I-MyCobacterium Tuclotium tucleic acid kunye ne-rifampampin
Igama lemveliso
I-HWTS-RT074B-I-MyCobacterium Tucleic Subleid Acid kunye ne-Rifampin yokuCoca
Isatifiketi
CE
Isipikumu
Isifo se-mycobacterium, ngokufutshane njenge-tubercle bacils, i-TB, yibacterium ye-pathogenic ebangela i-tuberculosis. Okwangoku, amachiza asetyenziswayo e-Anti-Tuberculosis ruberculosis iquka i-isoniazid, i-rifampicin kunye ne-hexambuto yesifo se-tuoroquinol, njl. Njl. . Nangona kunjalo, ngenxa yokusetyenziswa okungachanekanga kwe-anti-tuberculosis Iziyobisi kunye neempawu zesakhiwo sodonga lweseli ze-Mycobacterium Tuberculosis, isifo sentloko ye-Mycobacterium sikhula ngokuchasene neziyobisi kumachiza asisifo se-Anti-Tuibsis, ozisa imiceli mngeni kutofotofo.
I-Rifampicin isetyenziswe ngokubanzi kunyango lwezigulana zesifo sephepha zokutsala ukusukela kwiminyaka yoo-1970, kwaye inesiphumo esibonakalayo. Kube kukhetho lokuqala ukunciphisa i-chemotherapy yezigulana zesifo sephepha Ukunganyangeki kweRifampicin kubangelwa ikakhulu kukuguqulwa kwe-RPEB Gene. Nangona amachiza amatsha e-Anti-Tuberculosis ihlala iphuma, kwaye ukusebenza kakuhle kwezigulana zesifo sephepha i-pulmonary tursodasis kuqhubeke nokuphucula iziyobisi ezichasene neziyobisi kwikliniki kuphakama. Ngokucacileyo, isifo se-mycobacterium kwizigulana esine-pullary tuberculosisy ayinakubulawa ngokupheleleyo ngexesha elithile, eliza kukhokelela kwizidanga ezahlukeneyo zeziyobisi, kwaye yonyusa umngcipheko wokufa kwesigulana.
Ijelo
| Ijelo | Iindlela kunye ne-fluorophores | Ukuphendula i-buffer a | Ukuphendula kwakhona buffer b | Ukuphendula i-buffer c |
| Itshaneli | Intatheli: Intsapho, iQuwenner: Akukho | I-RPOB 507-514 | I-RPOB 513-50 | I-38kd kwaye i-7110 |
| Itshaneli ye-cy5 | Intatheli: I-CY5, i-quenchar: akukho | I-RPOB 520-5577 | I-RPOB 527-533 | / |
| I-Hex (VIV) | Intatheli: Hex (VIV), i-quencir: akukho | Ulawulo lwangaphakathi | Ulawulo lwangaphakathi | Ulawulo lwangaphakathi |
Iiparamitha zobugcisa
| Ukugcinwa | ≤-18 ℃ ngobumnyama |
| Beka ubomi kwishelufa | Iinyanga ezili-12 |
| Uhlobo locaphu | Sputum |
| CV | ≤5.0% |
| Indawo yokuhlala | Isifo se-MyCobacterium Tuberculosis 50 i-bacteria / ml Uhlobo lwe-Rifampicin--undent Trand: 2x103I-bacteria / ml I-Homozygous iguqukayo: 2x103I-bacteria / ml |
| Ubuchule | Ibonanisa isifo sephepha se-mycobacterium ye-mycobacterium kunye neendawo eziguqukayo zenye i-Entersus enganyangekiyo njenge-KTG 315G> T the-Paistal> T, i-\-I-TER-15c |
| Izixhobo ezifanelekileyo: | I-Slan-96p yangempela ye-PCR ye-PCR I-biorad cfx96 yeyokwenyani ye-PFRR I-FIMCYCLERCER480® ye-Pow-Time-fast System |
Ukuhamba komsebenzi
Ukuba sebenzisa i-MACR & MIC-jikelele yeDNA / RNA Kit (HWTS-3019-50, I-HWTS-3019-3- 38, HWTS-3019-96) I-nucleic acid i-extractor (i-HWTS-3006C, HWTS-3006B) okanye i-Macro kunye ne-Macro kunye neRNA Ikholamu (i-HWTS-3022-50) nguJiangsu Macro & Viest Coul-Tex Countral Coul-Tensor Countration, yongeza i-2000l yesampulu, yongeza i-stoutum ye-stoutum ukuba ivavanywe, kwaye yongeza i-10μl ye-10μl ye Ulawulo lwangaphakathi ngokwahlukeneyo kulawulo olululo, ulawulo olubi kunye nesampulu ye-stoutum esebenzayo ukuba ihlolwe, kwaye amanyathelo alandelayo kufuneka ezenziwe ngokungqongqo ngokwemiyalelo yokukhuphela. Ivolumu yesampulu ekhutshiweyo yi-2007, kwaye ivolumu yesiqhelo ye-LELL yi-100μl.
