Uhlobo lwePoliovirus Ⅱ
Igama lemveliso
I-HWTS-EV007- Uhlobo lwePoliovirus Ⅱ Ikhithi yokuFumanisa i-Nucleic Acid (i-Fluorescence PCR)
I-Epidemiology
I-Poliovirus yintsholongwane ebangela i-poliomyelitis, isifo esosulelayo esisasazeka kakhulu. Le ntsholongwane idla ngokuhlasela inkqubo yemithambo-luvo ephakathi, yonakalisa iiseli zemithambo-luvo ezikwiphondo elingaphambili lomqolo, kwaye ibangele ukukhubazeka kwamalungu omzimba, okuxhaphake kakhulu ebantwaneni, ngoko ke ikwabizwa ngokuba yi-polio. Ii-poliovirus zezohlobo lwe-enterovirus losapho lwe-picornaviridae.
Itshaneli
| I-FAM | Uhlobo lwePoliovirus Ⅱ |
| I-ROX | Ulawulo lwangaphakathi |
Iiparameters zobugcisa
| Indawo yokugcina izinto | ≤-18℃ |
| Beka ubomi kwishelufa | Iinyanga ezi-9 |
| Uhlobo lweSibonelelo | Isampulu yendle esandula ukuqokelelwa |
| Ct | ≤38 |
| CV | ≤5.0% |
| I-LoD | Iikopi ezili-1000/mL |
| Izixhobo Ezisebenzayo | Inkqubo ye-PCR ye-Applied Biosystems 7500 Real-Time, Iinkqubo ze-PCR eziSebenzisiweyo ze-7500 ezikhawulezayo zeXesha langempela I-QuantStudio®Iinkqubo ezi-5 ze-PCR zeXesha langempela Iinkqubo ze-PCR ze-SLAN-96P zeXesha langempela I-LightCycler®Inkqubo ye-PCR yeXesha langempela ye-480 Inkqubo yokuHlola i-PCR yeLineGene 9600 Plus ngexesha langempela I-MA-6000 Real-Time Quantitative Thermal Cycler BInkqubo ye-PCR ye-ioRad CFX96 Real-Time System BioRad CFX Opus 96 Real-Time PCR System |
Ukuhamba komsebenzi
Ukhetho 1.
Ii-reagents zokukhupha ezicetyiswayo: I-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (engasetyenziswa kunye ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B) yiJiangsu Macro & Micro-Test Med-Tech Co., Ltd., Ukukhupha kufuneka kwenziwe ngokwe-IFU ngokungqongqo. Umthamo wokucoca ocetyiswayo yi-80μL.
Ukhetho 2.
Ii-reagents zokukhupha ezicetyiswayo: I-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3022) yiJiangsu Macro & Micro-Test Med-Tech Co., Ltd., Ukukhupha kufuneka kwenziwe ngokwe-IFU ngokungqongqo. Umthamo wokucoca ocetyiswayo yi-100μL.
