I-Mumps Virus Nucleic Acid
Igama lemveliso
Ikhithi yokuFumanisa i-HWTS-RT029-Mumps Virus Nucleic Acid (i-Fluorescence PCR)
I-Epidemiology
Intsholongwane ye-mumps yintsholongwane enye ye-serotype, kodwa i-SH protein gene iyahluka kakhulu kwiintsholongwane ezahlukeneyo ze-mumps. I-mumps virus yahlulwe yaba zii-genotypes ezili-12 ngokusekelwe kumahluko kwiijini ze-SH protein, ezizezi: iintlobo A, B, C, D, F, G, H, I, J, K, L, kunye no-N. Ukusasazwa kwee-genotypes ze-mumps virus kuneempawu ezicacileyo zengingqi. Iintlobo ezixhaphakileyo eYurophu ikakhulu zii-genotypes A, C, D, G, kunye no-H; iintlobo eziphambili ezixhaphakileyo eMelika zii-genotypes C, D, G, H, J, kunye no-K; iintlobo eziphambili ezixhaphakileyo e-Asia zii-genotypes B, F, I, kunye no-L; uhlobo oluxhaphakileyo e-China yi-genotype F; iintlobo ezixhaphakileyo e-Japan naseMzantsi Korea zii-genotypes B kunye no-I ngokwahlukeneyo. Akucaci ukuba olu hlobo lwe-SH gene-based virus lunentsingiselo na kuphando lokugonya. Okwangoku, iintlobo ze-live attenuated vaccine ezisetyenziswa kwihlabathi liphela ikakhulu zii-genotype A, kwaye ii-antibodies eziveliswa zii-antigens ze-virus ze-genotypes ezahlukeneyo zikhusela ngokunqamlezayo.
Iiparameters zobugcisa
| Indawo yokugcina izinto | -18℃ |
| Beka ubomi kwishelufa | Iinyanga ezi-9 |
| Uhlobo lweSibonelelo | Iswabhu yomphimbo |
| Ct | ≤38 |
| CV | ≤5.0% |
| I-LoD | Iikopi ezili-1000/mL |
| Izixhobo Ezisebenzayo | Isebenza kwi-type I detection reagent: Iinkqubo ze-PCR ze-Applied Biosystems 7500 Real-Time, I-QuantStudio®Iinkqubo ze-PCR ezi-5 zeXesha langempela, Iinkqubo ze-PCR ze-SLAN-96P zeXesha langempela (iHongshi Medical Technology Co., Ltd.), Iinkqubo zokuFumanisa i-LineGene 9600 Plus Real-Time PCR (FQD-96A, iteknoloji ye-Hangzhou Bioer), I-MA-6000 Real-Time Quantitative Thermal Cycler (iSuzhou Molarray Co., Ltd.), Inkqubo ye-PCR ye-BioRad CFX96 yeXesha langempela, Inkqubo ye-PCR ye-BioRad CFX Opus 96 yeXesha langempela. Isebenza kwi-reagent yokuchonga uhlobo lwesibini: U-EudemonTMI-AIO800 (HWTS-EQ007) yiJiangsu Macro kunye neMicro-Test Med-Tech Co., Ltd. |
Ukuhamba komsebenzi
I-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (engasetyenziswa kunye ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)), kunye ne-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-8) (engasetyenziswa kunye ne-EudemonTM I-AIO800 (HWTS-EQ007)) yiJiangsu Macro kunye neMicro-Test Med-Tech Co., Ltd.
Umthamo wesampulu ekhutshiweyo yi-200μL kwaye umthamo we-elution ocetyiswayo yi-150μL.
