Uguquko lweMycobacterium Tuberculosis INH
Igama lemveliso
Ikhithi yokuHlola utshintsho lweMycobacterium Tuberculosis INH (iMelting Curve) yeHWTS-RT137
I-Epidemiology
I-Mycobacterium tuberculosis, egama linguTubercle bacillus (TB) ngokufutshane, yintsholongwane ebangela isifo sephepha. Okwangoku, amayeza okuqala asetyenziswa kakhulu okulwa nesifo sephepha aquka i-INH, i-rifampicin kunye ne-hexambutol, njl. Amayeza amabini asetyenziswa kakhulu okulwa nesifo sephepha aquka i-fluoroquinolones, i-amikacin kunye ne-kanamycin, njl. Amayeza amatsha aphuhlisiwe yi-linezolid, i-bedaquiline kunye ne-delamani, njl. Nangona kunjalo, ngenxa yokusetyenziswa gwenxa kwamayeza okulwa nesifo sephepha kunye neempawu zesakhiwo sodonga lweseli lwe-mycobacterium tuberculosis, i-mycobacterium tuberculosis ikhula ukumelana namayeza okulwa nesifo sephepha, nto leyo ezisa imingeni enzima ekuthinteleni nasekunyangeni isifo sephepha.
Itshaneli
| I-FAM | i-asidi ye-nucleic ye-MP |
| I-ROX | Ulawulo lwangaphakathi |
Iiparameters zobugcisa
| Indawo yokugcina izinto | ≤-18℃ |
| Beka ubomi kwishelufa | Iinyanga ezili-12 |
| Uhlobo lweSibonelelo | isikhwehlela |
| CV | ≤5% |
| I-LoD | Umda wokufumanisa iintsholongwane ze-INH zohlobo lwasendle yi-2x103 bacteria/mL, kwaye umda wokufumanisa iintsholongwane eziguquliweyo yi-2x103 bacteria/mL. |
| Ukuchaneka | a. Akukho mpendulo idibeneyo phakathi kwe-genome yomntu, ezinye iintsholongwane ze-mycobacteria ezingenasifo sephepha kunye ne-pneumonia ezifunyenwe yile kit.b. Iindawo zotshintsho lwezinye iijini ezichasene namayeza kwisifo sephepha iMycobacterium esikwindawo yasendle, ezifana nommandla omisela ukumelana kwejini ye-rifampicin rpoB, zifunyenwe, kwaye iziphumo zovavanyo azibonisanga ukumelana ne-INH, nto leyo ebonisa ukuba akukho reactivity idibeneyo. |
| Izixhobo Ezisebenzayo | Iinkqubo ze-PCR ze-SLAN-96P zeXesha langempelaIinkqubo ze-PCR zeBioRad CFX96 zeXesha langempelaI-LightCycler480®Inkqubo ye-PCR yeXesha langempela |
Ukuhamba komsebenzi
Ukuba usebenzisa iMacro & Micro-Test General DNA/RNA Kit (HWTS-3019) (engasetyenziswa neMacro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) yiJiangsu Macro & Micro-Test Med-Tech Co., Ltd. ukuze kukhutshwe, yongeza i-200μL yesampulu ye-negative control kunye ne-processed sputum ukuze ivavanywe ngokulandelelana, kwaye yongeza i-10μL ye-internal control ngokwahlukeneyo kwisampulu ye-negative control, i-processed sputum eza kuvavanywa, kwaye amanyathelo alandelayo kufuneka enziwe ngokungqongqo ngokwemiyalelo yokukhupha. Umthamo wesampulu okhutshiweyo yi-200μL, kwaye umthamo wokucoca ocetyiswayo yi-100μL.
