Iinkqubo ze-PCR ze-ABI 7500 ezikhawulezayo zeXesha langempela Iinkqubo ze-PCR ze-SLAN-96P zeXesha langempela Iinkqubo ze-PCR zeQuantStudio®5 zeXesha langempela Iinkqubo ze-PCR zeLightCycler®480 zeXesha langempela Iinkqubo zokuFumanisa i-LineGene 9600 Plus zeXesha langempela ze-PCR I-MA-6000 Real-Time Quantitative Thermal Cycler Inkqubo ye-PCR ye-BioRad CFX96 yeXesha langempela Inkqubo ye-PCR ye-BioRad CFX Opus 96 yeXesha langempela |
I-reagent yokukhupha ecetyiswayo: I-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) engasetyenziswa kunye ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ011)) yiJiangsu Macro & Micro-Test Med-Tech Co., Ltd. Ukukhupha kufuneka kwenziwe ngokwemiyalelo, umthamo wesampulu okhutshiweyo ngu200μL, kwaye umthamo we-elution ocetyiswayo ngu80μL.
I-reagent yokukhupha ecetyiswayo: I-Nucleic Acid Extraction okanye i-Purification Reagent (YDP315). Ukukhupha kufuneka kwenziwe ngokuhambelana nemiyalelo. Umthamo wesampulu okhutshiweyo yi-200µL, kwaye umthamo wokucoca ocetyiswayo yi-100µL.
