Intsholongwane ye-Influenza A/ Intsholongwane ye-Influenza B
Igama lemveliso
I-HWTS-RT176-I-Influenza A Virus/Influenza B Virus eyomileyo eqandisiweyo i-Nucleic Acid DetectionKit (i-Fluorescence PCR)
Ukuvunywa kweMveliso
IVDR
I-Epidemiology
Ngokwee-antigenic differences phakathi kwe-NP kunye ne-M genes, ii-influenza virusesinokwahlulwa ibe ziintlobo ezine: intsholongwane ye-influenza A (IFV A), intsholongwane ye-influenza B (IFV B),intsholongwane yomkhuhlane C (IFV C) kunye nentsholongwane yomkhuhlane D (IFV D) [1]. Kwintsholongwane yomkhuhlane A,Inee-host ezininzi kunye nee-serotypes ezintsonkothileyo kwaye inokusasazeka kuzo zonke ii-hosts ngokusebenzisaukuhlanganiswa kwezakhi zofuzo kunye nokuguqulwa kwezakhi zofuzo okuguquguqukayo. Abantu abanakho amandla okuzikhusela ahlala ixesha elideintsholongwane ye-influenza A, ngoko ke abantu bayo yonke iminyaka ngokubanzi basengozini.zezona zifo ziphambili ezibangela ubhubhane womkhuhlane [2]. Kwintsholongwane yomkhuhlane B,Isasazeka kakhulu kwiindawo ezincinci kwaye okwangoku ayinazo iintlobo ezahlukeneyo.Usulelo lubangelwa kakhulu ziintsholongwane zomkhuhlane ze-B/Yamagata okanye i-B/VictoriaPhakathi kweziganeko zomkhuhlane eziqinisekisiweyo ngenyanga kumazwe ali-15 e-Ummandla waseAsia-Pacific, izinga lokufunyaniswa kwentsholongwane ye-influenza B liqala kwi-0 ukuya kwi-92%[3]. Ngokungafaniyo nentsholongwane ye-influenza A, amaqela athile abantu, anjengabantwana kunyeabantu abadala, basengozini yokuhlaselwa yintsholongwane ye-influenza B, nto leyo enokubangela iingxaki ngokulula,ezibangela umthwalo ongaphezulu kuluntu kunentsholongwane ye-influenza A[4].Iziphumo ezingezizo ezibonisa ukuba i-IFV A/B ayinalo usulelo kwaye akufuneki zisetyenziswe njenge-isiseko sodwa sokuxilongwa, unyango, okanye ezinye izigqibo zolawulo.Iziphumo azithinteli usulelo lwebhaktiriya okanye olunye usulelo lwentsholongwane oluhambisanayo ngaphandle kwe-IFVIntsholongwane ye-A/B.
Iiparameters zobugcisa
| Indawo yokugcina izinto | 2-28℃ |
| Beka ubomi kwishelufa | Iinyanga ezili-12 |
| Uhlobo lweSibonelelo | iswabhu yomlomo womntu, iswabhu yasempumlweni |
| Ct | ≤38 |
| CV | <5.0% |
| I-LoD | Iikopi ezingama-200/mL okanye i-0.05TCID50/mL |
| Ukuchaneka | Ukudibana kwe-Cross-reactivity: Akukho kudibana kwe-kit kunye ne-Bocavirus, i-Respiratory syncytial virus type B, i-Herpes simplex virus type 1, i-Enterovirus, i-Adenovirus (Uhlobo 1), i-Adenovirus (Uhlobo 3), i-Adenovirus (Uhlobo 7), i-SARS-CoV, i-Adenovirus (Uhlobo 1), i-Adenovirus (Uhlobo 3), i-Adenovirus (Uhlobo 7), i-SARS-CoV, i-Norovirus, i-Streptococcus pneumoniae, i-Legionella, i-Bordetella pertussis, i-Neisseria gonorrhoeae, i-Aspergillus fumigatus, i-Moraxella catarrhalis, i-Rhinovirus, i-Parainfluenza virus (Uhlobo 1), i-Parainfluenza virus (Uhlobo 3), i-Parainfluenza virus (Uhlobo 2), i-Varicella-zoster virus, i-Masles virus, i-Human coronaviruses, i-MERS-CoV, i-Chlamydia pneumoniae, i-Klebsiella pneumoniae, i-Pneumocystis jirovecii, i-Staphylococcus aureus, i-Candida ii-albicans, ii-Cryptococcus neoformans, i-Lactobacillus, i-Cytomegalovirus, i-Epstein-Barr virus, i-Mumps virus, i-Human metapneumovirus (A1), i-Human metapneumovirus (B2), i-coronavirus entsha ka-2019-nCoV, i-Rotavirus, i-Mycoplasma pneumoniae, i-Streptococcus pyogenes, i-Haemophilus influenzae, i-Mycobacterium tuberculosis, i-Candida glabrata, i-Streptococcus salivarius, i-Corynebacterium, i-Influenza A virus, i-Influenza B virus, i-Human genomic DNA |
| Izixhobo Ezisebenzayo | Uhlobo I: Iinkqubo ze-PCR ze-Applied Biosystems 7500 Real-Time, SLAN-96PIinkqubo zePCR zeXesha langempela (iHongshi Medical Technology Co., Ltd.). Uvavanyo lohlobo lwesibini: EudemonTM AIO800 (HWTS-EQ007) nguJiangsu Macro kunye Uvavanyo oluNcinci lweMed-Tech Co., Ltd. |
Ukuhamba komsebenzi
Uhlobo I: Kwinkqubo ye-PCR eqhelekileyo
Uhlobo II: Kwi-EudemonTMI-AIO800
IiReagents/Izixhobo ziyafuneka kodwa azibonelelwanga
1) Isixhobo sokuKhupha i-RNA: Isixhobo se-DNA/RNA se-Macro kunye ne-Macro kunye ne-Micro-Test Viral(HWTS-3017)(engasetyenziswa ngeMacro kunye neMicro-Test Automatic NucleicI-Acid Extractor (HWTS-EQ011)) yiJiangsu Macro kunye neMicro-Test Med-Tech Co.,Ltd., iMacro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-8) (isetyenziswa kunyeI-EudemonTM AIO800 (HWTS-EQ007) yiJiangsu Macro kunye neMicro-Test Med-TechI-Co., Ltd.
2) Isisombululo Sokulondolozwa Kwesampulu: Isixhobo Sokuthatha Iisampuli Zeentsholongwane (JXZZ20182400236) nguI-Yocon Biology (Beijing) Co., Ltd., UTM® Universal Transport Medium™(GXB20191277) yiCopan Italia SpA, iMacro kunye neMicro-Test Sample Collection,Ikhithi yokuthumela iposi kunye nokuthumela (HWTS-3002) yiJiangsu Macro kunye neMicro-Test Med-TechI-Co., Ltd.
Izinto ezisetyenziswayo kufuneka kodwa azibonelelwanga:Iingcebiso ezingenayo i-DNase/RNase, iiglavu ezilahlwayo,Iityhubhu ze-EP ezingenayo i-DNase/RNase.
