I-Influenza A Virus Nucleic Acid

Umfanekiso obonisiweyo we-Influenza A Virus Nucleic Acid

Inkcazo emfutshane:

Le khithi isetyenziselwa ukufumanisa i-Influenza A virus nucleic acid kwiiswabs zomphimbo womntu kwi-vitro.

Iinkcukacha zeMveliso

Iithegi zeMveliso

Igama lemveliso

Ikhithi yokuFumanisa i-HWTS-RT049A-Nucleic Acid esekwe kwi-Enzymatic Probe Isothermal Amplification (EPIA) yentsholongwane ye-Influenza A

Ikhithi yokuFumana i-Influenza A Virus Nucleic Acid Detection Kit ye-HWTS-RT044-eyomileyo ngokuqandisa (i-Isothermal Amplification)

Isatifikethi

I-Epidemiology

Intsholongwane yomkhuhlane luhlobo olumele i-Orthomyxoviridae. Yintsholongwane ebangela ingozi enkulu kwimpilo yabantu. Ingosulela umninimzi kakhulu. Ubhubhane wexesha lonyaka uchaphazela abantu abamalunga nezigidi ezingama-600 kwihlabathi liphela kwaye ubangela ukufa kwabantu abangama-250,000 ukuya kuma-500,000, apho intsholongwane yomkhuhlane A yeyona nto iphambili yosulelo nokufa. Intsholongwane yomkhuhlane A (intsholongwane yomkhuhlane A) yi-RNA enemigca enye enemigca engalunganga. Ngokwe-hemagglutinin yayo engaphezulu (HA) kunye ne-neuraminidase (NA), i-HA inokwahlulwa ibe ziintlobo ngeentlobo ezili-16, i-NA yahlulwe yaba ziintlobo ngeentlobo ezili-9. Phakathi kweentsholongwane zomkhuhlane A, iintlobo ngeentlobo zeentsholongwane zomkhuhlane ezinokosulela abantu ngokuthe ngqo zezi: A H1N1, H3N2, H5N1, H7N1, H7N2, H7N3, H7N7, H7N9, H9N2 kunye ne-H10N8. Phakathi kwazo, iintlobo ze-H1, H3, H5, kunye ne-H7 zibangela izifo ezininzi, kwaye i-H1N1, H3N2, H5N7, kunye ne-H7N9 zifaneleke kakhulu ukuqwalaselwa. Ubunzima be-influenza A virus bunokutshintshatshintsha, kwaye kulula ukwenza iintlobo ezintsha, nto leyo ebangela ubhubhane wehlabathi. Ukususela ngoMatshi 2009, iMexico, i-United States kunye namanye amazwe aqalise ubhubhane olutsha lwe-influenza H1N1 uhlobo A, kwaye luye lwasasazeka ngokukhawuleza kwihlabathi liphela. I-Influenza A virus inokudluliselwa ngeendlela ezahlukeneyo ezifana nendlela yokugaya ukutya, indlela yokuphefumla, umonakalo wesikhumba, kunye neliso kunye ne-conjunctiva. Iimpawu emva kosulelo ikakhulu ngumkhuhlane ophezulu, ukukhwehlela, impumlo egobhozayo, i-myalgia, njl.njl., uninzi lwazo luhamba ne-pneumonia enzima. Intliziyo, izintso kunye nezinye izitho zomzimba zabantu abasuleleke kakhulu zikhokelela ekufeni, kwaye izinga lokufa liphezulu. Ke ngoko, indlela elula, echanekileyo nekhawulezayo yokuxilonga intsholongwane ye-influenza A ifuneka ngokukhawuleza kwiklinikhi ukuze kubonelelwe ngesikhokelo samayeza eklinikhi kunye nokuxilongwa.

Itshaneli

I-FAM	I-IVA nucleic acid
I-ROX	Ulawulo lwangaphakathi

Iiparameters zobugcisa

Indawo yokugcina izinto	Ulwelo: ≤-18℃ Ebumnyameni; I-Lyophilized: ≤30℃ Ebumnyameni
Beka ubomi kwishelufa	Ulwelo: Iinyanga ezili-9; I-Lyophilized: Iinyanga ezili-12
Uhlobo lweSibonelelo	Iiswabhu zomqala ezisandula ukuqokelelwa
CV	≤10.0%
Tt	≤40
I-LoD	1000Cii-opies/mL
Ukuchaneka	Tapha akukho kudibana kwe-InfluenzaB, I-Staphylococcus aureus, I-Streptococcus (kuquka i-Streptococcus pneumoniae), I-Adenovirus, I-Mycoplasma pneumoniae, I-Respiratory Syncytial Virus, I-Mycobacterium tuberculosis, I-Maasles, I-Haemophilus influenzae, I-Rhinovirus, I-Coronavirus, I-Enteric Virus, i-swab yomntu ophilileyo.
Izixhobo Ezisebenzayo:	I-Applied Biosystems 7500 Real-Time PCR IinkquboIinkqubo ze-PCR ze-SLAN ® -96P zeXesha langempela Inkqubo ye-PCR yeLightCycler® 480 yeXesha langempela Inkqubo yokuFumanisa i-Fluorescence Isothermal ye-Easy Amp Real-time （HWTS1600）

Ukuhamba komsebenzi

Ukhetho 1.

I-reagent yokukhupha ecetyiswayo: I-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3001, HWTS-3004-32, HWTS-3004-48) kunye ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006).

Ukhetho 2.

I-reagent yokukhupha ecetyiswayo: I-Nucleic Acid Extraction okanye i-Purification Reagent (YDP302) yiTiangen Biotech (Beijing) Co.,Ltd.

Ngaphambili: Iintlobo ezili-19 ze-Respiratory Pathogen Nucleic Acid

Okulandelayo: I-Influenza B Virus Nucleic Acid

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