Iintsholongwane zokuphefumla ezidibeneyo
Igama lemveliso
Ikhithi yokuFumanisa iiPathogens zokuPhefumla ze-HWTS-RT183 (i-Fluorescence PCR)
I-Epidemiology
Isifo seCorona Virus sika-2019, esibizwa ngokuba yi-'COVID-19', sibhekisa kwi-pneumonia ebangelwa lusulelo lwe-SARS-CoV-2. I-SARS-CoV-2 sisifo se-coronavirus esikwi-β genus. I-COVID-19 sisifo esosulelayo esihlaselayo, kwaye uluntu ngokubanzi lusengozini. Okwangoku, umthombo wosulelo ikakhulu zizigulana ezosulelwe yi-2019-nCoV, kwaye abantu abane-symptomatic nabo banokuba ngumthombo wosulelo. Ngokusekelwe kuphando lwangoku lwe-epidemiological, ixesha lokukhulelwa liyi-1-14 yeentsuku, uninzi lweentsuku ezi-3-7. Umkhuhlane, ukukhwehlela okomileyo kunye nokudinwa zezona mpawu ziphambili. Izigulana ezimbalwa zazineempawu ezifana nokuxinana kweempumlo, impumlo egobhozayo, umphimbo obuhlungu, i-myalgia kunye norhudo, njl. Umkhuhlane, owaziwa ngokuba yi-'flu', sisifo esosulelayo esihlaselayo sokuphefumla esibangelwa yintsholongwane yomkhuhlane. Sosulela kakhulu. Sidluliselwa kakhulu ngokukhohlela nokuthimla. Sidla ngokuqhambuka entwasahlobo nasebusika. Iintsholongwane zomkhuhlane zahlulwe zibe yi-influenza A (IFV A), i-influenza B (IFV B), kunye ne-Influenza C (IFV C) iintlobo ezintathu, zonke zezentsholongwane ezinamathelayo, zibangela isifo ebantwini ikakhulu kwiintsholongwane zomkhuhlane A kunye ne-B, yintsholongwane ye-RNA enemigca enye, ehlukaniswe ngezigaba. Intsholongwane ye-Influenza A lusulelo oluhlasela ukuphefumla, oluquka i-H1N1, i-H3N2 kunye nezinye iintlobo, ezichaphazeleka kukuguquka nokuqhuma kwihlabathi liphela. 'Utshintsho' lubhekisa kuguquko lwentsholongwane ye-influenza A, okubangela ukuvela 'kwe-subtype' entsha yentsholongwane. Iintsholongwane ze-Influenza B zahlulwe zibe ziindidi ezimbini, i-Yamagata kunye ne-Victoria Intsholongwane ye-Influenza B ine-antigenic drift kuphela, kwaye ithintela ukujongwa nokupheliswa kwenkqubo yomzimba yokuzikhusela ngokuguqulwa kwayo. Nangona kunjalo, isantya sokuvela kwentsholongwane ye-influenza B sihamba kancinci kunesentsholongwane ye-influenza A yabantu. Intsholongwane ye-Influenza B inokubangela usulelo lokuphefumla kwabantu kwaye ikhokelele kwiindyikityha.
Intsholongwane ye-Respiratory syncytial virus (RSV) yintsholongwane ye-RNA, ephuma kusapho lwe-paramyxoviridae. Idluliselwa ngamathontsi omoya kunye nokudibana ngokusondeleyo kwaye yeyona ntsholongwane iphambili yosulelo lwendlela yokuphefumla esezantsi kwiintsana. Iintsana ezosulelwe yi-RSV zinokuba ne-bronchiolitis enzima kunye ne-pneumonia, ezinxulumene ne-asthma ebantwaneni. Iintsana zineempawu ezinzima, kubandakanya umkhuhlane ophezulu, i-rhinitis, i-pharyngitis kunye ne-laryngitis, kwaye emva koko i-bronchiolitis kunye ne-pneumonia. Abantwana abambalwa abagulayo banokuba nzima nge-otitis media, i-pleurisy kunye ne-myocarditis, njl. Usulelo lwendlela yokuphefumla ephezulu luphawu oluphambili losulelo kubantu abadala nakubantwana abadala.
Iiparameters zobugcisa
| Indawo yokugcina izinto | -18℃ Ebumnyameni |
| Beka ubomi kwishelufa | Iinyanga ezi-9 |
| Uhlobo lweSibonelelo | I-Oropharyngeal swab; I-Nasopharyngeal swab |
| Ct | I-IFV A, i-IFVB, i-RSV, i-SARS-CoV-2, IFV A H1N1Ct≤38 |
| CV | ≤5% |
| I-LoD | Iikopi ezingama-200/mL |
| Ukuchaneka | Iziphumo zokusabela okudibeneyo zibonisa ukuba akukho mpendulo idibeneyo phakathi kwekhithi kunye ne-cytomegalovirus, uhlobo lwe-herpes simplex virus 1, i-varicella zoster virus, i-Epstein-Barr virus, i-adenovirus, i-human metapneumovirus, i-rhinovirus, i-parainfluenza virus type I/II/III/IV, i-bocavirus, i-enterovirus, i-coronavirus, i-Mycoplasma pneumoniae, i-Chlamydia pneumoniae, i-Bordetella pertussis, i-Corynebacterium spp., i-Escherichia coli, i-Haemophilus influenzae, i-Lactobacillus spp., i-Legionella pneumophila, i-Moraxella catarrhalis, iintlobo ezincitshisiweyo ze-Mycobacterium tuberculosis, i-Neisseria meningitidis, i-Neisseria spp., i-Pseudomonas aeruginosa, i-Staphylococcus aureus, i-Streptococcus pneumoniae, i-Staphylococcus epidermidis, i-Streptococcus pyogenes, i-Streptococcus salivarius, i-Acinetobacter baumannii, i-Stenotrophomonas i-maltophilia, i-Burkholderia cepacia, i-Corynebacterium fasciatum, i-Nocardia, i-Serratia marcescens, i-Citrobacter rodentium, i-Cryptococcus, i-Aspergillus fumigatus, i-Aspergillus flavus, i-Pneumocystis carinii, i-Candida albicans, i-Roseburia mucosa, i-Streptococcus oralis, i-Klebsiella pneumoniae, i-Chlamydia psittaci, i-Rickettsia Q fever kunye ne-human genomic nucleic acid. |
| Izixhobo Ezisebenzayo | Iinkqubo ze-PCR eziSebenzisiweyo 7500 zeXesha langempela, iiNkqubo zePCR eziSebenzisiweyo 7500 zeXesha langempela, iQuantStudio®Iinkqubo ezi-5 ze-PCR zeXesha langempela, iiNkqubo ze-SLAN-96P zeXesha langempela ze-PCR (iHongshi Medical Technology Co., Ltd.), iLightCycler®Inkqubo ye-PCR yeXesha Eliqhelekileyo ye-480, iiNkqubo zokuFumanisa i-LineGene 9600 Plus yeXesha Eliqhelekileyo ye-PCR (i-FQD-96A, iteknoloji ye-Hangzhou Bioer), i-MA-6000 yeXesha Eliqhelekileyo ye-Quantitative Thermal Cycler (i-Suzhou Molarray Co., Ltd.), i-BioRad CFX96 yeXesha Eliqhelekileyo ye-PCR, i-BioRad CFX Opus 96 yeXesha Eliqhelekileyo ye-PCR. |
Ukuhamba komsebenzi
I-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (engasetyenziswa kunye ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)), kunye ne-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-8) (engasetyenziswa kunye ne-EudemonTM I-AIO800 (HWTS-EQ007)) yiJiangsu Macro kunye neMicro-Test Med-Tech Co., Ltd.
Umthamo wesampulu ekhutshiweyo yi-200μL kwaye umthamo we-elution ocetyiswayo yi-150μL.
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