I-Staphylococcus Aureus kunye ne-Methicillin-Resistant Staphylococcus Aureus (MRSA/SA)

Inkcazelo emfutshane:

Le khithi isetyenziselwa ukufunyaniswa komgangatho we-staphylococcus aureus kunye ne-methicillin-resistant staphylococcus aureus nucleic acids kwiisampulu zesikhohlela somntu, iisampulu ze-swab yeempumlo kunye nolusu kunye neesampuli zosulelo lwezicubu ezithambileyo kwi-vitro.


Iinkcukacha zeMveliso

Iithegi zeMveliso

Igama lemveliso

I-HWTS-OT062 i-Staphylococcus Aureus kunye ne-Methicillin-Resistant Staphylococcus Aureus (MRSA/SA) i-Nucleic Acid Detection Kit(Fluorescence PCR)

Isatifikethi

CE

I-Epidemiology

I-Staphylococcus aureus yenye yeebhaktheriya ezibalulekileyo ze-pathogenic zosulelo lwe-nosocomial.I-Staphylococcus aureus (SA) yeye-staphylococcus kwaye imele ibhaktheriya ye-Gram-positive, enokuvelisa iindidi ze-toxins kunye nee-enzymes ezihlaselayo.Iibhaktheriya zineempawu zokusabalalisa ngokubanzi, i-pathogenicity enamandla kunye nesantya esiphezulu sokumelana.I-nuclease gene ye-Thermostable (i-nuc) yimfuza egcinwe kakhulu ye-staphylococcus aureus.

Umjelo

FAM Uhlobo lofuzo lweMethicillin-resistant mecA
ROX

Ulawulo lwangaphakathi

CY5 staphylococcus aureus nuc gene

IiParameters zobuGcisa

Ugcino ≤-18℃ kwaye ikhuselwe ekukhanyeni
Beka ubomi kwishelufa Iinyanga ezili-12
Uhlobo loMfanekiso isikhohlela, ulusu kunye neesampulu zosulelo lwethishu ethambileyo, kunye neesampulu zokuswaya ngeempumlo
Ct ≤36
CV ≤5.0%
LoD I-1000 CFU / mL i-staphylococcus aureus, i-1000 CFU / mL ibhaktheriya echasene ne-methicillin.Xa ikhithi ibhaqa ireferensi ye-LoD yesizwe, i-1000/mL ye-staphylococcus aureus inokubhaqwa.
Ukuchaza ngokuthe ngqo Uvavanyo lwendawo yokuhlaselwa kwakhona lubonisa ukuba le kit ayinandawo yokusebenza kunye nezinye ezinye izinto zokuphefumla ezinjenge-meticillin-pugollococcus, eschesillin-enganyangiweyo I-Anriyi, iProtes mirabilis, enterobacter cloacae, streptococcus pneumoniae, enterococcus faecium, candida albicans, legionella pneumophila, candida parapsilosis, moraxella catarrhalis, neisseria meningitidis, haemophilus influenzae.
Izixhobo Ezisebenzayo Iinkqubo ze-Biosystems ezisetyenzisiweyo 7500 Iinkqubo zePCR zeXesha langempela

QuantStudio®IiNkqubo ze-PCR ze-5 zeXesha langempela

SLAN-96P Iinkqubo zePCR zeXesha langempela

I-LightCycler®Inkqubo ye-PCR ye-480 yexesha langempela

I-LineGene 9600 Plus Real-Time PCR Detection System

MA-6000 Real-Time Quantitative Thermal Cycler

I-BioRad CFX96 Inkqubo yePCR yexesha lokwenyani

BioRad CFX Opus 96 Real-Time PCR System

Ukuhamba komsebenzi

Ukhetho loku-1.

IMacro & Micro-Test Genomic DNA/RNA Kit (HWTS-3019) nguJiangsu Macro & Micro-Test Med-Tech Co., Ltd. ingasetyenziswa kunye neMacro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS- 3006B).Yongeza i-200µL ye-saline eqhelekileyo kwi-precipitate ehlaziyiweyo, kwaye amanyathelo alandelayo kufuneka atsalwe ngokwemiyalelo, kwaye umthamo we-elution ocetyiswayo yi-80µL.

Ukhetho lwesi-2.

I-Macro & Micro-Test Sample Release Reagent (HWTS-3005-8) yi-Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Yongeza i-1mL ye-saline eqhelekileyo kwi-precipitate emva kokuhlamba nge-saline eqhelekileyo, uze udibanise kakuhle.I-Centrifuge kwi-13,000r/min imizuzu emi-5, susa i-supernatant (gcina i-10-20µL ye-supernatant), kwaye ulandele imiyalelo yokutsalwa okulandelayo.

I-Reagent yokutsalwa ecetyiswayo: I-Nucleic Acid Extraction okanye i-Reagent yokucoca (YDP302) yi-Tiangen Biotech (Beijing) Co.,Ltd.Ukukhutshwa kufuneka kuqhutywe ngokungqongqo ngokwenyathelo lesi-2 lencwadana yomyalelo.Kuyacetyiswa ukuba kusetyenziswe i-RNase kunye ne-DNase-free water ku-elution kunye nomthamo we-100µL.

 


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