Iindidi ezingama-28 zoMngcipheko ophezulu weNtsholongwane yePapilloma yoMntu (16/18 Ukuchwetheza) i-Nucleic Acid
Igama lemveliso
I-HWTS-CC006A-28 Iintlobo zeNtsholongwane ePhakamileyo yePapilloma yoMntu (16/18 Ukuchwetheza) Ikhithi yokubona i-Nucleic Acid (Fluorescence PCR)
I-Epidemiology
Umhlaza womlomo wesibeleko lelinye lawona mathumba ayingozi axhaphakileyo kwindlela yokuzala yabasetyhini.Uphononongo lubonise ukuba usulelo oluzingisileyo lwe-HPV kunye nosulelo oluninzi ngomnye woonobangela abaphambili bomhlaza womlomo wesibeleko.Okwangoku, unyango olusebenzayo oluvunyiweyo lusanqongophala lomhlaza womlomo wesibeleko obangelwa yi-HPV, ngoko ke ukufunyanwa kwangoko kunye nothintelo losulelo lomlomo wesibeleko olubangelwa yi-HPV ngundoqo ekuthinteleni umhlaza womlomo wesibeleko.Kubaluleke kakhulu ukuseka uvavanyo olulula, olucacileyo kunye nolukhawulezayo lwe-etiology yokuxilongwa kwekliniki kunye nonyango lomhlaza womlomo wesibeleko.
Umjelo
| Umxube wokusabela | Umjelo | Uhlobo |
| PCR-Mix1 | FAM | 18 |
| VIC(HEX) | 16 | |
| ROX | 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, 68 | |
| CY5 | Ulawulo lwangaphakathi | |
| I-PCR-Mix2 | FAM | 6, 11, 54, 83 |
| VIC(HEX) | 26, 44, 61, 81 | |
| ROX | 40, 42, 43, 53, 73, 82 | |
| CY5 | Ulawulo lwangaphakathi |
IiParameters zobuGcisa
| Ugcino | Ulwelo: ≤-18℃ |
| Beka ubomi kwishelufa | Iinyanga ezili-12 |
| Uhlobo loMfanekiso | I-Swab yeSibeleko, i-Vaginal Swab, Umchamo |
| Ct | ≤28 |
| CV | <5.0% |
| LoD | 300Iikopi/mL |
| Izixhobo Ezisebenzayo | Iinkqubo ze-Biosystems ezisetyenzisiweyo 7500 Iinkqubo zePCR zeXesha langempela Iinkqubo ze-Biosystems eziSetyenzisiweyo 7500 Iinkqubo zePCR ezikhawulezayo QuantStudio®IiNkqubo ze-PCR ze-5 zeXesha langempela SLAN-96P Iinkqubo zePCR zeXesha langempela I-LightCycler®Inkqubo ye-PCR ye-480 yexesha langempela I-LineGene 9600 Plus Real-Time PCR Detection System MA-6000 Real-Time Quantitative Thermal Cycler I-BioRad CFX96 Inkqubo yePCR yexesha lokwenyani BioRad CFX Opus 96 Real-Time PCR System |
Ukuhamba komsebenzi
I-reagent yokutsalwa ecetyiswayo: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (enokuthi isetyenziswe kunye neMacro & Micro-Test I-Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) yi-Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Yongeza i-200μL ye-saline eqhelekileyo ukumisa kwakhona i-pellet kwinqanaba le-2.1, kwaye ke ukutsalwa kufuneka kuqhutywe ngokuhambelana kwimiyalelo yokusetyenziswa kwesi sixhobo sotsalo.Umthamo we-elution ocetyiswayo yi-80μL.
I-reagent yokutsalwa ecetyiswayo: QIAamp DNA Mini Kit (51304) okanye iMacro & Micro-Test Viral DNA/RNA Column (HWTS-3020-50).Yongeza i-200μL ye-saline eqhelekileyo ukumisa kwakhona i-pellet kwinqanaba le-2.1, kwaye emva koko ukukhutshwa kufuneka kuqhutywe ngokwemiyalelo yokusetyenziswa kwesi sixhobo sokukhupha.Umthamo wesampulu okhutshiweyo weisampulu zonke ziyi-200μL, kwaye umthamo we-elution ocetyiswayo yi-100μL.
