Mycoplasma hominis, Ureaplasma urealyticum kunye ne Gardnerella vaginalis Nucleic Acid
Igama lemveliso
I-HWTS-UR044-Mycoplasma hominis, i-Ureaplasma urealyticum kunye ne-Gardnerella vaginalis Nucleic Acid Detection Kit (i-Fluorescence PCR)
I-Epidemiology
I-Mycoplasma hominis (MH) luhlobo lwe-mycoplasma olufumaneka kwindlela yomchamo nakwizitho zangasese kwaye lunokubangela usulelo lwendlela yomchamo kunye nokudumba kwesini. I-Mycoplasma hominis ikhona kakhulu kwindalo kwaye inxulunyaniswa neentlobo ngeentlobo zosulelo lwendlela ye-genitourinary ezifana ne-nongonococcal urethritis, i-cervicitis yabasetyhini, i-adnexitis, ukungazali, njl. I-Ureaplasma urealyticum (UU) yeyona microorganism incinci ye-prokaryotic celled ephakathi kweebhaktheriya kunye neentsholongwane kwaye inokuphila ngokuzimela, kwaye ikwayi-microorganism ebangela ngokulula usulelo lwendlela yokuzala kunye nendlela yomchamo. Kumadoda, inokubangela i-prostatitis, i-urethritis, i-pyelonephritis, njl.njl.; kubasetyhini, inokubangela iimpendulo zokudumba kwindlela yokuzala efana ne-vaginitis, i-cervicitis, kunye nesifo sokudumba kwesinqe, kwaye yenye yezifo ezibangela ukungazali kunye nokuphuphuma kwesisu. Eyona nto ixhaphakileyo ye-vaginitis kubasetyhini yi-bacterial vaginosis, kwaye i-bacterial pathogenic ebalulekileyo ye-bacterial vaginosis yiGardnerella vaginalis. I-Gardnerella vaginalis (GV) yintsholongwane enobungozi engabangeli zifo xa ikhona ngobuninzi obuncinci. Nangona kunjalo, xa iintsholongwane ezilawulayo zesini i-Lactobacilli zincitshiswa okanye zipheliswe, nto leyo ebangela ukungalingani kwindawo yesini, i-Gardnerella vaginalis iyanda ngamanani amakhulu, nto leyo ekhokelela kwi-bacterial vaginosis. Kwangaxeshanye, ezinye iintsholongwane (ezifana ne-Candida, i-Neisseria gonorrhoeae, i-Mycoplasma hominis, njl.njl.) zinokungena emzimbeni womntu, zibangele i-mixed vaginitis kunye ne-cervicitis. Ukuba i-vaginitis kunye ne-cervicitis azichongwa kwaye azinyangwa ngexesha elifanelekileyo, kunokubakho izifo ezikhulayo ezibangelwa ziintsholongwane ezikwi-mucosa yendlela yokuzala, nto leyo ekhokelela ngokulula kwizifo eziphezulu zesini ezifana ne-endometritis, i-salpingitis, i-tubo-ovarian abscess (TOA), kunye ne-pelvic peritonitis, nto leyo enokubangela iingxaki ezinkulu ezifana nokungazali, ukukhulelwa okungaqhelekanga kunye neziphumo ezimbi zokukhulelwa.
Iiparameters zobugcisa
| Indawo yokugcina izinto | ≤-18℃ |
| Beka ubomi kwishelufa | Iinyanga ezili-12 |
| Uhlobo lweSibonelelo | iswabhu ye-urethral yamadoda, iswabhu ye-cervical yabasetyhini, iswabhu ye-vaginal yabasetyhini |
| Ct | ≤38 |
| CV | ＜5.0% |
| I-LoD | I-UU, i-GV 400Copies/mL; I-MH 1000Copies/mL |
| Izixhobo Ezisebenzayo | Isebenza kwi-type I detection reagent: Iinkqubo ze-PCR ze-Applied Biosystems 7500 Real-Time, I-QuantStudio®Iinkqubo ezi-5 ze-PCR zeXesha langempela, Iinkqubo ze-PCR ze-SLAN-96P zeXesha langempela (iHongshi Medical Technology Co., Ltd.), Iinkqubo zokuFumanisa i-LineGene 9600 Plus Real-Time PCR (FQD-96A, iteknoloji ye-Hangzhou Bioer), I-MA-6000 Real-Time Quantitative Thermal Cycler (iSuzhou Molarray Co., Ltd.), Inkqubo ye-PCR ye-BioRad CFX96 yeXesha langempela, Inkqubo ye-PCR ye-BioRad CFX Opus 96 yeXesha langempela. Isebenza kwi-reagent yokuchonga uhlobo lwesibini: U-EudemonTMI-AIO800 (HWTS-EQ007) yiJiangsu Macro kunye neMicro-Test Med-Tech Co., Ltd. |
Ukuhamba komsebenzi
I-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (engasetyenziswa kunye ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)), kunye ne-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-8) (engasetyenziswa kunye ne-EudemonTM I-AIO800 (HWTS-EQ007)) yiJiangsu Macro kunye neMicro-Test Med-Tech Co., Ltd.
Umthamo wesampulu ekhutshiweyo yi-200μL kwaye umthamo we-elution ocetyiswayo yi-150μL.
