I-Monkeypox Virus Nucleic Acid

Monkeypox Virus Nucleic Acid Umfanekiso obonakalayo

Inkcazelo emfutshane:

Le khithi isetyenziselwa ukufumanisa umgangatho we-in vitro we-monkeypox virus nucleic acid kwi-rash fluid kunye neesampuli ze-oropharyngeal swab.

Thumela i-imeyile kuthi

Iinkcukacha zeMveliso

Iithegi zeMveliso

Igama lemveliso

I-HWTS-OT200 Monkeypox Virus Nucleic Acid Detection Kit(Enzymatic Probe Isothermal Amplification)

Isatifikethi

I-Epidemiology

I-Monkeypox (MPX) sisifo esosulelayo se-zoonotic esibangelwa yi-Monkeypox Virus (MPXV). I-MPXV inezitena ezingqukuva okanye i-oval imile, kwaye iyintsholongwane ye-DNA enemisonto ephindwe kabini enobude obumalunga ne-197Kb. Ubukhulu becala esi sifo sisasazwa zizilwanyana, yaye abantu banokosulelwa ngokulunywa zizilwanyana ezosulelekileyo okanye ngokudibana ngokuthe ngqo negazi, ulwelo lomzimba nerhashalala lezilwanyana ezosulelekileyo. Intsholongwane inokosulela phakathi kwabantu, ikakhulu ngamathontsi okuphefumla ngexesha elide, ngqo ubuso ngobuso okanye ngokudibana ngokuthe ngqo nolwelo lomzimba wesigulana okanye izinto ezisulelekileyo. Iimpawu zeklinikhi zosulelo lwemonkeypox ebantwini zifana nezo ze-smallpox, ngokubanzi emva kwexesha le-12-day incubation, ukubonakala komkhuhlane, intloko ebuhlungu, iintlungu zemisipha kunye nomqolo, i-lymph nodes eyandisiweyo, ukukhathala kunye nokungahambi kakuhle. I-rash ibonakala emva kweentsuku ezi-1-3 zomkhuhlane, ngokuqhelekileyo kuqala ebusweni, kodwa nakwezinye iindawo. Ikhosi yesifo ngokubanzi ithatha iiveki ezi-2-4, kwaye izinga lokufa li-1% -10%. I-Lymphadenopathy yenye yeeyantlukwano eziphambili phakathi kwesi sifo kunye ne-smallpox.

Iziphumo zovavanyo lwale khithi akufuneki zisetyenziswe njengesalathiso kuphela sokuxilongwa kosulelo lwentsholongwane yemonkeypox kwizigulana, ekufuneka zidityaniswe neempawu zeklinikhi zesigulane kunye nezinye iidatha zovavanyo lwebhubhoratri ukufumanisa ngokuchanekileyo usulelo lwe-pathogen, kwaye yenza isicwangciso sonyango esifanelekileyo ukwenza unyango lukhuseleke kwaye lusebenze.

IiParameters zobuGcisa

Uhlobo loMfanekiso	Ulwelo lwerhashalala lomntu, i-oropharyngeal swab
Umjelo	FAM
Tt	28
CV	≤5.0%
LoD	Iikopi ezingama-200/μL
Ukuchaza ngokuthe ngqo	Sebenzisa ikhithi ukubona ezinye iintsholongwane, ezifana nentsholongwane yengqakaqha, intsholongwane yeCowpox, iVaccinia virus,Intsholongwane ye-Herpes simplex, njl., kwaye akukho mpendulo yomnqamlezo.
Izixhobo Ezisebenzayo	I-Easy Amp Real-time Fluorescence Isothermal Detection System (HWTS 1600) Iinkqubo ze-Biosystems ezisetyenzisiweyo 7500 Iinkqubo zePCR zeXesha langempela, QuantStudio^®IiNkqubo ze-PCR ze-5 zeXesha langempela SLAN-96P Iinkqubo zePCR zeXesha langempela LineGene 9600 Plus Real-Time PCR Ukufunyanwa Systems MA-6000 Real-Time Quantitative Thermal Cycler IiNkqubo zePCR zeBioRad CFX96 zeXesha lokwenyani, BioRad CFX Opus 96 Real-Time PCR Systems.