Hepatitis B Virus RNA
Igama lemveliso
HWTS-HP007 Hepatitis B Virus RNA Nucleic Acid Detection Kit (Fluorescence PCR)
I-Epidemiology
Qukufumanisa okungalindelekanga kwamanqanaba e-HBV-RNA kwi-serum kunokubeka iliso ngcono izinga le-HBV cccDNA kwi-hepatocytes, kwaye kwangaxeshanye ugweme ukubangela umonakalo omkhulu kwizigulane.Kubaluleke kakhulu kuxilongo oluncedisayo losulelo lwentsholongwane ye-hepatitis B (HBV) kunye nokujongwa ngempumelelo kunye noqikelelo lokurhoxa kweziyobisi lwe-NAs yonyango lwe-antiviral kwizigulana ze-CHB.
Umjelo
| FAM | HBV-RNA |
| ROX | Ulawulo lwangaphakathi |
IiParameters zobuGcisa
| Ugcino | ≤-18℃ |
| Beka ubomi kwishelufa | Iinyanga ezili-9 |
| Uhlobo loMfanekiso | serum |
| Tt | ≤42 |
| CV | ≤5.0% |
| LoD | Iikopi ezili-100/mL |
| Ukuchaza ngokuthe ngqo | Iziphumo zovavanyo lokuphazamiseka zibonisa ukuba akukho ziphumo ekumiselweni kwe-HBV RNA xa ukuxinwa kwe-bilirubin kwi-serum kungekho ngaphezu kwe-168.2μmol/mL, ukuxinwa kwe-hemoglobin eveliswa yi-hemolysis akukho ngaphezu kwe-130g/L, kwaye ukuxinwa kwe-lipids yegazi akukho ngaphezu kwe-65mmol / mL;akukho miphumo ekumiselweni kwe-HBV RNA xa i-concentration ye-IgG iyonke kwi-serum ayikho ngaphezu kwe-5mg / mL.Iziphumo zovavanyo lwe-cross-reactivity zibonisa ukuba akukho zimpendulo phakathi kwale khithi kunye nezinye iintsholongwane okanye ibhaktiriya efunyenwe yi-nucleic acid kwiisampuli zegazi (intsholongwane ye-hepatitis C, i-cytomegalovirus yabantu, i-epstein-barr virus, i-human immunodeficiency virus, intsholongwane ye-hepatitis A, i-syphilis. , uhlobo lwesi-6 lwentsholongwane ye-herpes, i-herpes simplex virus type 1, i-herpes simplex virus type 2, i-influenza A virus, i-propionibacterium acnes, i-staphylococcus aureus, i-candida albicans) kunye ne-genomes yabantu;emva kokuba izigulane zithathe i-lamivudine, i-telbivudine, i-adefovir dipivoxil kunye ne-entecavir, xa umthamo wentsholongwane we-hepatitis B emzimbeni ungaphezulu kwe-LoD encinci yekiti, ikiti isenokuthi ibonwe ngokufanelekileyo. |
| Izixhobo Ezisebenzayo | Isetyenziselwe i-Biosystems ye-7500 ye-Real-Time PCR SystemIinkqubo ze-Biosystems eziSetyenzisiweyo 7500 Iinkqubo zePCR ezikhawulezayo SLAN-96P Iinkqubo zePCR zeXesha lokwenyani (Hongshi Medical Technology Co., Ltd.) |
Ukuhamba komsebenzi
Ukhetho loku-1.
I-Viral DNA/RNA Isolation Kit (NA007-2) yiGenMag Biotechnology Co., Ltd. Amanyathelo athile okukhutshwa nceda ulandele ngokungqongqo incwadana yomyalelo.
IMacro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (enokuthi isetyenziswe kunye neMacro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) yi-Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Ukukhutshwa kufuneka kuqhutywe ngokuhambelana nencwadana yomyalelo, umthamo wesampulu ekhutshiweyo yi-200μL, kunye nomthamo we-elution ocetyiswayo. 80μL.
