I-Mumps Virus Nucleic Acid
Igama lemveliso
I-HWTS-RT029-Mumps Virus Nucleic Acid Detection Kit(Fluorescence PCR)
I-Epidemiology
Intsholongwane kaqwilikana yintsholongwane enye ye-serotype, kodwa i-SH protein gene iguquguquka kakhulu kwiintsholongwane zoqwilikana ezahlukeneyo. Intsholongwane ye-Mumps yahlulahlulwe kwii-genotypes ezili-12 ngokusekelwe kwiiyantlukwano ze-SH protein genes, ezizezi iindidi A, B, C, D, F, G, H, I, J, K, L, kunye ne-N. Ukusasazwa kwe-mumps virus genotypes kuneempawu ezicacileyo zengingqi. Iintlobo ezixhaphakileyo eYurophu ikakhulu zii-genotypes A, C, D, G, kunye no-H; ezona ntlobo zixhaphakileyo eMelika zii-genotypes C, D, G, H, J, and K; ezona ntlobo zixhaphakileyo e-Asiya zii-genotypes B, F, I, kunye no-L; olona hlobo luxhaphakileyo eTshayina yi-genotype F; iintlobo ezixhaphakileyo eJapan naseSouth Korea ziigenotypes B kunye nam ngokulandelelanayo. Akukacaci ukuba olu hlobo lokuchwetheza lwentsholongwane lusekwe kwijini ye-SH lunentsingiselo na kuphando lwesitofu. Okwangoku, Iintlobo zesitofu sokugonya esinciphileyo esisetyenziswa kwihlabathi jikelele ikakhulu yi-genotype A, kwaye izilwa-buhlungu eziveliswa zii-antigens zentsholongwane ezahlukeneyo ze-genotypes ziyakhusela.
Iiparamitha zobuGcisa
| Ugcino | -18℃ |
| Beka ubomi kwishelufa | Iinyanga ezili-9 |
| Uhlobo loMfanekiso | Ukuswabha komqala |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | 1000 Iikopi/mL |
| Izixhobo Ezisebenzayo | Isebenza ukuchwetheza i-reagent yokubona: Iinkqubo ze-Biosystems ezisetyenzisiweyo 7500 Iinkqubo zePCR zeXesha langempela, QuantStudio®Iinkqubo zePCR ezi-5 zeXesha langempela, SLAN-96P Iinkqubo zePCR zeXesha lokwenyani (Hongshi Medical Technology Co., Ltd.), I-LineGene 9600 Plus Iinkqubo zokuFumana ze-PCR ngexesha langempela (i-FQD-96A, iteknoloji ye-Hangzhou Bioer), I-MA-6000 yeXesha lokwenyani iBhayisekile yoMyinge weThermal (iSuzhou Molarray Co., Ltd.), Inkqubo yePCR yeBioRad CFX96 yeXesha lokwenyani, BioRad CFX Opus 96 Real-Time PCR System. Isebenza kuhlobo lwe-II yokufumanisa i-reagent: EudemonTMI-AIO800 (HWTS-EQ007) nguJiangsu Macro kunye neMicro-Test Med-Tech Co., Ltd. |
Ukuhamba komsebenzi
IMacro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (enokuthi isetyenziswe kunye neMacro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)), kunye neMacro & Micro-Test Viral DNA/RNA Kit (HWTS-17u-30)TM I-AIO800 (HWTS-EQ007)) yi-Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.
Umthamo wesampulu ekhutshiweyo yi-200μL kwaye umthamo we-elution ocetyiswayo yi-150μL.
