Ingcamango yethumba
Ithumba sisidalwa esitsha esenziwe kukwanda okungaqhelekanga kweeseli emzimbeni, okuhlala kubonakala njengobunzima bezicubu ezingaqhelekanga (iqhubu) kwindawo yomzimba. Ukwakheka kwethumba sisiphumo sokuphazamiseka okukhulu kokulawulwa kokukhula kweeseli phantsi kwesenzo sezinto ezahlukeneyo ezibangela ithumba. Ukwanda okungaqhelekanga kweeseli okukhokelela ekwakhiweni kwethumba kubizwa ngokuba kukwanda kwe-neoplastic.
Ngo-2019, iCancer Cell ipapashe inqaku kutshanje. Abaphandi bafumanise ukuba i-metformin inokuthintela kakhulu ukukhula kwethumba xa umntu ezila ukutya, kwaye bacebisa ukuba indlela ye-PP2A-GSK3β-MCL-1 isenokuba yeyona nto intsha esetyenziselwa unyango lwethumba.
Umahluko ophambili phakathi kwe-tumor engeyongozi kunye ne-tumor enobungozi
Ithumba elingeyongozi: ukukhula kancinci, ikhaphukhaphu, ukukhula okudumbayo, ukutyibilika xa uchukumisa, umda ocacileyo, akukho metastasis, i-prognosis elungileyo ngokubanzi, iimpawu zoxinzelelo lwengingqi, ngokubanzi akukho mzimba upheleleyo, ngesiqhelo akubangeli ukufa kwabaguli.
Ithumba elibi (umhlaza): ukukhula ngokukhawuleza, ukukhula okungafunekiyo, ukunamathela kwizicwili ezingqongileyo, ukungakwazi ukuhamba xa uchukunyiswa, umda ongacacanga, ukusasazeka ngokulula kwesifo, ukuphinda kube lula emva konyango, umkhuhlane ophantsi, ukungatyi ukutya kwasekuqaleni, ukwehla kobunzima, ukubhitya okukhulu, i-anemia kunye nomkhuhlane kwinqanaba lokugqibela, njl. Ukuba ayinyangwa ngexesha, idla ngokukhokelela ekufeni.
"Ngenxa yokuba izimila ezingenobungozi kunye nezimila ezinobungozi azinazo nje kuphela iimpawu ezahlukeneyo zeklinikhi, kodwa okubaluleke ngakumbi, isiphumo sazo sahlukile, ngoko ke wakuba ufumene iqhuma emzimbeni wakho kunye neempawu ezingentla, kufuneka ufune ingcebiso kagqirha kwangethuba."
Unyango lomntu ngamnye lwethumba
Iprojekthi yeGenome yabantu kunye neProjekthi yeGenome yoMhlaza yeHlabathi
Iprojekthi yeHuman Genome, eyasungulwa ngokusesikweni eMelika ngo-1990, ijolise ekuvuleni zonke iikhowudi zezakhi zofuzo ezimalunga ne-100,000 emzimbeni womntu nokuzoba uluhlu lwezakhi zofuzo zomntu.
Ngowama-2006, iProjekthi yeGenome yoMhlaza yeHlabathi, eyaqaliswa ngokudibeneyo ngamazwe amaninzi, lolunye uphando olukhulu lwesayensi emva kweProjekthi yeGenome yoLuntu.
Iingxaki eziphambili kunyango lwe-tumor
Ukuxilongwa komntu ngamnye kunye nonyango = Ukuxilongwa komntu ngamnye+amayeza ajoliswe kuwo
Kwizigulane ezininzi ezahlukeneyo ezinesifo esifanayo, indlela yonyango kukusebenzisa amayeza afanayo kunye nomlinganiselo oqhelekileyo, kodwa eneneni, izigulane ezahlukeneyo zinemiphumo eyahlukileyo kakhulu kwimiphumo yonyango kunye neempendulo ezimbi, kwaye ngamanye amaxesha lo mahluko ude ubulale.
Unyango olujoliswe kumayeza luneempawu zokubulala iiseli zethumba ngokukhetha kakhulu ngaphandle kokubulala okanye ukonakalisa iiseli eziqhelekileyo ngokungaqhelekanga, kunye neziphumo ebezingalindelekanga ezincinci, eziphucula ngokufanelekileyo umgangatho wobomi kunye nefuthe lonyango lwezigulane.
Ngenxa yokuba unyango olujoliswe kulo luyilelwe ukuhlasela iimolekyuli ezithile ekujoliswe kuzo, kuyimfuneko ukufumanisa iijini zethumba nokufumanisa ukuba izigulana zineethagethi ezifanelekileyo na ngaphambi kokuba zithathe amayeza, ukuze zisebenzise impembelelo yazo yokunyanga.
Ukufunyanwa kwezakhi zofuzo zethumba
Ukuchonga iijini zethumba yindlela yokuhlalutya nokulandelelanisa i-DNA/RNA yeeseli zethumba.
Ukubaluleka kokufunyanwa kwezakhi zofuzo zethumba kukukhokela ukukhethwa kwamayeza onyango (amayeza ajolisiweyo, izithinteli zokujonga amajoni omzimba kunye nezinye iintsholongwane ezintsha ze-AIDS, unyango olusexesheni), kunye nokuqikelela ukubikezela kwangaphambili kunye nokubuyela kwakhona kwesifo.
Izisombululo ezibonelelwe yi-Acer Macro kunye ne-Micro-Test
Ikhithi yokuFumanisa utshintsho lwe-EGFR Gene 29 yomntu (i-Fluorescence PCR)）
Isetyenziselwa ukufumanisa utshintsho oluqhelekileyo kwi-exon 18-21 ye-EGFR gene kubantu abanesifo somhlaza wemiphunga esingesiso iseli encinci kwi-vitro.
1. Ukuqaliswa kolawulo lomgangatho wesalathiso sangaphakathi kwinkqubo kunokubeka esweni ngokupheleleyo inkqubo yovavanyo nokuqinisekisa umgangatho wovavanyo.
2. Uvakalelo oluphezulu: izinga lokuguquka kwe-1% lingafunyanwa ngokuzinzileyo kwisisombululo se-3ng/μL se-wild-type nucleic acid reaction solution.
3. Ukuchaneka okuphezulu: akukho mpendulo ihambelanayo neziphumo zokufunyanwa kwe-DNA ye-genomic yomntu yohlobo lwasendle kunye nezinye iintlobo ze-mutant.
Ikhithi yokuFumanisa utshintsho lwe-KRAS 8 (i-Fluorescence PCR)
Iintlobo ezisibhozo zenguqu kwiikhodon 12 kunye ne-13 ze-K-ras gene ezisetyenziselwa ukufumanisa i-DNA ekhutshwe kwiindawo ze-pathological ezifakwe kwi-paraffin yomntu kwi-vitro.
1. Ukuqaliswa kolawulo lomgangatho wesalathiso sangaphakathi kwinkqubo kunokubeka esweni ngokupheleleyo inkqubo yovavanyo nokuqinisekisa umgangatho wovavanyo.
2. Uvakalelo oluphezulu: izinga lokuguquka kwe-1% lingafunyanwa ngokuzinzileyo kwisisombululo se-3ng/μL se-wild-type nucleic acid reaction solution.
3. Ukuchaneka okuphezulu: akukho mpendulo ihambelanayo neziphumo zokufunyanwa kwe-DNA ye-genomic yomntu yohlobo lwasendle kunye nezinye iintlobo ze-mutant.
Ikhithi yokuFumana uTshintsho lweZizalo ze-Human ROS1 (i-Fluorescence PCR)
Isetyenziselwa ukubona ngokwemigangatho iintlobo ezili-14 ze-mutation ze-ROS1 fusion gene kwizigulana zomhlaza wemiphunga ezingezizo iiseli ezincinci zabantu kwi-vitro.
1. Ukuqaliswa kolawulo lomgangatho wesalathiso sangaphakathi kwinkqubo kunokubeka esweni ngokupheleleyo inkqubo yovavanyo nokuqinisekisa umgangatho wovavanyo.
2. Uvakalelo oluphezulu: iikopi ezingama-20 ze-fusion mutation.
3. Ukuchaneka okuphezulu: akukho mpendulo ihambelanayo neziphumo zokufunyanwa kwe-DNA ye-genomic yomntu yohlobo lwasendle kunye nezinye iintlobo ze-mutant.
Ikhithi yokuFumana uTshintsho lweMfuzo ye-Human EML4-ALK (i-Fluorescence PCR)
Isetyenziselwa ukubona ngokwemigangatho iintlobo ezili-12 zotshintsho lwe-EML4-ALK fusion gene kwizigulana zomhlaza wemiphunga ezingezizo iiseli ezincinci zabantu kwi-vitro.
1. Ukuqaliswa kolawulo lomgangatho wesalathiso sangaphakathi kwinkqubo kunokubeka esweni ngokupheleleyo inkqubo yovavanyo nokuqinisekisa umgangatho wovavanyo.
2. Uvakalelo oluphezulu: iikopi ezingama-20 ze-fusion mutation.
3. Ukuchaneka okuphezulu: akukho mpendulo ihambelanayo neziphumo zokufunyanwa kwe-DNA ye-genomic yomntu yohlobo lwasendle kunye nezinye iintlobo ze-mutant.
Ikhithi yokuFumanisa uTshintsho lweGene V600E yeBreathe (iFluorescence PCR)
Isetyenziselwa ukubona ngokwemigangatho utshintsho lwe-BRAF gene V600E kwiisampuli zezicubu ezifakwe kwiparafini ze-melanoma yomntu, umhlaza we-colorectal, umhlaza we-thyroid kunye nomhlaza wemiphunga kwi-vitro.
1. Ukuqaliswa kolawulo lomgangatho wesalathiso sangaphakathi kwinkqubo kunokubeka esweni ngokupheleleyo inkqubo yovavanyo nokuqinisekisa umgangatho wovavanyo.
2. Uvakalelo oluphezulu: izinga lokuguquka kwe-1% lingafunyanwa ngokuzinzileyo kwisisombululo se-3ng/μL se-wild-type nucleic acid reaction solution.
3. Ukuchaneka okuphezulu: akukho mpendulo ihambelanayo neziphumo zokufunyanwa kwe-DNA ye-genomic yomntu yohlobo lwasendle kunye nezinye iintlobo ze-mutant.
| Inombolo yento | Igama lemveliso | Inkcazo |
| I-HWTS-TM006 | Ikhithi yokuFumana uTshintsho lweMfuzo ye-Human EML4-ALK (i-Fluorescence PCR) | Uvavanyo oluyi-20/ikhithi Uvavanyo oluyi-50/ikhithi |
| I-HWTS-TM007 | Ikhithi yokuFumanisa uTshintsho lweGene V600E yeBreathe (iFluorescence PCR) | Uvavanyo oluyi-24/ikhithi Uvavanyo/ikhithi ezingama-48 |
| I-HWTS-TM009 | Ikhithi yokuFumana uTshintsho lweZizalo ze-Human ROS1 (i-Fluorescence PCR) | Uvavanyo oluyi-20/ikhithi Uvavanyo oluyi-50/ikhithi |
| I-HWTS-TM012 | Ikhithi yokuFumanisa utshintsho lwe-EGFR Gene 29 yomntu (i-Fluorescence PCR)） | Uvavanyo/ikhithi ezili-16 Uvavanyo/ikhithi ezingama-32 |
| I-HWTS-TM014 | Ikhithi yokuFumanisa utshintsho lwe-KRAS 8 (i-Fluorescence PCR) | Uvavanyo oluyi-24/ikhithi Uvavanyo/ikhithi ezingama-48 |
| I-HWTS-TM016 | Ikhithi yokuFumana uTshintsho lweMfuzo ye-TEL-AML1 yabantu (i-Fluorescence PCR) | Uvavanyo oluyi-24/ikhithi |
| I-HWTS-GE010 | Ikhithi yokuFumana uTshintsho lweMfuzo ye-BCR-ABL yeHuman (i-Fluorescence PCR) | Uvavanyo oluyi-24/ikhithi |
Ixesha leposi: Epreli-17-2024