I-Klebsiella Pneumoniae, i-Acinetobacter Baumannii kunye ne-Pseudomonas Aeruginosa kunye ne-Drug Resistance Genes (KPC, NDM, OXA48 kunye ne-IMP) Multiplex

I-Klebsiella Pneumoniae, i-Acinetobacter Baumannii kunye ne-Pseudomonas Aeruginosa kunye ne-Drug Resistance Genes (KPC, NDM, OXA48 kunye ne-IMP) i-Multiplex Umfanekiso okhoyo

Inkcazelo emfutshane:

Le khithi isetyenziselwa ukufumanisa umgangatho we-in vitro we-Klebsiella pneumoniae (KPN), i-Acinetobacter baumannii (Aba), i-Pseudomonas aeruginosa (PA) kunye neentlobo ezine zokumelana ne-carbapenem (eziquka i-KPC, i-NDM, i-OXA48 kunye ne-IMP) kwiisampuli zesikhohlela somntu, ukubonelela. isiseko sesikhokelo sokuxilongwa kweklinikhi, unyango kunye namayeza kwizigulana ezinosulelo olurhanelwa yintsholongwane.

Thumela i-imeyile kuthi

Iinkcukacha zeMveliso

Iithegi zeMveliso

Igama lemveliso

I-HWTS-RT109 Klebsiella Pneumoniae, i-Acinetobacter Baumannii kunye ne-Pseudomonas Aeruginosa kunye ne-Drug Resistance Genes (KPC, NDM, OXA48 kunye ne-IMP) i-Multiplex Detection Kit(Fluorescence PCR)

Isatifikethi

I-Epidemiology

I-Klebsiella pneumoniae yintsholongwane exhaphakileyo yonyango engenelelayo kwaye yenye yeebhaktheriya ezibalulekileyo ze-pathogenic ezibangela usulelo lwe-nosocomial.Xa ukuxhathisa komzimba kuncitshisiwe, iibhaktheriya zingena emiphungeni zisuka kwiphecana lokuphefumla, zibangela usulelo kwiindawo ezininzi zomzimba, kwaye ukusetyenziswa kwangoko kwamayeza okubulala iintsholongwane kusitshixo sokunyanga.^[1].

Eyona ndawo ixhaphakileyo yosulelo lwe-Acinetobacter baumannii yimiphunga, eyintsholongwane ebalulekileyo ye-Hospital gained pneumonia (HAP), ingakumbi i-Ventilator associated pneumonia (VAP).Ihlala ihamba kunye nolunye usulelo lwebhaktiriya kunye nokungunda, kunye neempawu zezinga eliphezulu lokugula kunye nezinga eliphezulu lokufa.

I-Pseudomonas aeruginosa yeyona nto ixhaphakileyo kwi-non-fermentative gram-negative bacilli kwi-clinical practice, kwaye iyintsholongwane ebalulekileyo engenelelayo yosulelo olufunyenwe esibhedlele, kunye neempawu zekholoni lula, ukuhluka okulula kunye nokuxhathisa iziyobisi ezininzi.

Umjelo

Igama	I-PCR-Mix 1	PCR-Mix 2
FAM Channel	Aba	IMP
VIC/HEX Channel	Ulawulo lwangaphakathi	I-KPC
Ijelo le-CY5	PA	NDM
Ijelo le-ROX	KPN	OXA48

IiParameters zobuGcisa

Ugcino	≤-18℃
Beka ubomi kwishelufa	Iinyanga ezili-12
Uhlobo loMfanekiso	Isikhohlela
Ct	≤36
CV	≤10.0%
LoD	1000 CFU/mL
Ukuchaza ngokuthe ngqo	a) Uvavanyo lwe-cross-reactivity lubonisa ukuba le khithi ayinakuphinda kusebenze kunye nezinye iintsholongwane zokuphefumla, ezifana neStreptococcus pneumoniae, Neisseria meningitidis, Staphylococcus aureus, Klebsiella oxytoca, Haemophilus influenzae, i-Acinetobacter jelly, Acinetobacter, Le Plenetobacter, Leiphila, i-Placimonas, i-Elenatobacter, i-Epilamonas, i-Hemophilus influenzae. fluorescens, Candida albicans, Chlamydia pneumoniae, Respiratory Adenovirus, Enterococcus kunye neesampuli zesikhohlela ngaphandle kwethagethi, njl. b)Amandla okuthintela ukuphazamiseka: Khetha i-mucin, i-minocycline, i-gentamicin, i-clindamycin, i-imipenem, i-cefoperazone, i-meropenem, i-ciprofloxacin hydrochloride, i-levofloxacin, i-clavulanic acid, kunye ne-roxithromycin, njl. kuvavanyo lokuphazamiseka, kwaye iziphumo zibonisa ukuba izinto eziphazamisayo zikhankanywe ngasentla. musa ukuphazamisa ukufunyanwa kwe-Klebsiella pneumoniae, i-Acinetobacter baumannii, i-Pseudomonas aeruginosa kunye ne-carbapenem resistance genes KPC, NDM, OXA48 kunye ne-IMP.
Izixhobo Ezisebenzayo	Iinkqubo ze-Biosystems ezisetyenzisiweyo 7500 Iinkqubo zePCR zeXesha langempela Iinkqubo ze-Biosystems eziSetyenzisiweyo 7500 Iinkqubo zePCR ezikhawulezayo QuantStudio^®IiNkqubo ze-PCR ze-5 zeXesha langempela I-LightCycler^®Inkqubo ye-PCR ye-480 yexesha langempela I-LineGene 9600 Plus ye-Real-Time PCR Detection System (FQD-96A, iteknoloji ye-Bioer) I-MA-6000 yeXesha lokwenyani iBhayisekile yoMyinge weThermal (iSuzhou Molarray Co., Ltd.) I-BioRad CFX96 Inkqubo yePCR yexesha lokwenyani BioRad CFX Opus 96 Real-Time PCR System