Intsholongwane yeHepatitis B
Igama lemveliso
Ikhithi yokuFumanisa iNucleic Acid yeHWTS-HP001-Hepatitis B Virus (iFluorescence PCR)
I-Epidemiology
I-Hepatitis B sisifo esosulelayo esinezilonda zesibindi kunye namalungu amaninzi abangelwa yintsholongwane ye-hepatitis B (HBV). Uninzi lwabantu lufumana iimpawu ezifana nokudinwa kakhulu, ukuphelelwa ngumdla wokutya, amalungu asezantsi okanye ukudumba komzimba wonke, i-hepatomegaly, njl. I-5% yabantu abadala kunye ne-95% yabantwana abagulayo abasuleleke ngoomama babo abakwazi ukuyicoca intsholongwane ye-HBV ngokufanelekileyo kwisifo esingapheliyo kwaye baqhubekele kwi-cirrhosis yesibindi okanye i-carcinoma yeseli yesibindi ephambili..
Itshaneli
| I-FAM | I-HBV-DNA |
| I-VIC (HEX) | Isalathiso sangaphakathi |
Iiparameters zobugcisa
| Indawo yokugcina izinto | ≤-18℃ Ebumnyameni |
| Beka ubomi kwishelufa | Iinyanga ezili-12 |
| Uhlobo lweSibonelelo | Igazi lemithambo yegazi |
| Ct | ≤33 |
| CV | ≤5.0％ |
| I-LoD | 25IU/mL |
| Ukuchaneka | Akukho kudibana kwe-Cytomegalovirus, i-EB virus, i-HIV, i-HAV, i-Syphilis, i-Human Herpesvirus-6, i-HSV-1/2, i-Influenza A, i-Propionibacterium Acnes, i-Staphylococcus Aureus kunye ne-Candida albican. |
| Izixhobo Ezisebenzayo | Ingalingana nezixhobo zePCR ezikhanyayo ezithengiswayo. Iinkqubo ze-ABI 7500 ze-Real-Time PCR Iinkqubo ze-PCR ze-ABI 7500 ezikhawulezayo zeXesha langempela Iinkqubo ze-PCR ze-SLAN-96P zeXesha langempela Iinkqubo ze-PCR zeQuantStudio®5 zeXesha langempela Iinkqubo ze-PCR zeLightCycler®480 zeXesha langempela Iinkqubo zokuFumanisa i-LineGene 9600 Plus zeXesha langempela ze-PCR I-MA-6000 Real-Time Quantitative Thermal Cycler Inkqubo ye-PCR ye-BioRad CFX96 yeXesha langempela Inkqubo ye-PCR ye-BioRad CFX Opus 96 yeXesha langempela |
Ii-reagents zokukhupha ezicetyiswayo: Macro kunye neMicro-TestIntsholongwaneI-DNA/RNA Kit (HWTS-3017) (engasetyenziswa ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ011)) yiJiangsu Macro & Micro-Test Med-Tech Co., Ltd.. Ukukhupha kufuneka kuqalwe ngokwe-IFU ye-reagent yokukhupha. Umthamo wesampulu okhutshiweyo yi-200µL kwaye umthamo wokucoca ocetyiswayo yi-80 μL.
Ii-reagents zokukhupha ezicetyiswayo: Ii-Nucleic Acid Extraction okanye ii-Purification Reagents (YDP315). Ukukhupha kufuneka kuqalwe ngokuhambelana ne-IFU. Umthamo wesampulu okhutshiweyo yi-200µL kwaye umthamo wokucoca ocetyiswayo yi-100 μL.
