Orientia tsutsugamushi Nucleic Acid
Igama lemveliso
HWTS-OT002-Orientia tsutsugamushi Nucleic Acid Detection Kit(Fluorescence PCR)
I-Epidemiology
I-Scrub typhus sisifo se-acute febrile esibangelwa usulelo lwe-Orientia tsutsugamushi (Ot). I-Orientia scrub typhus yi-Gram-negative obligate intracellular parasitic microorganism. I-Orientia scrub typhus yeyohlobo lwe-Orientia ngokolandelelwano i-Rickettsiales, usapho i-Rickettsiaceae, kunye nohlobo lwe-Orientia. I-Scrub typhus isasazeka ngokulunywa yimibungu ye-chigger ethwele iintsholongwane. Ibonakala ngesiquphe umkhuhlane ophezulu, i-eschar, i-lymphadenopathy, i-hepatosplenomegaly, kunye ne-peripheral blood leukopenia, njl. Kwiimeko ezinzima, inokubangela ukungasebenzi kwe-meningitis, isibindi kunye nokusilela kwezintso, ukusilela kwe-systemic multi-organ, kunye nokufa.
Iiparamitha zobuGcisa
| Ugcino | ≤-18℃ |
| Beka ubomi kwishelufa | Iinyanga ezili-12 |
| Uhlobo loMfanekiso | iisampuli zeserum |
| CV | ≤5.0% |
| LoD | Iikopi ezingama-500/μL |
| Izixhobo Ezisebenzayo | Isebenza ukuchwetheza i-reagent yokubona: Iinkqubo ze-Biosystems ezisetyenzisiweyo 7500 Iinkqubo zePCR zeXesha langempela, QuantStudio®Iinkqubo zePCR ezi-5 zeXesha langempela, SLAN-96P Iinkqubo zePCR zeXesha lokwenyani (Hongshi Medical Technology Co., Ltd.), I-LineGene 9600 Plus Iinkqubo zokuFumana ze-PCR ngexesha langempela (i-FQD-96A, iteknoloji ye-Hangzhou Bioer), I-MA-6000 yeXesha lokwenyani iBhayisekile yoMyinge weThermal (iSuzhou Molarray Co., Ltd.), Inkqubo yePCR yeBioRad CFX96 yeXesha lokwenyani, BioRad CFX Opus 96 Real-Time PCR System. Isebenza kuhlobo lwe-II yokufumanisa i-reagent: EudemonTMI-AIO800 (HWTS-EQ007) nguJiangsu Macro kunye neMicro-Test Med-Tech Co., Ltd. |
Ukuhamba komsebenzi
IMacro & Micro-Test General DNA/RNA Kit (HWTS-3019) (enokuthi isetyenziswe kunye neMacro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ011)) yiJiangsu Macro & Micro-Test Med-Tech Co., Ltd. Ukukhutshwa kufuneka kuqhutywe ngokuhambelana nomyalelo wokusetyenziswa kwe-reagent. Umthamo wesampulu ekhutshiweyo yi-200μL, kwaye umthamo we-lution ocetyiswayo yi-100μL.
