I-HPV16 kunye ne-HPV18
Igama lemveliso
I-HWTS-CC001-Ikhithi yokuFumanisa i-Nucleic Acid ye-HPV16 kunye ne-HPV18 (i-Fluorescence PCR)
I-Epidemiology
Umhlaza womlomo wesibeleko yenye yezona zifo zixhaphakileyo kwindlela yokuzala yabasetyhini. Kuye kwabonakala ukuba usulelo oluqhubekayo lwe-HPV kunye nosulelo oluninzi zezinye zezizathu eziphambili zomhlaza womlomo wesibeleko. Okwangoku kusekho ukunqongophala konyango olusebenzayo olwamkelweyo ngokubanzi lomhlaza womlomo wesibeleko olubangelwa yi-HPV. Ke ngoko, ukufunyaniswa kwangoko kunye nokuthintela usulelo lomlomo wesibeleko olubangelwa yi-HPV zizitshixo zokuthintela umhlaza womlomo wesibeleko. Ukusekwa kovavanyo olulula, olucacileyo nolukhawulezileyo lokuxilonga iintsholongwane kubaluleke kakhulu ekuxilongweni komhlaza womlomo wesibeleko ngokwezonyango.
Itshaneli
| Itshaneli | Uhlobo |
| I-FAM | I-HPV18 |
| I-VIC/HEX | I-HPV16 |
| CY5 | Ulawulo lwangaphakathi |
Iiparameters zobugcisa
| Indawo yokugcina izinto | ≤-18℃ |
| Beka ubomi kwishelufa | Iinyanga ezili-12 |
| Uhlobo lweSibonelelo | iseli ekhutshiweyo yomlomo wesibeleko |
| Ct | ≤28 |
| CV | ≤10.0% |
| I-LoD | Iikopi ezingama-500/mL |
| Ukuchaneka | Xa usebenzisa ikhithi ukuvavanya iisampuli ezingachazwanga ezinokudibana neethagethi zayo, zonke iziphumo azibi, kuquka i-Ureaplasma urealyticum, i-Chlamydia trachomatis, i-Candida albicans, i-Neisseria gonorrhoeae, i-Trichomonas vaginalis, i-Mildew, i-Gardnerella kunye nezinye iintlobo ze-HPV ezingagutyungelwanga yikhithi. |
| Izixhobo Ezisebenzayo | I-SLAN®-96P Iinkqubo zePCR zeXesha langempelaIinkqubo ze-PCR ze-Applied Biosystems 7500 Real-Time I-QuantStudio®Iinkqubo ezi-5 ze-PCR zeXesha langempela I-LightCycler®Inkqubo ye-PCR yeXesha langempela ye-480 Inkqubo yokuFumanisa i-LineGene 9600 Plus yeXesha langempela le-PCR (i-FQD-96A, iteknoloji ye-Hangzhou Bioer) I-MA-6000 Real-Time Quantitative Thermal Cycler. |
Ukuhamba komsebenzi
Ukhetho1.
I-Macro kunye ne-Micro-Test Sample Release Reagent (HWTS-3005-8), kufuneka ikhutshwe ngokwemiyalelo.
Ukhetho2.
I-Macro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) kunye ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006B, HWTS-3006C), kufuneka ikhutshwe ngokwemiyalelo. Umthamo wesampulu okhutshiweyo yi-200μL, kwaye umthamo wokucoca ocetyiswayo yi-80µL.
Ukhetho 3.
I-QIAamp DNA Mini Kit (51304) eyenziwe yi-QIAGEN okanye i-TIANamp Virus DNA/RNA Kit (YDP315) eyenziwe yi-Tiangen Biotech (Beijing) Co., Ltd., kufuneka ikhutshwe ngokuhambelana nemiyalelo. Umthamo wesampulu okhutshiweyo yi-200μL, kwaye umthamo wokucoca ocetyiswayo yi-80μL.
