Mycobacterium Tuberculosis INH Ukuguqulwa
Igama lemveliso
I-HWTS-RT137 Mycobacterium Tuberculosis INH iKit yokuFundisa iNguqu (iCurve eNyibilikayo)
I-Epidemiology
I-Mycobacterium tuberculosis, kungekudala njenge-Tubercle bacillus (TB), yibhaktiriya ebangela isifo sephepha. Okwangoku, amayeza asetyenziswa ngokuqhelekileyo kumgca wokuqala wokulwa nesifo sephepha ziquka i-INH, i-rifampicin kunye ne-hexambutol, njl. Amachiza okulwa nesifo sephepha kumgca wesibini aquka i-fluoroquinolones, i-amikacin kunye ne-kanamycin, njl. Isifo sephepha i-mycobacterium ivelisa ukuxhathisa ngamachiza kumachiza achasene nesifo sephepha, nto leyo izisa imingeni enzima kuthintelo nakunyango lwesifo sephepha.
Umjelo
| FAM | MP nucleic acid |
| ROX | Ulawulo lwangaphakathi |
Iiparamitha zobuGcisa
| Ugcino | ≤-18℃ |
| Beka ubomi kwishelufa | Iinyanga ezili-12 |
| Uhlobo loMfanekiso | isikhohlela |
| CV | ≤5% |
| LoD | Umda wokufumanisa uhlobo lwasendle lwe-INH ibhaktheriya yi-2x103 ibhaktheriya / mL, kunye nomda wokufumanisa iibhaktheriya eziguqukayo yi-2x103 ibhaktheriya / mL. |
| Ukuchaza ngokuthe ngqo | a. Akukho kusabela okunqamlezileyo phakathi kwejenome yomntu, ezinye iintsholongwane ze-mycobacteria ezingekho ntuberculous kunye nenyumoniya ezifunyenwe yile khithi.b. Iziza zotshintsho zezinye iijini ezixhathisa ngamachiza kuhlobo lwasendle lwe-Mycobacterium tuberculosis, ezifana nommandla omisela uxhathiso lwejini ye-rifampicin rpoB, zichongiwe, kwaye iziphumo zovavanyo zabonisa ukungaxhathisi kwi-INH, ebonisa ukuba akukho kuphinda kusebenze kwakhona. |
| Izixhobo Ezisebenzayo | SLAN-96P Iinkqubo zePCR zeXesha langempelaIiNkqubo zePCR zeBioRad CFX96 zeXesha lokwenyaniI-LightCycler480®Inkqubo yePCR yexesha lokwenyani |
Ukuhamba komsebenzi
Ukuba usebenzisa i-Macro & Micro-Test General DNA/RNA Kit (HWTS-3019) (enokuthi isetyenziswe kunye ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) yi-Jiangsu Macro & Micro-Test Med-Tech Extraμ, yongeza inkqubo ye-negative Med-Tech Co., Ltd. Isampulu yesikhohlela kufuneka ivavanywe ngokulandelelana, kwaye yongeze i-10μL yolawulo lwangaphakathi ngokwahlukileyo kulawulo olubi, isampuli yesikhohlela esenziwe ukuba ivavanywe, kwaye amanyathelo alandelayo kufuneka enziwe ngokungqongqo ngokwemiyalelo yokutsalwa. Umthamo wesampulu ekhutshiweyo yi-200μL, kwaye umthamo we-lution ocetyiswayo yi-100μL.
