Isifo sokuphefumla saseMiddle East iCoronavirus Nucleic Acid
Igama lemveliso
I-HWTS-RT031A-I-Middle East Respiratory Syndrome Coronavirus Nucleic Acid Detection Kit (i-Fluorescent PCR)
I-Epidemiology
I-Middle East respiratory syndrome coronavirus (MERS-CoV), i-β-coronavirus ebangelaisifo sokuphefumla ebantwini, saqala ukubonwa kwisigulana esiyindoda esineminyaka engama-60 ubudala saseSaudi Arabia ngomhla wama-24 kuJulayi, ngo-2012. Imbonakalo yeklinikhi yosulelo lwe-MERS-CoV isusela kwimeko engenazimpawu okanye iimpawu zokuphefumla ezibuthathaka ukuya kwisifo sokuphefumla esiqatha kwanokufa.
Itshaneli
| I-FAM | I-RNA yentsholongwane ye-MERS |
| I-VIC(HEX) | Ulawulo lwangaphakathi |
Iiparameters zobugcisa
| Indawo yokugcina izinto | ≤-18℃ Ebumnyameni |
| Beka ubomi kwishelufa | Iinyanga ezi-9 |
| Uhlobo lweSibonelelo | Iiswabhu ze-nasopharyngeal eziqokelelweyo ezisandula ukuqokelelwa |
| CV | ≤5.0% |
| Ct | ≤38 |
| I-LoD | Iikopi ezili-1000/mL |
| Ukuchaneka | Akukho kudibana kwe-coronavirus yabantu i-coronavirus yabantu i-SARSr-CoV kunye nezinye izifo ezixhaphakileyo. |
| Izixhobo Ezisebenzayo: | Iinkqubo ze-PCR ze-Applied Biosystems 7500 Real-Time Iinkqubo ze-PCR eziSebenzisiweyo ze-7500 ezikhawulezayo zeXesha langempela Iinkqubo ze-PCR zeQuantStudio®5 zeXesha langempela Iinkqubo ze-PCR ze-SLAN-96P zeXesha langempela Inkqubo ye-PCR yeLightCycler®480 yeXesha langempela Inkqubo yokuHlola i-PCR yeLineGene 9600 Plus ngexesha langempela I-MA-6000 Real-Time Quantitative Thermal Cycler Inkqubo ye-PCR ye-BioRad CFX96 yeXesha langempela Inkqubo ye-PCR ye-BioRad CFX Opus 96 yeXesha langempela |
Ukuhamba komsebenzi
Ukhetho 1.
I-reagent yokukhupha ecetyiswayo: I-QIAamp Viral RNA Mini Kit (52904), i-Nucleic Acid Extraction okanye i-Purification Reagent (YDP315-R) yiTiangen Biotech (Beijing) Co., Ltd.
Ukhetho 2.
I-reagent yokukhupha ecetyiswayo: I-Macro & Micro-Test General DNA/RNA Kit (HWTS-3017) kunye ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B).
Iindidi zemveliso
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Iintlobo ezili-19 ze-Respiratory Pathogen Nucleic Acid
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Iintlobo ezi-4 zeentsholongwane zokuphefumla i-Nucleic Acid
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I-Antigen yeVirus ehambelanayo yokuphefumla
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Iintlobo ezintandathu zezifo zokuphefumla
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I-AdV Universal kunye ne-Type 41 Nucleic Acid
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I-Influenza A Virus Nucleic Acid
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I-Influenza A/B Antigen
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I-Influenza B Virus Nucleic Acid
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I-Antigen ye-Adenovirus
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I-Mycoplasma Pneumoniae Nucleic Acid