I-Nucleic Acid yeNtsholongwane yeMonkeypox Virus
Igama lemveliso
Ikhithi yokuFumanisa iNucleic Acid yeNtsholongwane yeHWTS-OT071-Monkeypox Virus (iFluorescence PCR)
I-HWTS-OT078-Isixhobo sokuFumanisa i-Nucleic Acid yeMonkeypox eyomileyo ngokuQhotsa (i-Fluorescence PCR)
Isatifikethi
CE
I-Epidemiology
I-Monkeypox (MP) sisifo esosulelayo esibangelwa yiMonkeypox Virus (MPV). Esi sifo sidluliselwa kakhulu zizilwanyana, kwaye abantu banokusuleleka ngokulunywa zizilwanyana ezineentsholongwane okanye ngokudibana ngqo negazi, ulwelo lomzimba kunye nokurhawuzelelwa kwezilwanyana ezineentsholongwane. Le ntsholongwane ingadluliselwa phakathi kwabantu, ngokuyintloko ngamathontsi okuphefumla ngexesha lokudibana ubuso ngobuso ixesha elide okanye ngokudibana ngqo nolwelo lomzimba wesigulana okanye izinto ezineentsholongwane.
Iimpawu zeklinikhi zosulelo lwe-monkeypox ebantwini ziyafana nezo ze-smallpox, ngokubanzi emva kwexesha lokufunxa iintsuku ezili-12, kuvela umkhuhlane, intloko ebuhlungu, iintlungu zemisipha nomqolo, ukwanda kwama-lymph nodes, ukudinwa kunye nokungonwabi. Ukuqhambuka kubonakala emva kweentsuku ezi-1-3 zomkhuhlane, ngokuqhelekileyo kuqala ebusweni, kodwa nakwezinye iindawo. Ikhosi yesifo ngokubanzi ihlala iiveki ezi-2-4, kwaye izinga lokufa yi-1%-10%. I-Lymphadenopathy yenye yezona zinto ziphambili umahluko phakathi kwesi sifo kunye ne-smallpox.
Itshaneli
| Itshaneli | I-Monkeypox |
| I-FAM | I-gene ye-MPV-1 yentsholongwane yeMonkeypox |
| I-VIC/HEX | I-gene ye-MPV-2 yentsholongwane yeMonkeypox |
| I-ROX | / |
| CY5 | Ulawulo lwangaphakathi |
Iiparameters zobugcisa
| Indawo yokugcina izinto | Ulwelo: ≤-18℃ Ebumnyameni; I-Lyophilized: ≤30℃ Ebumnyameni |
| Beka ubomi kwishelufa | Iinyanga ezili-12 |
| Uhlobo lweSibonelelo | Ulwelo lwerhashalala, I-Nasopharyngeal Swab, I-throat Swab, I-Serum |
| Ct | ≤38 |
| CV | ≤5.0％ |
| I-LoD | Iikopi ezingama-200/mL |
| Ukuchaneka | Akukho kudibana kwe-Smallpox virus, iCowpox virus, iVaccinia virus, iHerpes simplex virus, njl. Akukho kudibana kwe-trans-reactivity nezinye iintsholongwane ezibangela isifo sokurhawuzelela. Akukho kudibana kwe-DNA ye-genomic yomntu. |
| Izixhobo Ezisebenzayo | Ingalingana nezixhobo zePCR ezikhanyayo ezithengiswayo. Iinkqubo ze-ABI 7500 ze-Real-Time PCR Iinkqubo ze-PCR zeQuantStudio® 5 zeXesha langempela Iinkqubo ze-PCR ze-SLAN-96P zeXesha langempela Inkqubo ye-PCR yeLightCycler®480 yeXesha langempela Inkqubo yokuHlola i-PCR yeLineGene 9600 Plus ngexesha langempela I-MA-6000 Real-Time Quantitative Thermal Cycler |
Isisombululo se-PCR iyonke
