I-Mycoplasma Pneumoniae (MP)

Umfanekiso oPhambili weMycoplasma Pneumoniae (MP)

Inkcazo emfutshane:

Le mveliso isetyenziselwa ukufumanisa i-Mycoplasma pneumoniae (MP) nucleic acid kwisampuli ze-sputum yomntu kunye ne-oropharyngeal swab.

Thumela i-imeyile kuthi

Iinkcukacha zeMveliso

Iithegi zeMveliso

Igama lemveliso

Ikhithi yokuFumanisa i-HWTS-RT024 Mycoplasma Pneumoniae（MP） Nucleic Acid Detection (Fluorescence PCR)

I-Epidemiology

I-Mycoplasma pneumoniae (MP) luhlobo lwe-microorganism encinci ye-prokaryotic, ephakathi kwebhaktheriya kunye nentsholongwane, enesakhiwo seseli kodwa ingenaludonga lweseli. I-MP ikakhulu ibangela usulelo lwendlela yokuphefumla yabantu, ngakumbi kubantwana nakubantu abancinci. Ingabangela i-mycoplasma pneumonia yabantu, usulelo lwendlela yokuphefumla yabantwana kunye ne-pneumonia engaqhelekanga. Iimpawu zeklinikhi zahlukile, uninzi lwazo kukukhwehlela okukhulu, umkhuhlane, ukubanda, intloko ebuhlungu, umphimbo obuhlungu. Usulelo lwendlela yokuphefumla ephezulu kunye ne-bronchial pneumonia zezona zixhaphakileyo. Ezinye izigulana zinokuvela kusulelo lwendlela yokuphefumla ephezulu ukuya kwi-pneumonia enzima, uxinzelelo olukhulu lokuphefumla kunye nokufa kunokwenzeka.

Itshaneli

I-FAM	I-Mycoplasma pneumoniae
I-VIC/HEX	Ulawulo lwangaphakathi

Iiparameters zobugcisa

Indawo yokugcina izinto	≤-18℃
Beka ubomi kwishelufa	Iinyanga ezili-12
Uhlobo lweSibonelelo	I-sputum, i-oropharyngeal swab
Ct	≤38
CV	≤5.0%
I-LoD	Iikopi ezingama-200/mL
Ukuchaneka	a） Ukusabela okunqamlezileyo: akukho kusabela okunqamlezileyo kunye ne-Ureaplasma urealyticum, i-Mycoplasma genitalium, i-Mycoplasma hominis, i-Streptococcus pneumoniae, i-Chlamydia pneumoniae, i-Haemophilus influenzae, i-Klebsiella pneumoniae, i-Staphylococcus aureus, i-Mycobacterium tuberculosis, i-Legionella pneumophila, i-Pseudomonas aeruginosa, i-Acinetobacter baumannii, i-Influenza A virus, i-Influenza B virus, i-Parainfluenza virus type I/II/III/IV, i-Rhinovirus, i-Adenovirus, i-Human metapneumovirus, i-Respiratory syncytial virus kunye ne-human genomic nucleic acid. b）Ubuchule bokulwa nokuphazamiseka: akukho kuphazamiseka xa izinto eziphazamisayo zivavanyiwe ngala manqanaba alandelayo: i-hemoglobin (50mg/L), i-bilirubin (20mg/dL), i-mucin (60mg/mL), i-10% (v/v) igazi lomntu, i-levofloxacin (10μg/mL), i-moxifloxacin (0.1g/L), i-gemifloxacin (80μg/mL), i-azithromycin (1mg/mL), i-clarithromycin (125μg/mL), i-erythromycin (0.5g/L), i-doxycycline (50mg/L), i-minocycline (0.1g/L).
Izixhobo Ezisebenzayo	Inkqubo ye-PCR ye-Applied Biosystems 7500 Real-Time Iinkqubo ze-PCR eziSebenzisiweyo ze-7500 ezikhawulezayo zeXesha langempela I-QuantStudio^®Iinkqubo ezi-5 ze-PCR zeXesha langempela Iinkqubo ze-PCR ze-SLAN-96P zeXesha langempela (iHongshi Medical Technology Co., Ltd.) I-LightCycler^®Inkqubo ye-PCR yeXesha langempela ye-480 Inkqubo yokuFumanisa i-LineGene 9600 Plus yeXesha langempela le-PCR (i-FQD-96A, iteknoloji ye-Hangzhou Bioer) I-MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) Inkqubo ye-PCR ye-BioRad CFX96 yeXesha langempela Inkqubo ye-PCR ye-BioRad CFX Opus 96 yeXesha langempela

Ukuhamba komsebenzi

(1) Isampuli yesikhohlela

I-reagent yokukhupha ecetyiswayo: I-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (engasetyenziswa ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) yiJiangsu Macro & Micro-Test Med-Tech Co., Ltd. Yongeza i-200µL ye-saline eqhelekileyo kwi-precipitate ecutshungulwayo. Ukukhupha okulandelayo kufuneka kwenziwe ngokwemiyalelo yokusetyenziswa. Umthamo wokucoca ocetyiswayo yi-80µL. I-reagent yokucoca ecetyiswayo: I-Nucleic Acid Extraction okanye i-Purification Reagent (YDP315-R). Ukukhupha kufuneka kwenziwe ngokungqongqo ngokwemiyalelo yokusetyenziswa. Umthamo wokucoca ocetyiswayo yi-60µL.

(2) I-Oropharyngeal swab

I-reagent yokukhupha ecetyiswayo: I-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (engasetyenziswa ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) yiJiangsu Macro & Micro-Test Med-Tech Co., Ltd. Ukukhupha kufuneka kwenziwe ngokwemiyalelo yokusetyenziswa. Umthamo wokukhupha ocetyiswayo wesampulu yi-200µL, kwaye umthamo wokucoca ocetyiswayo yi-80µL. I-reagent yokukhupha ecetyiswayo: I-QIAamp Viral RNA Mini Kit (52904) okanye i-Nucleic Acid Extraction okanye i-Purification Reagent (YDP315-R). Ukukhupha kufuneka kwenziwe ngokungqongqo ngokwemiyalelo yokusetyenziswa. Umthamo ocetyiswayo wokukhupha isampuli yi-140µL, kwaye umthamo ocetyiswayo wokukhupha isampuli yi-60µL.

Ngaphambili: I-Clostridium difficile toxin A/B gene (C.diff)

Okulandelayo: I-Klebsiella Pneumoniae, i-Acinetobacter Baumannii kunye ne-Pseudomonas Aeruginosa kunye ne-Drug Resistance Genes (KPC, NDM, OXA48 kunye ne-IMP) Multiplex

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