Intsholongwane ye-Monkeypox kunye nokuchwetheza i-nucleid acid

Intsholongwane ye-Monkeypox kunye nokuchwetheza i-nucleic acid ibonisa umfanekiso

Inkcazo emfutshane:

Le khit isetyenziselwa ukubongwa kweVitroypote ye-vitroypox Vide i, i-bide i-II kunye ne-Monkeypox intsholongwane i-acid acid i-acid ye-acid ye-Acid ye-Acid, i-Oropharyngeal Swabs kunye neesampulu ze-seroberyngezwa.

Thumela i-imeyile kuthi

Iinkcukacha zemveliso

Iimpawu zeMveliso

Igama lemveliso

Intsholongwane ye-HWTS-OT202-i-Monkeypox intsholongwane kunye nokuchwetheza i-nucleid i-acid ikhitshi yekhitshi (iFluorescence PCR)

Isatifiketi

Isipikumu

I-Monkeypox (i-MPOX) sisifo esosulelayo se-soonotic soosonotic esibangelwa yintsholongwane ye-Monkeypox (MPXV). I-MPXV ijikelezwe kabini okanye i-oval yemozulu, kwaye yintsholongwane ye-DNA ephindwe kabini enemitha enye malunga ne-197KB^[1]. Esi sifo sidluliselwa zizilwanyana, kwaye abantu banokusulwa zilunywa zizilwanyana ezosulelekileyo okanye ngokudibana ngqo negazi, ulwelo lomzimba kunye nokuvutha kwezilwanyana ezosulelekileyo. Intsholongwane inokudluliselwa phakathi kwabantu, ngokuyintloko ngamathontsi aphezulu ngexesha elide, ukunxibelelana ngqo nobuso okanye ngokudibana ngqo nolwelo kunye nolwelo lomzimba okanye izinto ezingcolisekileyo^[2-3]. Izifundo zibonakalise ukuba i-MPXV inee-clade ezimbini ezahlukileyo: clade i (ngaphambili yaziwa ngokuba yi-African African Clalsi) i-Clade i-II (ngaphambili ibiza i-Clade ye-Afrika yaseNtshona). I-MPOX ye-CANO BOCIN CLADE iboniswe ngokucacileyo ukuba ithe yabethelwa phakathi kwabantu kwaye inokubangela ukuba i-MPOX ye-African African ibangela impawu zomzimba kwaye inesilinganiselo esisezantsi sosulelo lomntu^[4].

Iziphumo zovavanyo zale kit azijongwa njengezona nqaku kukufumana usulelo lwe-MPXV kwizigulana, ezifanele zidityaniswe neempawu zovavanyo zesigulana kunye nezinye iilebhu zokugweba ngokufanelekileyo unyango kunye nokwenza unyango olululo Cwangcisa ukuqinisekisa unyango olukhuselekileyo nolusebenzayo.

Ijelo

Indlala	I-MPXV Clade II
Rox	I-MPXV i-nurt nucleid acid
I-vic / hex	I-MPXV clade i
I-Cy5	Ulawulo lwangaphakathi

Iiparamitha zobugcisa

Ukugcinwa	≤-18 ℃
Beka ubomi kwishelufa	Iinyanga ezili-12
Uhlobo locaphu	I-rash rash fluid, i-oropharyngezwaal swabs kunye ne-serum
Ct	¶338 (IFAM, VIV / VIC / HeX), ROX), ¶35 (IC)
Indawo yokuhlala	Iikopi ezingama-200 / ml
Izixhobo ezifanelekileyo	Chwetheza i-Reagent: Faka isicelo se-biosystemsts 700 ye-PCR ye-PCR ye-PCR Faka isicelo se-biosystemsts ezingama-700 ngokukhawuleza kwe-PCR ye-PCR Ubungakanani^®Iinkqubo ezi-5 zokwenyani ze-PCR I-Slan-96p yangempela ye-PCR ye-PCR (Hongshi yonyango lweTekhnoloji Co., Ltd.) Ukukhanya^®I-480 yeyokwenene ye-PCR I-Linegene 9600 kunye neenkqubo zokufumana i-PRRE-96A-96a, i-Hangzhou Bioudecteech yeBikoer) I-Ma-6000 yexesha lokwenyani le-thermal ye-thermal (Suzhou Molarray Co., Ltd.) I-biorad cfx96 yeyokwenyani ye-PFRR I-biorad cfx opi 96 Inkqubo ye-PFR ye-PCR Uhlobo lokufumana i-II Reagent: Eudmon^TMAIO800 (HWTS-EQ007)