IiPathogens zokuphefumla zidibeneyo

IiPathogens zokuphefumla ezidityanisiweyo Umfanekiso okhoyo

Inkcazelo emfutshane:

Le khithi isetyenziselwa ukukhangela umgangatho we-pathogens yokuphefumla kwi-nucleic acid ekhutshwe kwiisampuli ze-oropharyngeal swab zabantu.Iintsholongwane ezichongiweyo ziquka: intsholongwane yomkhuhlane A (H1N1, H3N2, H5N1, H7N9), intsholongwane yomkhuhlane B (Yamataga, Victoria), parainfluenza virus (PIV1, PIV2, PIV3), metapneumovirus (A, B), adenovirus (1, 2, 3) , 4, 5, 7, 55), i-syncytial yokuphefumula (A, B) kunye nentsholongwane yemasisi.

Thumela i-imeyile kuthi

Iinkcukacha zeMveliso

Iithegi zeMveliso

Igama lemveliso

I-HWTS-RT106A-Izixhobo Zokuphefumla eziDityanisiweyo zokuFumana (i-PCR yeFluorescence)

I-Epidemiology

Izifo ezibangelwa yi-pathogenic microorganisms ezihlasela i-pathogenic cavity, umqala, uqhoqhoqho, i-bronchus, imiphunga kunye nezinye izihlunu zokuphefumla kunye namalungu kunye nokuphindaphinda kuthiwa zizifo zendlela yokuphefumula.Iimpawu zeklinikhi ziquka umkhuhlane, ukukhohlela, impumlo ephumayo, umqala obuhlungu, ukudinwa ngokubanzi kunye nokuba buhlungu .Iintsholongwane zokuphefumla ziquka iintsholongwane, i-mycoplasma, i-chlamydia, ibhaktheriya, njl njl. Uninzi lwezifo ezibangelwa yintsholongwane.Iintsholongwane zokuphefumla zineempawu ezilandelayo ezifana neentlobo ezininzi zeentlobo, ukuguquka ngokukhawuleza, ii-subtypes ezinzima, iimpawu ezifanayo zekliniki.Ineempawu zeklinikhi ezifana nokuqala ngokukhawuleza, ukusasazeka ngokukhawuleza, ukosuleleka okunamandla, kunye neempawu ezifanayo ekunzima ukuzichaza, ezibeka isoyikiso esibi kwimpilo yabantu.

Umjelo

FAM	IFV A, IFV B Victoria, PIV uhlobo 1, hMPV uhlobo 2, ADV, RSV uhlobo A, MV·
VIC(HEX)	IFV B, H1, IFV B Yamagata, Isalathiso sangaphakathi
CY5	Isalathiso sangaphakathi, uhlobo lwe-PIV 3, uhlobo lwe-hMPV1, uhlobo lwe-RSV B
ROX	Isalathiso sangaphakathi, i-H3, i-PIV uhlobo lwe-2

IiParameters zobuGcisa

Ugcino	Ulwelo: ≤-18℃ ebumnyameni
Beka ubomi kwishelufa	Iinyanga ezili-9
Uhlobo loMfanekiso	Iiswabs ze-oropharyngeal ezisanda kuqokelela
Ct	≤38
CV	≤5.0%
LoD	500Iikopi/mL
Ukuchaza ngokuthe ngqo	Akukho nto i-cross-reactivity kunye ne-genome yomntu kunye nezinye ii-pathogens zokuphefumla.
Izixhobo Ezisebenzayo	Iyakwazi ukuhambelana nezixhobo zePCR zefluorescent kwimarike. Iinkqubo ze-Biosystems eziSetyenzisiweyo 7500 Iinkqubo zePCR ezikhawulezayo I-QuantStudio®5 Iinkqubo zePCR zeXesha langempela SLAN-96P Iinkqubo zePCR zeXesha langempela Inkqubo yePCR yeLightCycler®480 yeXesha langempela I-LineGene 9600 Plus Real-Time PCR Detection System MA-6000 Real-Time Quantitative Thermal Cycler I-BioRad CFX96 Inkqubo yePCR yexesha lokwenyani BioRad CFX Opus 96 Real-Time PCR System