I-Klebsiella Pneumoniae, i-Acinetobacter Baumannii kunye ne-Pseudomonas Aeruginosa kunye ne-Drug Resistance Genes (KPC, NDM, OXA48 kunye ne-IMP) Multiplex

Inkcazo emfutshane:

Le khithi isetyenziselwa ukufumanisa iKlebsiella pneumoniae (KPN) kwi-in vitro, i-Acinetobacter baumannii (Aba), i-Pseudomonas aeruginosa (PA) kunye ne-carbapenem resistance genes ezine (eziquka i-KPC, i-NDM, i-OXA48 kunye ne-IMP) kwiisampuli ze-sputum zabantu, ukubonelela ngesiseko sesikhokelo sokuxilongwa kweklinikhi, unyango kunye namayeza kwizigulana ezirhanelwa ukuba zisulelekile yintsholongwane.

Thumela i-imeyile kuthi

Iinkcukacha zeMveliso

Iithegi zeMveliso

Igama lemveliso

I-HWTS-RT109 Klebsiella Pneumoniae, i-Acinetobacter Baumannii kunye ne-Pseudomonas Aeruginosa kunye ne-Drug Resistance Genes (i-KPC, i-NDM, i-OXA48 kunye ne-IMP) Multiplex Detection Kit (i-Fluorescence PCR)

Isatifikethi

I-Epidemiology

I-Klebsiella pneumoniae yintsholongwane eqhelekileyo ebangela amathuba kwaye yenye yeentsholongwane ezibalulekileyo ezibangela usulelo lwe-nosocomial. Xa ukumelana komzimba kunciphile, iintsholongwane zingena emiphungeni zisuka kwindlela yokuphefumla, zibangele usulelo kwiindawo ezininzi zomzimba, kwaye ukusetyenziswa kwangoko kwee-antibiotics ngundoqo wokunyanga.^[1].

Eyona ndawo ixhaphakileyo yosulelo lwe-Acinetobacter baumannii yimiphunga, eyintsholongwane ebalulekileyo kwi-Hospital acquired pneumonia (HAP), ingakumbi i-Ventilator associated pneumonia (VAP). Ihlala ihamba nezinye izifo zebhaktiriya kunye nefungus, ezineempawu zesantya esiphezulu sokugula kunye nesantya esiphezulu sokufa.

I-Pseudomonas aeruginosa yeyona bacilli ixhaphakileyo engeyiyo i-gram-negative kwizonyango, kwaye yintsholongwane ebalulekileyo enobungozi kwizifo ezifunyanwa esibhedlele, eneempawu zokulula ukungena ekoloni, ukuhluka okulula kunye nokuxhathisa amayeza amaninzi.

Itshaneli

Igama	Umxube we-PCR 1	Umxube we-PCR 2
Itshaneli yeFAM	Aba	I-IMP
Itshaneli yeVIC/HEX	Ulawulo lwangaphakathi	I-KPC
Itshaneli ye-CY5	PA	I-NDM
Itshaneli yeROX	I-KPN	I-OXA48

Iiparameters zobugcisa

Indawo yokugcina izinto	≤-18℃
Beka ubomi kwishelufa	Iinyanga ezili-12
Uhlobo lweSibonelelo	I-sputum
Ct	≤36
CV	≤10.0%
I-LoD	1000 CFU/mL
Ukuchaneka	a)Uvavanyo lwe-cross-reactivity lubonisa ukuba le khithi ayinayo i-cross reactivity nezinye izifo ezibangela ukuphefumla, ezifana neStreptococcus pneumoniae, Neisseria meningitidis, Staphylococcus aureus, Klebsiella oxytoca, Haemophilus influenzae, Acinetobacter jelly, Acinetobacter hemolytica, Legionella pneumophila, Escherichia coli, Pseudomonas fluorescens, Candida albicans, Chlamydia pneumoniae, Respiratory Adenovirus, Enterococcus kunye neesampuli ze-sputum ezingenazo iithagethi, njl. b) Amandla okulwa nokuphazamiseka: Khetha i-mucin, i-minocycline, i-gentamicin, i-clindamycin, i-imipenem, i-cefoperazone, i-meropenem, i-ciprofloxacin hydrochloride, i-levofloxacin, i-clavulanic acid, kunye ne-roxithromycin, njl. ukuze kuvavanywe ukuphazamiseka, kwaye iziphumo zibonisa ukuba izinto zokuphazamiseka ezikhankanyiweyo apha ngasentla aziphazamisi ukufunyanwa kwe-Klebsiella pneumoniae, i-Acinetobacter baumannii, i-Pseudomonas aeruginosa kunye ne-carbapenem resistance genes KPC, NDM, OXA48 kunye ne-IMP.
Izixhobo Ezisebenzayo	Iinkqubo ze-PCR ze-Applied Biosystems 7500 Real-Time Iinkqubo ze-PCR eziSebenzisiweyo ze-7500 ezikhawulezayo zeXesha langempela I-QuantStudio^®Iinkqubo ezi-5 ze-PCR zeXesha langempela I-LightCycler^®Inkqubo ye-PCR yeXesha langempela ye-480 Inkqubo yokuFumanisa i-LineGene 9600 Plus yeXesha langempela le-PCR (i-FQD-96A, iteknoloji ye-Bioer) I-MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) Inkqubo ye-PCR ye-BioRad CFX96 yeXesha langempela Inkqubo ye-PCR ye-BioRad CFX Opus 96 yeXesha langempela