Human BRAF Gene V600E Ukuguqulwa

Inkcazelo emfutshane:

Le khithi yovavanyo isetyenziselwa ukukhangela ngokomgangatho ukuguqulwa kofuzo lwe-BRAF ye-V600E kwiisampulu zeparafini ezifakwe kwi-melanoma yomntu, umhlaza we-colorectal, umhlaza wedlala kunye nomhlaza wemiphunga kwi-vitro.


Iinkcukacha zeMveliso

Iithegi zeMveliso

Igama lemveliso

I-HWTS-TM007-Human BRAF Gene V600E Ikhithi yokuFundisa iNguqu(Fluorescence PCR)

Isatifikethi

CE/TFDA

I-Epidemiology

Zingaphezulu kwama-30 iintlobo zotshintsho lwe-BRAF ezifunyenweyo, malunga ne-90% yazo ibekwe kwi-exon 15, apho ukuguqulwa kwe-V600E kubonwa njengelona tshintsho luqhelekileyo, oko kukuthi, i-thymine (T) kwindawo engu-1799 kwi-exon 15 iguqulelwe ku i-adenine (A), okubangelwa ukutshintshwa kwe-valine (V) kwindawo ye-600 nge-glutamic acid (E) kwimveliso yeprotheni.Iinguqu ze-BRAF zihlala zifumaneka kwiithumba ezinobungozi ezifana ne-melanoma, umhlaza we-colorectal, umhlaza we-thyroid kunye nomhlaza wemiphunga.Ukuqonda uguqulo lwejini ye-BRAF ibe yimfuneko yokuhlola i-EGFR-TKIs kunye ne-BRAF gene mutation-targeted drug kunyango lwechiza olujoliswe eklinikhi kwizigulana ezinokuthi zixhamle.

Umjelo

FAM Ukuguqulwa kweV600E, ulawulo lwangaphakathi

IiParameters zobuGcisa

Ugcino

≤-18℃

Beka ubomi kwishelufa

Iinyanga ezili-9

Uhlobo loMfanekiso

iparafini-embedded iisampulu ze-pathological tissue

CV

<5.0%

Ct

≤38

LoD

Sebenzisa iikhithi ukukhangela ulawulo lomgangatho we-LoD oluhambelanayo.a) phantsi kwe-3ng/μL imvelaphi yohlobo lwasendle, i-1% yesantya soguqulo inokubonwa kwi-reaction buffer ngokuzinzileyo;b) ngaphantsi kwe-1% yezinga lokuguqulwa, ukuguqulwa kwe-1 × 103Iikopi / mL kwimvelaphi yohlobo lwasendle lwe-1 × 105Iikopi/mL zinokubonwa ngokuzinzileyo kwi-reaction buffer;c) I-IC Reaction Buffer inokubona owona mda uphantsi wolawulo lomgangatho we-SW3 wolawulo lwangaphakathi lwenkampani.

Izixhobo Ezisebenzayo:

Iinkqubo ze-Biosystems ezisetyenzisiweyo 7500 Iinkqubo zePCR zeXesha langempelaI-Biosystems esetyenzisiweyo ye-7300 ye-Real-Time PCR

Iinkqubo, i-QuantStudio® 5 Iinkqubo ze-PCR zeXesha langempela

Inkqubo yePCR yeLightCycler®480 yeXesha langempela

I-BioRad CFX96 Inkqubo yePCR yexesha lokwenyani

Ukuhamba komsebenzi

Ii-reagents zokutsalwa ezicetyiswayo: QIAGEN's QIAamp DNA FFPE Tissue Kit (56404), iParaffin-embedded Tissue DNA Rapid Extraction Kit (DP330) eyenziwe yiTiangen Biotech(Beijing) Co., Ltd.


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