I-CYP2C9 yabantu kunye ne-VKORC1 Gene Polymorphism

Inkcazelo emfutshane:

Le kit iyasebenza kwi-vitro qualitative discovery of polymorphism of CYP2C9*3 (rs1057910, 1075A>C) kunye ne-VKORC1 (rs9923231, -1639G>A) kwi-DNA ye-genomic yeesampuli zegazi lomntu.


Iinkcukacha zeMveliso

Iithegi zeMveliso

Igama lemveliso

I-HWTS-GE014A-Human CYP2C9 kunye ne-VKORC1 Gene Polymorphism Detection Kit (i-Fluorescence PCR)

Isatifikethi

CE/TFDA

I-Epidemiology

I-Warfarin yi-anticoagulant yomlomo esetyenziswa rhoqo kwiiklinikhi ngoku, ejoliswe ikakhulu kuthintelo kunye nonyango lwezifo ze-thromboembolic.Nangona kunjalo, i-warfarin inefestile yonyango elinganiselwe kwaye iyahluka kakhulu phakathi kweentlanga ezahlukeneyo kunye nabantu ngabanye.Izibalo zibonise ukuba umahluko wedosi ezinzileyo kubantu abahlukeneyo unokuba ngaphezu kwamaxesha angama-20.Ukopha okungalunganga kwenzeka kwi-15.2% yezigulane ezithatha i-warfarin ngonyaka, apho i-3.5% iphuhlisa ukopha okubulalayo.Uphononongo lwe-Pharmacogenomic lubonise ukuba i-polymorphism yofuzo ye-enzyme ekujoliswe kuyo i-VKORC1 kunye ne-enzyme ye-metabolic CYP2C9 ye-warfarin yinto ebalulekileyo echaphazela umahluko kwithamo le-warfarin.I-Warfarin yi-inhibitor ethile ye-vitamin K epoxide reductase (VKORC1), kwaye ngaloo ndlela inqanda i-clotting factor synthesis ebandakanya i-vitamin K kwaye ibonelela nge-anticoagulation.Inani elikhulu lezifundo libonise ukuba i-polymorphism yemfuza ye-VKORC1 umgqugquzeli yeyona nto ibalulekileyo echaphazela ugqatso kunye nokwahlukana komntu ngamnye kwidosi efunekayo ye-warfarin.I-Warfarin i-metabolized yi-CYP2C9, kwaye iinguqu zayo zinciphisa kakhulu imetabolism ye-warfarin.Abantu abasebenzisa iwarfarin banomngcipheko ophezulu (uphindwe kabini ukuya kathathu ngaphezulu) lokopha kwibakala lokuqala lokusetyenziswa.

Umjelo

FAM I-VKORC1 (-1639G>A)
CY5 CYP2C9*3
VIC/HEX IC

IiParameters zobuGcisa

Ugcino Ulwelo: ≤-18℃
Beka ubomi kwishelufa Iinyanga ezili-12
Uhlobo loMfanekiso Igazi elitsha le-EDTA le-anticoagulated
CV ≤5.0%
LoD 1.0ng/μL
Ukuchaza ngokuthe ngqo Akukho-cross-reactivity kunye nolunye ulandelelwano oluhambelana kakhulu lwe-genome yomntu (i-CYP2C19 gene yomntu, i-RPN2 gene);ukuguqulwa kwe-CYP2C9*13 kunye ne-VKORC1 (3730G>A) ngaphandle koluhlu lokubona le khithi
Izixhobo Ezisebenzayo Iinkqubo ze-Biosystems ezisetyenzisiweyo 7500 Iinkqubo zePCR zeXesha langempela

Iinkqubo ze-Biosystems eziSetyenzisiweyo 7500 Iinkqubo zePCR ezikhawulezayo

I-QuantStudio®5 Iinkqubo zePCR zeXesha langempela

SLAN-96P Iinkqubo zePCR zeXesha langempela

Inkqubo yePCR yeLightCycler®480 yeXesha langempela

I-LineGene 9600 Plus Real-Time PCR Detection System

MA-6000 Real-Time Quantitative Thermal Cycler

I-BioRad CFX96 Inkqubo yePCR yexesha lokwenyani

BioRad CFX Opus 96 Real-Time PCR System

Ukuhamba komsebenzi

Ukukhutshwa kwe-reagent ecetyiswayo: Macro & Micro-Test Viral DNA/RNA Kit(HWTS-3001, HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) kunye neMacro & Micro-Test Automatic Nucleic Acid Extractor(HWTS- 3006).


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