I-Nucleic Acid yeNtsholongwane yeMonkeypox Virus

Umfanekiso obonisiweyo weNyukleyi yeVirus yeMonkeypox

Inkcazo emfutshane:

Le khithi isetyenziselwa ukufumanisa i-nucleic acid ye-monkeypox virus kwisampuli yolwelo lwerhashalala lomntu kunye ne-oropharyngeal swab.

Thumela i-imeyile kuthi

Iinkcukacha zeMveliso

Iithegi zeMveliso

Igama lemveliso

Ikhithi yokuFumana i-HWTS-OT200 Monkeypox Virus Nucleic Acid (i-Enzymatic Probe Isothermal Amplification)

Isatifikethi

I-Epidemiology

I-Monkeypox (MPX) sisifo esosulelayo esibangelwa yiMonkeypox Virus (MPXV). I-MPXV inemilo engqukuva okanye imile okweqanda, kwaye yintsholongwane ye-DNA enemicu emibini enobude obuyi-197Kb. Esi sifo sidluliselwa ikakhulu zizilwanyana, kwaye abantu banokusuleleka ngokulunywa zizilwanyana ezineentsholongwane okanye ngokudibana ngqo negazi, ulwelo lomzimba kunye nokurhawuzelelwa kwezilwanyana ezineentsholongwane. Intsholongwane ingadluliselwa phakathi kwabantu, ikakhulu ngamathontsi okuphefumla ngexesha lokudibana ubuso nobuso ixesha elide okanye ngokudibana ngqo nolwelo lomzimba wesigulana okanye izinto ezineentsholongwane. Iimpawu zeklinikhi zosulelo lwe-monkeypox ebantwini ziyafana nezo ze-smallpox, ngokubanzi emva kwexesha lokufunxa iintsuku ezili-12, ukubonakala komkhuhlane, intloko ebuhlungu, iintlungu zemisipha kunye nomqolo, ukwanda kwee-lymph nodes, ukudinwa kunye nokungonwabi. Ukurhawuzelelwa kubonakala emva kweentsuku ezi-1-3 zomkhuhlane, ngokuqhelekileyo kuqala ebusweni, kodwa nakwezinye iindawo. Ikhosi yesifo ihlala iiveki ezi-2-4, kwaye izinga lokufa liyi-1%-10%. I-Lymphadenopathy yenye yezona zinto ziphambili ezahlukileyo kwesi sifo kunye ne-smallpox.

Iziphumo zovavanyo zale khithi akufuneki zisetyenziswe njengesalathisi sodwa sokuchongwa kosulelo lwentsholongwane ye-monkeypox kwizigulana, ekufuneka zidityaniswe neempawu zeklinikhi zesigulana kunye nolunye ulwazi lovavanyo lwelabhoratri ukuze kuchongwe ngokuchanekileyo usulelo lwentsholongwane, kwaye kwenziwe isicwangciso sonyango esifanelekileyo sokwenza unyango lukhuseleke kwaye lusebenze.

Iiparameters zobugcisa

Uhlobo lweSibonelelo	ulwelo lwerhashalala lomntu, i-oropharyngeal swab
Itshaneli	I-FAM
Tt	28
CV	≤5.0%
I-LoD	Iikopi ezingama-200/μL
Ukuchaneka	Sebenzisa ikhithi ukubona ezinye iintsholongwane, ezifana ne-Smallpox virus, i-Cowpox virus, i-Vaccinia virus,Intsholongwane yeHerpes simplex, njl.njl., kwaye akukho mpendulo idibeneyo.
Izixhobo Ezisebenzayo	Inkqubo yokuFumanisa i-Easy Amp Real-time Fluorescence Isothermal (HWTS 1600) Iinkqubo ze-PCR ze-Applied Biosystems 7500 Real-Time, I-QuantStudio^®Iinkqubo ezi-5 ze-PCR zeXesha langempela Iinkqubo ze-PCR ze-SLAN-96P zeXesha langempela Iinkqubo zokuFumanisa i-LineGene 9600 Plus zeXesha langempela ze-PCR I-MA-6000 Real-Time Quantitative Thermal Cycler Iinkqubo ze-PCR zeBioRad CFX96 zeXesha langempela, Iinkqubo ze-PCR zeBioRad CFX Opus 96 zeXesha langempela.