I-Mycoplasma Pneumoniae (MP)
Igama lemveliso
I-HWTS-RT024 Mycoplasma Pneumoniae (MP) yeNucleic Acid Detection Kit(Fluorescence PCR)
I-Epidemiology
I-Mycoplasma pneumoniae (MP) luhlobo lweyona microorganism encinci yeprokaryotic, ephakathi kwebhaktheriya kunye nentsholongwane, kunye nesakhiwo seseli kodwa akukho ludonga lweseli.I-MP ikakhulu ibangela usulelo lwendlela yokuphefumla yabantu, ngakumbi ebantwaneni nakubantu abancinci.Inokubangela inyumoniya ye-mycoplasma yabantu, usulelo lwendlela yokuphefumla yabantwana kunye nenyumoniya engaqhelekanga.Ukubonakaliswa kweklinikhi kuyahluka, uninzi lwazo lukhohlokhohlo olunzima, umkhuhlane, ukugodola, intloko ebuhlungu, umqala obuhlungu.Ukosuleleka kwendlela yokuphefumula ephezulu kunye nenyumoniya yebronchial zezona zixhaphakileyo.Ezinye izigulana zinokukhula ukusuka kusulelo lwendlela yokuphefumla ephezulu ukuya kwinyumoniya ebukhali, uxinzelelo olukhulu lokuphefumla kunye nokufa kungenzeka.
Umjelo
| FAM | Mycoplasma pneumoniae |
| VIC/HEX | Ulawulo lwangaphakathi |
IiParameters zobuGcisa
| Ugcino | ≤-18℃ |
| Beka ubomi kwishelufa | Iinyanga ezili-12 |
| Uhlobo loMfanekiso | Isikhohlela, i-Oropharyngeal swab |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | Iikopi ezingama-200/mL |
| Ukuchaza ngokuthe ngqo | a） Ukuphinda kusebenze kwakhona okunqamlezileyo: akukho kuphinda kusebenze kunye ne-Ureaplasma urealyticum, Mycoplasma genitalium, Mycoplasma hominis, Streptococcus pneumoniae, Chlamydia pneumoniae, Haemophilus influenzae, Klebsiella pneumoniae, Staphylococcus aureus, Mycobacteriuuinodocuum aphilamonaella, Leycobacteriuinodococcus aphilamonaella ibhaktheriya baumannii, intsholongwane yoMkhuhlane A , Intsholongwane ye-Influenza B, i-Parainfluenza virus uhlobo lwe-I / II / III / IV, i-Rhinovirus, i-Adenovirus, i-Metapneumovirus yabantu, i-Respiratory syncytial virus kunye ne-genomic nucleic acid yabantu. b）Amandla okuchasana nokuphazanyiswa: akukho kuphazamiseka xa izinto eziphazamisayo zivavanywa ngezigxina zilandelayo: i-hemoglobin (50mg / L), i-bilirubin (20mg / dL), i-mucin (60mg / mL), i-10% (v / v) igazi lomntu, levofloxacin (10μg/mL), moxifloxacin (0.1g/L), gemifloxacin (80μg/mL), azithromycin (1mg/mL), clarithromycin (125μg/mL), erythromycin (0.5g/L), doxycycline (50mg) / L), i-minocycline (0.1g/L). |
| Izixhobo Ezisebenzayo | Isetyenziselwe i-Biosystems ye-7500 ye-Real-Time PCR System Iinkqubo ze-Biosystems eziSetyenzisiweyo 7500 Iinkqubo zePCR ezikhawulezayo QuantStudio®IiNkqubo ze-PCR ze-5 zeXesha langempela SLAN-96P Iinkqubo zePCR zeXesha lokwenyani (Hongshi Medical Technology Co., Ltd.) I-LightCycler®Inkqubo ye-PCR ye-480 yexesha langempela I-LineGene 9600 Plus ye-Real-Time PCR Detection System (FQD-96A, iteknoloji ye-Hangzhou Bioer) I-MA-6000 yeXesha lokwenyani iBhayisekile yoMyinge weThermal (iSuzhou Molarray Co., Ltd.) I-BioRad CFX96 Inkqubo yePCR yexesha lokwenyani BioRad CFX Opus 96 Real-Time PCR System |
Ukuhamba komsebenzi
(1) Isampulu yesikhohlela
I-reagent yokutsalwa ecetyiswayo: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (enokuthi isetyenziswe kunye neMacro & Micro-Test I-Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) yi-Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Yongeza i-200µL ye-saline eqhelekileyo kwi-precipitate ehlaziyiweyo.Ukukhutshwa okulandelayo kufuneka kwenziwe ngokomyalelo wokusetyenziswa.Umthamo we-elution ocetyiswayo yi-80µL.Isixhobo sokutsalwa esicetyiswayo: Ukutsalwa kwe-Nucleic Acid okanye iReagent yokuCoca (YDP315-R).Ukukhutshwa kufuneka kwenziwe ngokungqongqo ngokomyalelo wokusetyenziswa.Umthamo we-elution ocetyiswayo yi-60µL.
(2) I-Oropharyngeal swab
I-reagent yokutsalwa ecetyiswayo: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (enokuthi isetyenziswe kunye neMacro & Micro-Test I-Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) nguJiangsu Macro & Micro-Test Med-Tech Co., Ltd. Ukukhutshwa kufuneka kwenziwe ngokomyalelo wokusetyenziswa.Umthamo wotsalo ocetyiswayo wesampulu yi-200µL, kunye nomthamo ocetyiswayo we-elution yi-80µL.Isixhobo sokukhutshwa esicetyiswayo: QIAamp Viral RNA Mini Kit (52904) okanye iNucleic Acid Extraction okanye i-Purification Reagent (YDP315-R).Ukukhutshwa kufuneka kwenziwe ngokungqongqo ngokomyalelo wokusetyenziswa.Umthamo wokutsalwa ocetyiswayo wesampulu yi-140µL, kwaye umthamo we-elution ocetyiswayo yi-60µL.
