I-Mycobacterium Tuberculosis ye-Isoniazid yokuNxhathisa uGuquko

I-Mycobacterium Tuberculosis Isoniazid Resistance Uguqulo Umfanekiso okhoyo

Inkcazelo emfutshane:

Le khithi ilungele ukuchongwa okusemgangathweni kweendawo eziphambili zokuguqula iisampulu zesikhohlela somntu eziqokelelwe kwizigulane ezine-Tubercle bacillus positive ezikhokelela kuxhathiso lwe-mycobacterium tuberculosis isoniazid: Ingingqi ye-InhA yokukhuthaza -15C>T, -8T>A, -8T>C;Ummandla womgqugquzeli we-AhpC -12C>T, -6G>A;ukuguqulwa kwe-homozygous ye-KatG 315 codon 315G>A, 315G>C.

Thumela i-imeyile kuthi

Iinkcukacha zeMveliso

Iithegi zeMveliso

Igama lemveliso

I-HWTS-RT137 I-Mycobacterium Tuberculosis I-Isoniazid i-Resistance Uguqulo lweKhithi yokuFundisa (i-Melting Curve)

I-Epidemiology

I-Mycobacterium tuberculosis, kungekudala njenge-Tubercle bacillus (TB), yibhaktiriya ebangela isifo sephepha.Okwangoku, amachiza alwa nesifo sephepha asetyenziswayo odidi lokuqala aquka isoniazid, rifampicin kunye nehexambutol, njl. Nangona kunjalo, ngenxa yokusetyenziswa gwenxa kwamachiza okulwa nesifo sephepha kunye neempawu zesakhiwo sodonga lweseli yesifo sephepha se-mycobacterium, isifo sephepha se-mycobacterium siphuhlisa ukuxhathisa ichiza kumachiza okulwa nesifo sephepha, ezisa imingeni enzima kuthintelo kunye nonyango lwesifo sephepha.

Umjelo

FAM	MP nucleic acid
ROX	Ulawulo lwangaphakathi

IiParameters zobuGcisa

Ugcino	≤-18℃
Beka ubomi kwishelufa	Iinyanga ezili-12
Uhlobo loMfanekiso	isikhohlela
CV	≤5%
LoD	Umda wokubona uhlobo lwasendle lwebhaktiriya ye-isoniazid exhathisayo yi-2x103 ibhaktiriya/mL, kwaye umda wokubhaqwa kwebhaktiriya eguqukayo yi-2x103 ibhaktiriya/mL.
Ukuchaza ngokuthe ngqo	a.Akukho kusabela okunqamlezileyo phakathi kwejenome yomntu, ezinye iintsholongwane ze-mycobacteria ezingekho ntuberculous kunye nenyumoniya ezifunyenwe yile khithi.b.Iziza zotshintsho zezinye iijini ezixhathisa ngamachiza kuhlobo lwasendle lweMycobacterium tuberculosis, ezifana nommandla omisela uxhathiso lwejini ye-rifampicin rpoB, zichongiwe, kwaye iziphumo zovavanyo azibonisi ukuxhathisa kwi-isoniazid, ezibonisa ukuba akukho kuphinda kusebenze kwakhona.
Izixhobo Ezisebenzayo	SLAN-96P Iinkqubo zePCR zeXesha langempelaIiNkqubo zePCR zeBioRad CFX96 zeXesha lokwenyani I-LightCycler480® Real-Time PCR System

Ukuhamba komsebenzi

Ukuba usebenzisa i-Macro & Micro-Test General DNA/RNA Kit (HWTS-3019) (enokuthi isetyenziswe kunye neMacro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) nguJiangsu Macro & Micro-Test I-Med-Tech Co., Ltd. yokukhutshwa, yongeza i-200μL yolawulo olungalunganga kunye nesampula yesikhohlela esenziweyo ukuba ivavanywe ngokulandelelana, kwaye yongeze i-10μL yolawulo lwangaphakathi ngokwahlukeneyo kulawulo olubi, isampulu yesikhohlela esetyenziweyo ukuba ivavanywe, kwaye amanyathelo alandelayo kufuneka enziwe ngokungqongqo ngokwemiyalelo yokutsalwa.Umthamo wesampulu ekhutshiweyo ngama-200μL, kunye nomthamo we-elution ocetyiswayo li-100μL.