Uhlobo lwePoliovirus Ⅱ
Igama lemveliso
HWTS-EV007- Uhlobo lwePoliovirus Ⅱ iNucleic Acid Detection Kit(Fluorescence PCR)
I-Epidemiology
I-Poliovirus yintsholongwane ebangela ipoliomyelitis, isifo esosulelayo esisasazeka ngokubanzi.Le ntsholongwane isoloko ihlasela inkqubo yemithambo-luvo, yonakalise iiseli zemithambo-luvo kuphondo lwangaphambili lomqolo, kwaye ibangele ukukhubazeka kwamalungu, okuxhaphake kakhulu ebantwaneni, ngoko ikwabizwa ngokuba yipoliyo.Iintsholongwane zepoliyo zezohlobo lwe-enterovirus lwentsapho ye-picornaviridae.
Umjelo
FAM | Uhlobo lwePoliovirus Ⅱ |
ROX | Ulawulo lwangaphakathi |
IiParameters zobuGcisa
Ugcino | ≤-18℃ |
Beka ubomi kwishelufa | Iinyanga ezili-9 |
Uhlobo loMfanekiso | Isampulu yesitulo esandula kuqokelelwa |
Ct | ≤38 |
CV | ≤5.0% |
LoD | 1000Iikopi/mL |
Izixhobo Ezisebenzayo | I-Biosystems eSetyenzisiweyo 7500 Inkqubo yePCR yexesha elililo, Iinkqubo ze-Biosystems eziSetyenzisiweyo 7500 Iinkqubo zePCR ezikhawulezayo QuantStudio®IiNkqubo ze-PCR ze-5 zeXesha langempela SLAN-96P Iinkqubo zePCR zeXesha langempela I-LightCycler®Inkqubo ye-PCR ye-480 yexesha langempela I-LineGene 9600 Plus Real-Time PCR Detection System MA-6000 Real-Time Quantitative Thermal Cycler BioRad CFX96 yeXesha lokwenyani PCR System BioRad CFX Opus 96 Ixesha lokwenyani PCR System |
Ukuhamba komsebenzi
Ukhetho loku-1.
Ii-reagents zokutsalwa ezicetyiswayo: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (enokuthi isetyenziswe kunye neMacro & Micro-Test I-Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B) yi-Jiangsu Macro & Micro-Test Med-Tech Co., Ltd., Ukukhutshwa kufuneka kuqhutywe ngokwe-IFU ngokungqongqo Umthamo we-elution ocetyiswayo yi-80μL.
Ukhetho lwesi-2.
I-reagents yokukhutshwa okucetyiswayo: I-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3022) nguJiangsu Macro & Micro-Test Med-Tech Co., Ltd., Ukukhutshwa kufuneka kuqhutywe ngokwe-IFU ngokungqongqo.Umthamo we-elution ocetyiswayo yi-100μL.