I-Influenza B Virus Nucleic Acid

Umfanekiso obonisiweyo we-Influenza B Virus Nucleic Acid

Inkcazo emfutshane:

Le khithi yenzelwe ukufumanisa i-in vitro qualitatively ye-Influenza B virus nucleic acid kwiisampuli ze-nasopharyngeal kunye ne-oropharyngeal swab.

Thumela i-imeyile kuthi

Iinkcukacha zeMveliso

Iithegi zeMveliso

Igama lemveliso

Ikhithi yokuFumanisa i-HWTS-RT127A-Influenza B Virus Nucleic Acid (Ukwandiswa kwe-Enzymatic Probe Isothermal)

Isatifikethi

I-Epidemiology

Intsholongwane yomkhuhlane, uhlobo olumele i-Orthomyxoviridae, yintsholongwane esongela impilo yabantu kakhulu kwaye inokosulela abantu abaninzi. Ubhubhane womkhuhlane wexesha lonyaka uhlasela abantu abamalunga nezigidi ezingama-600 kwihlabathi liphela kwaye ubangela ukufa kwabantu abangama-250,000 ukuya kuma-500,000 minyaka le, apho intsholongwane yomkhuhlane B yenye yezona zinto ziphambili.^[1]Intsholongwane ye-Influenza B, eyaziwa ngokuba yi-IVB, yi-RNA ene-strand negative ene-strand enye. Ngokwe-nucleotide sequence ye-antigenic characteristic HA1 region yayo, inokwahlulwa ibe zii-lineages ezimbini ezinkulu, iintlobo ezimeleyo yi-B/Yamagata/16/88 kunye ne-B/Victoria /2/87(5)^[2]. Intsholongwane ye-Influenza B ngokubanzi inamandla kakhulu kwi-host. Kufunyenwe ukuba i-IVB inokosulela abantu kunye neenja kuphela, kwaye ngokubanzi ayibangeli ubhubhane wehlabathi liphela, kodwa inokubangela ubhubhane wexesha lonyaka wengingqi.^[3]Intsholongwane ye-Influenza B inokudluliselwa ngeendlela ezahlukeneyo ezifana nendlela yokugaya ukutya, indlela yokuphefumla, umonakalo wolusu kunye ne-conjunctiva. Iimpawu ikakhulu ngumkhuhlane ophezulu, ukukhwehlela, impumlo egobhozayo, i-myalgia, njl. Uninzi lwazo luhamba ne-pneumonia enzima, uhlaselo lwentliziyo olubi. Kwiimeko ezinzima, ukungasebenzi kakuhle kwentliziyo, izintso kunye nezinye izitho zomzimba kukhokelela ekufeni, kwaye izinga lokufa liphezulu kakhulu.^[4]Ngoko ke, kukho imfuneko engxamisekileyo yendlela elula, echanekileyo nekhawulezayo yokubona intsholongwane yomkhuhlane B, enokubonelela ngesikhokelo samayeza ezonyango kunye nokuxilongwa.

Itshaneli

I-FAM	I-IVB nucleic acid
I-ROX	Ulawulo lwangaphakathi

Iiparameters zobugcisa

Indawo yokugcina izinto	Ulwelo: ≤-18℃ Ebumnyameni Ukwenziwa kwe-Lyophilization: ≤30℃ Ebumnyameni
Beka ubomi kwishelufa	Ulwelo: Iinyanga ezili-9 I-Lyophilization: iinyanga ezili-12
Uhlobo lweSibonelelo	Iisampuli ze-nasopharyngeal swab Iisampuli ze-Oropharyngeal swab
CV	≤10.0%
Tt	≤40
I-LoD	Ikopi e-1/µL
Ukuchaneka	akukho kudibana kwe-Influenza A, i-Staphylococcus aureus,I-Streptococcus (kuquka i-Streptococcus pneumoniae), i-Adenovirus, i-Mycoplasma pneumoniae, i-Respiratory Syncytial Virus, i-Mycobacterium tuberculosis, i-Maasles, i-Haemophilus influenzae, i-Rhinovirus, i-Coronavirus, i-Enteric Virus, i-swab yomntu ophilileyo.
Izixhobo Ezisebenzayo:	Iinkqubo ze-PCR ze-Applied Biosystems 7500 Real-Time Iinkqubo ze-PCR ze-SLAN ® -96P zeXesha langempela Inkqubo ye-PCR yeLightCycler® 480 yeXesha langempela Inkqubo yokuFumanisa i-Fluorescence Isothermal ye-Easy Amp Real-time （HWTS1600）

Ukuhamba komsebenzi

Ukhetho 1.

I-reagent yokukhupha ecetyiswayo: I-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3001, HWTS-3004-32, HWTS-3004-48) kunye ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006).

Ukhetho 2.

I-reagent yokukhupha ecetyiswayo: I-Nucleic Acid Extraction okanye i-Purification Reagent (YDP302) yiTiangen Biotech (Beijing) Co.,Ltd.

Ngaphambili: I-Influenza A Virus Nucleic Acid

Okulandelayo: I-Mycoplasma Pneumoniae Nucleic Acid

Bhala umyalezo wakho apha uze uwuthumele kuthi