I-Mycobacterium Tuberculosis Nucleic Acid kunye neRifampicin Resistance
Igama lemveliso
I-HWTS-RT074B-Mycobacterium Tuberculosis Nucleic Acid kunye ne-Rifampicin Resistance Kit (iKit yokuNyibilika)
Isatifikethi
CE
I-Epidemiology
I-Mycobacterium tuberculosis , kungekudala njenge-Tubercle bacillus, i-TB, yintsholongwane ye-pathogenic ebangela isifo sephepha. Okwangoku, amachiza alwa nesifo sephepha asetyenziswa ngokuqhelekileyo kudidi lokuqala aquka isoniazid, rifampicin kunye nehexambutol, njl. Amachiza okulwa nesifo sephepha odidi lwesibini aquka i-fluoroquinolones, i-amikacin kunye ne-kanamycin, njl. isifo sephepha, mycobacterium tuberculosis iphuhlisa ukuxhathisa ngamachiza kumachiza achasene nesifo sephepha, nto leyo izisa imingeni enzima kuthintelo nakunyango lwesifo sephepha.
I-Rifampicin isetyenziswe ngokubanzi kunyango lwezigulane ze-pulmonary tuberculosis ukususela ekupheleni kwe-1970s, kwaye inesiphumo esibalulekileyo. Kuye kwaba yinto yokuqala yokukhetha ukunciphisa i-chemotherapy yezigulane ze-pulmonary tuberculosis. Ukumelana ne-Rifampicin kubangwa ikakhulu kukuguqulwa kwejini ye-rpoB. Nangona amachiza amatsha okulwa nesifo sephepha ehlala ephuma, kunye nokusebenza kwezonyango kwizigulana zesifo sephepha semiphunga nako kuqhubekile ukuphucuka, kusekho ukunqongophala kweziyobisi ezichasene nesifo sephepha, kwaye isenzeko sokusetyenziswa kweziyobisi ngokungekho ngqiqweni ekliniki siphezulu. Ngokucacileyo, isifo sephepha seMycobacterium kwizigulane ezine-pulmonary tuberculosis ayikwazi ukubulawa ngokupheleleyo ngexesha elifanelekileyo, ekugqibeleni likhokelela kwiinqanaba ezahlukeneyo zokunganyangeki kweziyobisi emzimbeni wesigulane, kwandisa ixesha lesi sifo, kwaye kwandisa umngcipheko wokufa kwesigulane.
Umjelo
| Umjelo | Imijelo kunye neFluorophores | Isithinteli sokuphendula A | Isithinteli sokuphendula B | Isithinteli sokuphendula C |
| FAM Channel | Intatheli: FAM, Quencher: Akukho | rpoB 507-514 | rpoB 513-520 | 38KD kunye ne-IS6110 |
| Ijelo le-CY5 | Intatheli: CY5, Quencher: Akukho | rpoB 520-527 | rpoB 527-533 | / |
| Ijelo le-HEX (VIC). | Intatheli: HEX (VIC), Quencher: Akukho | Ulawulo lwangaphakathi | Ulawulo lwangaphakathi | Ulawulo lwangaphakathi |
Iiparamitha zobuGcisa
| Ugcino | ≤-18℃ ebumnyameni |
| Beka ubomi kwishelufa | Iinyanga ezili-12 |
| Uhlobo loMfanekiso | Isikhohlela |
| CV | ≤5.0% |
| LoD | mycobacterium tuberculosis 50 bacteria/mL uhlobo lwasendle oluxhathisa i-rifampicin: 2x103ibhaktheriya/mL i-homozygous mutant: 2x103ibhaktheriya/mL |
| Ukuchaza ngokuthe ngqo | Ibhaqa i-mycobacterium tuberculosis yohlobo lwasendle kunye neziza zotshintsho zezinye iindlela zokumelana namachiza ezifana ne-katG 315G>C\A, InhA-15C> T, iziphumo zovavanyo zibonisa ukunganyangeki kwi-rifampicin, into ethetha ukuba akukho kuphinda-phinda kusebenze. |
| Izixhobo Ezisebenzayo: | SLAN-96P Iinkqubo zePCR zeXesha langempela I-BioRad CFX96 Inkqubo yePCR yexesha lokwenyani I-LightCycler480® Real-Time PCR System |
Ukuhamba komsebenzi
Ukuba usebenzisa i-Macro & Micro-Test General DNA/RNA Kit (HWTS-3019-50, HWTS-3019-32, HWTS-3019-48, HWTS-3019-96) (enokuthi isetyenziswe kunye neMacro & Micro-Test Automatic Nucleic Acid Extractor (HTSW60) HTSW30 (HTSW6) kunye ne-Micro-Test Viral DNA/RNA Column (HWTS-3022-50) yi-Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. ukuze kutsalwe, yongeza i-200μL yoLawulo olulungileyo, ulawulo olubi kunye nesampula yesikhohlela esicutshungulweyo ukuba sivavanywe ngokulandelelana, kwaye yongeza i-10μL yolawulo olubi lwenkqubo yolawulo lwangaphakathi kunye ne-positive controls yenkqubo yangaphakathi. kuvavanywa, kwaye amanyathelo alandelayo kufuneka aqhutywe ngokungqongqo ngokwemiyalelo yokutsalwa. Umthamo wesampulu ekhutshiweyo yi-200μL, kwaye umthamo we-lution ocetyiswayo yi-100μL.
