I-Monkeypox Virus kunye nokuChwetheza i-Nucleic Acid

Intsholongwane yeMonkeypox kunye nokuChwetheza iNucleic Acid eFakelweyo Umfanekiso

Inkcazelo emfutshane:

Le kit isetyenziselwa in vitro ubhaqo qualitative of monkeypox virus clade I, clade II kunye monkeypox virus universal nucleic acids kulwelo human rash, oropharyngeal swabs kunye neesampulu serum.

Thumela i-imeyile kuthi

Iinkcukacha zeMveliso

Iithegi zeMveliso

Igama lemveliso

HWTS-OT202-Monkeypox Virus kunye nokuChwetheza iNucleic Acid Detection Kit (Fluorescence PCR)

Isatifikethi

I-Epidemiology

I-Monkeypox (i-Mpox) sisifo esosulelayo se-zoonotic esibangelwa yi-Monkeypox Virus (MPXV). IMPXV inezitena ezingqukuva okanye imile okweqanda, kwaye iyintsholongwane ephindwe kabini yeDNA enobude obungange 197Kb.^[1]. Ubukhulu becala esi sifo sisasazwa zizilwanyana, yaye abantu banokosulelwa ngokulunywa zizilwanyana ezosulelekileyo okanye ngokudibana ngokuthe ngqo negazi, ulwelo lomzimba nerhashalala lezilwanyana ezosulelekileyo. Intsholongwane inokosulela phakathi kwabantu, ikakhulu ngamathontsi okuphefumla ngexesha elide, ngqo ubuso ngobuso okanye ngokunxibelelana ngokuthe ngqo nolwelo lomzimba wesigulana okanye izinto ezingcolisekileyo.^[2-3]. Uphononongo lubonise ukuba iMPXV yenza amagaqa amabini ahlukeneyo: iclade I (eyayisaziwa njengeClade yaseCentral African okanye iCongo Basin clade) kunye neclade II (eyayibizwa ngokuba yiWest African clade). I-mpox ye-Congo Basin clade ibonakaliswe ngokucacileyo ukuba idluliselwe phakathi kwabantu kwaye ingabangela ukufa, ngelixa i-pox ye-West African clade ibangela iimpawu ezinobunzima kwaye inomlinganiselo ophantsi wokudluliselwa komntu kumntu.^[4].

Iziphumo zovavanyo lwale khithi azenzelwanga ukuba zibe ngumqondiso wokuphela kokuxilongwa kwe-MPXV yosulelo kwizigulane, ekufuneka zidityaniswe neempawu zeklinikhi zesigulane kunye nezinye iinkcukacha zovavanyo lwebhubhoratri ukugweba ngokuchanekileyo usulelo lwe-pathogen kunye nokwenza isicwangciso sonyango esifanelekileyo ukuqinisekisa unyango olukhuselekileyo nolusebenzayo.

Umjelo

FAM	MPXV clade II
ROX	MPXV i-nucleic acid jikelele
VIC/HEX	MPXV clade I
CY5	ulawulo lwangaphakathi

Iiparamitha zobuGcisa

Ugcino	≤-18℃
Beka ubomi kwishelufa	Iinyanga ezili-12
Uhlobo loMfanekiso	Ulwelo lwerhashalala lomntu, i-oropharyngeal swabs kunye neserum
Ct	≤38 (FAM, VIC/HEX, ROX), ≤35（IC)
LoD	Iikopi ezingama-200/mL
Izixhobo Ezisebenzayo	Chwetheza I-reagent yokubona: Iinkqubo ze-Biosystems ezisetyenzisiweyo 7500 Iinkqubo zePCR zeXesha langempela Iinkqubo ze-Biosystems eziSetyenzisiweyo 7500 Iinkqubo zePCR ezikhawulezayo QuantStudio^®IiNkqubo ze-PCR ze-5 zeXesha langempela SLAN-96P Iinkqubo zePCR zeXesha lokwenyani (Hongshi Medical Technology Co., Ltd.) I-LightCycler^®Inkqubo ye-PCR ye-480 yexesha langempela I-LineGene 9600 Plus Iinkqubo zokuFumana ze-PCR ngexesha langempela (FQD-96A, iteknoloji ye-Hangzhou Bioer) I-MA-6000 yeXesha lokwenyani iBhayisekile yoMyinge weThermal (iSuzhou Molarray Co., Ltd.) I-BioRad CFX96 Inkqubo yePCR yexesha lokwenyani BioRad CFX Opus 96 Real-Time PCR System Uhlobo lwe-II yokufumanisa i-reagent: Eudemon^TMI-AIO800 (HWTS-EQ007)) yi-Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.