I-Mycoplasma Pneumoniae (MP)
Igama lemveliso
I-HWTS-RT024 Mycoplasma Pneumoniae (MP) yeNucleic Acid Detection Kit(Fluorescence PCR)
I-Epidemiology
I-Mycoplasma pneumoniae (MP) luhlobo lweyona microorganism encinci yeprokaryotic, ephakathi kwebhaktheriya kunye nentsholongwane, kunye nesakhiwo seseli kodwa akukho ludonga lweseli. I-MP ikakhulu ibangela usulelo lwendlela yokuphefumla yabantu, ngakumbi ebantwaneni nakubantu abancinci. Inokubangela inyumoniya ye-mycoplasma yabantu, usulelo lwendlela yokuphefumla yabantwana kunye nenyumoniya engaqhelekanga. Ukubonakaliswa kweklinikhi kuyahluka, uninzi lwazo lukhohlokhohlo olunzima, umkhuhlane, ukugodola, intloko ebuhlungu, umqala obuhlungu. Ukosuleleka kwendlela yokuphefumula ephezulu kunye nenyumoniya yebronchial zezona zixhaphakileyo. Ezinye izigulana zinokukhula ukusuka kusulelo lwendlela yokuphefumla ephezulu ukuya kwinyumoniya ebukhali, uxinzelelo olukhulu lokuphefumla kunye nokufa kungenzeka.
Umjelo
| FAM | Mycoplasma pneumoniae |
| VIC/HEX | Ulawulo lwangaphakathi |
Iiparamitha zobuGcisa
| Ugcino | ≤-18℃ |
| Beka ubomi kwishelufa | Iinyanga ezili-12 |
| Uhlobo loMfanekiso | Isikhohlela, i-Oropharyngeal swab |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | Iikopi ezingama-200/mL |
| Ukuchaza ngokuthe ngqo | a） Ukuphinda kusebenze kwakhona okunqamlezayo: akukho kuphinda kusebenze kunye ne-Ureaplasma urealyticum, Mycoplasma genitalium, Mycoplasma hominis, Streptococcus pneumoniae, Chlamydia pneumoniae, Haemophilus influenzae, Klebsiella pneumoniae, Staphylococcus aureus, Mycobacteriudoudomonolas i-aeruginosa, i-Acinetobacter baumannii, i-influenza A virus, i-virus ye-Influenza B, i-Parainfluenza uhlobo lwentsholongwane ye-I/II/III/IV, i-Rhinovirus, i-Adenovirus, i-Human metapneumovirus, i-Respiratory syncytial virus kunye ne-nucleic acid yabantu. b）Amandla okuchasana nokuphazanyiswa: akukho kuphazamiseka xa izinto eziphazamisayo zivavanywa ngezigxina zilandelayo: i-hemoglobin (50mg / L), i-bilirubin (20mg / dL), i-mucin (60mg / mL), i-10% (v / v) igazi lomntu, i-levofloxacin (10μg / mL), i-moxifloxacin / 8mL), i-moxifloxacin / 0. i-azithromycin (1mg / mL), i-clarithromycin (125μg / mL), i-erythromycin (0.5g / L), i-doxycycline (50mg / L), i-minocycline (0.1g / L). |
| Izixhobo Ezisebenzayo | Isetyenziselwe i-Biosystems ye-7500 ye-Real-Time PCR System Iinkqubo ze-Biosystems eziSetyenzisiweyo 7500 Iinkqubo zePCR ezikhawulezayo QuantStudio®IiNkqubo ze-PCR ze-5 zeXesha langempela SLAN-96P Iinkqubo zePCR zeXesha lokwenyani (Hongshi Medical Technology Co., Ltd.) I-LightCycler®Inkqubo ye-PCR ye-480 yexesha langempela I-LineGene 9600 Plus ye-Real-Time PCR Detection System (FQD-96A, iteknoloji ye-Hangzhou Bioer) I-MA-6000 yeXesha lokwenyani iBhayisekile yoMyinge weThermal (iSuzhou Molarray Co., Ltd.) I-BioRad CFX96 Inkqubo yePCR yexesha lokwenyani BioRad CFX Opus 96 Real-Time PCR System |
Ukuhamba komsebenzi
(1) Isampulu yesikhohlela
I-reagent yokutsalwa ecetyiswayo: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (enokuthi isetyenziswe kunye neMacro & Micro-Test Automatic Nucleic Acid (006W) HWTS-3006B)) nguJiangsu Macro kunye neMicro-Test Med-Tech Co., Ltd. Yongeza i-200µL yetyuwa eqhelekileyo kumsinga ogqityiweyo. Ukukhutshwa okulandelayo kufuneka kwenziwe ngokomyalelo wokusetyenziswa. Umthamo we-elution ocetyiswayo yi-80µL.Isixhobo sokutsalwa esicetyiswayo: Ukutsalwa kwe-Nucleic Acid okanye iReagent yokuCoca (YDP315-R). Ukukhutshwa kufuneka kwenziwe ngokungqongqo ngokomyalelo wokusetyenziswa. Umthamo we-elution ocetyiswayo yi-60µL.
(2) I-Oropharyngeal swab
I-reagent yokutsalwa ecetyiswayo: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (enokuthi isetyenziswe kunye neMacro & Micro-Test Automatic Nucleic Acid (006W) HWTS-3006B)) nguJiangsu Macro & Micro-Test Med-Tech Co., Ltd. Ukukhutshwa kufuneka kwenziwe ngokomyalelo wokusetyenziswa. Umthamo wokutsalwa ocetyiswayo wesampulu yi-200µL, kunye nomthamo we-elution ocetyiswayo yi-80µL.Isixhobo sokukhutshwa esicetyiswayo: QIAamp Viral RNA Mini Kit (52904) okanye iNucleic Acid Extraction okanye i-Purification Reagent (YDP315-R). Ukukhutshwa kufuneka kwenziwe ngokungqongqo ngokomyalelo wokusetyenziswa. Umthamo wokutsalwa ocetyiswayo wesampulu yi-140µL, kwaye umthamo we-elution ocetyiswayo yi-60µL.
