I-HPV16 kunye ne-HPV18
Igama lemveliso
HWTS-CC001-I-HPV16 kunye ne-HPV18 Nucleic Acid Detection Kit (Fluorescence PCR)
I-Epidemiology
Umhlaza womlomo wesibeleko lelinye lawona mathumba ayingozi axhaphakileyo kwindlela yokuzala yabasetyhini.Kuye kwaboniswa ukuba usulelo lwe-HPV oluzingisileyo kunye nosulelo oluninzi ngomnye woonobangela abaphambili bomhlaza womlomo wesibeleko.Okwangoku kusekho ukunqongophala konyango olwamkelekileyo jikelele lomhlaza womlomo wesibeleko obangelwa yi-HPV.Ke ngoko, ukufunyaniswa kwangoko kunye nokuthintela usulelo lomlomo wesibeleko olubangelwa yi-HPV zizitshixo zokuthintela umhlaza womlomo wesibeleko.Ukusekwa kweemvavanyo ezilula, ezicacileyo kunye nezikhawulezayo zokuxilonga iintsholongwane zibaluleke kakhulu kuxilongo lwezonyango lomhlaza womlomo wesibeleko.
Umjelo
| Umjelo | Uhlobo |
| FAM | HPV18 |
| VIC/HEX | HPV16 |
| CY5 | Ulawulo lwangaphakathi |
IiParameters zobuGcisa
| Ugcino | ≤-18℃ |
| Beka ubomi kwishelufa | Iinyanga ezili-12 |
| Uhlobo loMfanekiso | iseli yomlomo wesibeleko exfoliated |
| Ct | ≤28 |
| CV | ≤10.0% |
| LoD | Iikopi ezingama-500/mL |
| Ukuchaza ngokuthe ngqo | Xa usebenzisa ikiti ukuvavanya iisampulu ezingezizo ezinokuthi zisabele kwiithagethi zayo, zonke iziphumo zibi, kubandakanywa i-Ureaplasma urealyticum, Chlamydia trachomatis, Candida albicans, Neisseria gonorrhoeae, Trichomonas vaginalis, Mildew, Gardnerella kunye nezinye iintlobo zeHPV ezingagqunywanga yi. ikhithi. |
| Izixhobo Ezisebenzayo | SLAN®-96P Iinkqubo zePCR zeXesha langempelaIinkqubo ze-Biosystems ezisetyenzisiweyo 7500 Iinkqubo zePCR zeXesha langempela QuantStudio®IiNkqubo ze-PCR ze-5 zeXesha langempela I-LightCycler®Inkqubo ye-PCR ye-480 yexesha langempela I-LineGene 9600 Plus ye-Real-Time PCR Detection System (FQD-96A, iteknoloji ye-Hangzhou Bioer) MA-6000 Real-Time Quantitative Thermal Cycler. |
Ukuhamba komsebenzi
Ukhetho1.
I-Macro & Micro-Test Release Sample Release Reagent (HWTS-3005-8), kufuneka ikhutshwe ngokwemiyalelo.
Ukhetho2.
IMacro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) kunye neMacro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006B, HWTS-3006C), kufuneka ikhutshwe ngokwemiyalelo.Umthamo wesampulu ekhutshiweyo yi-200μL, kwaye umthamo we-elution ocetyiswayo yi-80µL.
Ukhetho3.
I-QIAamp DNA Mini Kit (51304) eyenziwe yi-QIAGEN okanye i-TIANamp Virus DNA/RNA Kit (YDP315) eyenziwe yi-Tiangen Biotech (Beijing) Co., Ltd., kufuneka ikhutshwe ngokuhambelana nemiyalelo.Umthamo wesampulu ekhutshiweyo yi-200μL, kwaye umthamo we-elution ocetyiswayo yi-80µL.
