Intsholongwane yeMonkeypox kunye neTyping Nucleic Acid

Umfanekiso obonisiweyo weNyukliya yeMonkeypox kunye neTyping Nucleic Acid

Inkcazo emfutshane:

Le khithi isetyenziselwa ukufumanisa i-in vitro qualitative nucleic acids ze-monkeypox virus clade I, clade II kunye ne-monkeypox virus universal nucleic acids kulwelo lwe-rash lomntu, ii-oropharyngeal swabs kunye neesampuli ze-serum.

Thumela i-imeyile kuthi

Iinkcukacha zeMveliso

Iithegi zeMveliso

Igama lemveliso

I-HWTS-OT202-Monkeypox Virus kunye neTyping Nucleic Acid Detection Kit (i-Fluorescence PCR)

Isatifikethi

I-Epidemiology

I-Monkeypox (i-Mpox) sisifo esosulelayo esibangelwa yi-Monkeypox Virus (MPXV). I-MPXV inezitena ezingqukuva okanye ezimile okweqanda, kwaye yintsholongwane ye-DNA enemicu emibini enobude obumalunga ne-197Kb.^[1]Esi sifo sidluliselwa kakhulu zizilwanyana, kwaye abantu banokusuleleka ngokulunywa zizilwanyana ezinesi sifo okanye ngokudibana ngqo negazi, ulwelo lomzimba kunye nokurhawuzelelwa kwezilwanyana ezinesi sifo. Le ntsholongwane ingadluliselwa phakathi kwabantu, ikakhulu ngamathontsi okuphefumla ngexesha lokudibana ubuso ngobuso ixesha elide okanye ngokudibana ngqo nolwelo lomzimba wesigulana okanye izinto ezinesi sifo.^[2-3]Izifundo zibonise ukuba i-MPXV yakha ii-clade ezimbini ezahlukeneyo: i-clade I (eyayisaziwa ngokuba yi-Central African clade okanye i-Congo Basin clade) kunye ne-clade II (eyayisaziwa ngokuba yi-West African clade). I-mpox ye-Congo Basin clade ibonakalisiwe ngokucacileyo ukuba inokudluliselwa phakathi kwabantu kwaye inokubangela ukufa, ngelixa i-mpox ye-West African clade ibangela iimpawu ezibuthathaka kwaye inezinga eliphantsi lokudluliselwa kwabantu ukusuka ebantwini ukuya ebantwini.^[4].

Iziphumo zovavanyo zale khithi azijoliswanga ekubeni zizibonakaliso zodwa zokuchongwa kosulelo lwe-MPXV kwizigulana, ekufuneka zidityaniswe neempawu zeklinikhi zesigulana kunye nolunye ulwazi lovavanyo lwelabhoratri ukuze kugwetywe ngokuchanekileyo usulelo lwe-pathogen kwaye kwenziwe isicwangciso sonyango esifanelekileyo ukuqinisekisa unyango olukhuselekileyo nolusebenzayo.

Itshaneli

I-FAM	I-MPXV clade II
I-ROX	I-MPXV i-nucleic acid yendalo yonke
I-VIC/HEX	I-MPXV clade I
CY5	ulawulo lwangaphakathi

Iiparameters zobugcisa

Indawo yokugcina izinto	≤-18℃
Beka ubomi kwishelufa	Iinyanga ezili-12
Uhlobo lweSibonelelo	ulwelo lwerhashalala ebantwini, ii-oropharyngeal swabs kunye ne-serum
Ct	≤38 (FAM, VIC/HEX, ROX), ≤35 (IC)
I-LoD	Iikopi ezingama-200/mL
Izixhobo Ezisebenzayo	Isixhobo sokuchonga uhlobo lokuqala: Iinkqubo ze-PCR ze-Applied Biosystems 7500 Real-Time Iinkqubo ze-PCR eziSebenzisiweyo ze-7500 ezikhawulezayo zeXesha langempela I-QuantStudio^®Iinkqubo ezi-5 ze-PCR zeXesha langempela Iinkqubo ze-PCR ze-SLAN-96P zeXesha langempela (iHongshi Medical Technology Co., Ltd.) I-LightCycler^®Inkqubo ye-PCR yeXesha langempela ye-480 Iinkqubo zokuFumanisa i-LineGene 9600 Plus Real-Time PCR (FQD-96A, iteknoloji ye-Hangzhou Bioer) I-MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) Inkqubo ye-PCR ye-BioRad CFX96 yeXesha langempela Inkqubo ye-PCR ye-BioRad CFX Opus 96 yeXesha langempela I-reagent yokuchonga yohlobo lwesibini: U-Eudemon^TMI-AIO800 (HWTS-EQ007)) yiJiangsu Macro kunye neMicro-Test Med-Tech Co., Ltd.