IiPathogens zokuphefumla zidibeneyo

IiPathogens zokuphefumla ezidityanisiweyo Umfanekiso okhoyo

Inkcazelo emfutshane:

Le khithi isetyenziselwa ukufunyaniswa komgangatho we-SARS-CoV-2, intsholongwane yomkhuhlane A, intsholongwane yomkhuhlane B, intsholongwane yomkhuhlane A i-H1N1 kunye nokuphefumla kwe-sycytial virus nucleic acids kwi-oropharyngeal swab kunye neesampulu ze-nasopharyngeal swab.

Thumela i-imeyile kuthi

Iinkcukacha zeMveliso

Iithegi zeMveliso

Igama lemveliso

I-HWTS-RT183-Izixhobo Zokuphefumla eziDityanisiweyo zokuFumana (i-Fluorescence PCR)

I-Epidemiology

Isifo se-Corona Virus 2019, esibizwa ngokuba 'yi-COVID-19', sibhekisa kwinyumoniya ebangelwa lusulelo lwe-SARS-CoV-2. I-SARS-CoV-2 yi-coronavirus yodidi lwe-β. I-COVID-19 sisifo esosulelayo esinamandla sokuphefumla, kwaye uluntu lusesichengeni ngokubanzi. Okwangoku, umthombo wosulelo ikakhulu zizigulana ezosulelwe ngu-2019-nCoV, kwaye abantu abosulelekileyo abangenazimpawu nabo banokuba ngumthombo wosulelo. Ngokusekelwe kuphando lwangoku lwe-epidemiological, ixesha lokufukamela liyi-1-14 iintsuku, uninzi lweentsuku ezi-3-7. Umkhuhlane, ukukhwehlela okomileyo kunye nokukhathala zizinto eziphambili zokubonakaliswa. Izigulana ezimbalwa zazineempawu ezifana nokuxinana kweempumlo, impumlo evuzayo, umqala obuhlungu, i-myalgia kunye norhudo, njl. njl. Umkhuhlane, owaziwa ngokuba 'ngumkhuhlane', sisifo esosulelayo esinamandla sokuphefumla esibangelwa yintsholongwane yomkhuhlane. Iyosulela kakhulu. Isasazwa ikakhulu ngokukhohlela kunye nokuthimla. Idla ngokuqhambuka entwasahlobo nasebusika. Iintsholongwane zoMkhuhlane zahlulahlulwe zaba ngumkhuhlane A (IFV A), umkhuhlane B (IFV B), kunye noMkhuhlane C (IFV C) iintlobo ezintathu, zonke zezentsholongwane encangathi, zibangela isifo somntu ikakhulu kwiintsholongwane zomkhuhlane A kunye no-B, yintsholongwane enye, i-RNA ecandekileyo. Intsholongwane ye-Influenza A lusulelo olubukhali lokuphefumla, kubandakanywa i-H1N1, i-H3N2 kunye nezinye ii-subtypes, ezivame ukuguquka kunye nokuqhambuka kwihlabathi jikelele. 'I-Shift' ibhekiselele kutshintsho lwentsholongwane yomkhuhlane A, ekhokelela ekuveleni kwentsholongwane entsha 'uhlobo oluncinane'. Iintsholongwane zoMkhuhlane B zahlulwe zibe yimizila emibini, iYamagata kunye neVictoria B virus ine-antigenic drift kuphela, kwaye iyaluphepha uphononongo lwenkqubo yokugonyela komntu kunye nokuphelisa ngokuguqulwa kwayo. Nangona kunjalo, isantya sokuvela kwentsholongwane yomkhuhlane we-B siyacotha kuneso sentsholongwane yomkhuhlane womntu. Intsholongwane ye-Influenza B isenokubangela usulelo lokuphefumla ebantwini kwaye ikhokelele kwindyikityha yezifo.

Intsholongwane ye-Respiratory syncytial virus (RSV) yintsholongwane ye-RNA, yosapho lwe-paramyxoviridae. Isasazwa ngamaconsi omoya kunye noqhagamshelwano olusondeleyo kwaye iyona pathogen ephambili yokusuleleka kwintsholongwane yokuphefumula kwiintsana. Iintsana ezosulelwe yi-RSV zinokuba ne-bronchiolitis ebukhali kunye nenyumoniya, ehambelana ne-asthma ebantwaneni. Iintsana zineempawu ezinzima, ezibandakanya umkhuhlane ophezulu, i-rhinitis, i-pharyngitis kunye ne-laryngitis, kwaye emva koko i-bronchiolitis kunye ne-pneumonia. Abantwana abambalwa abagulayo banokuba nzima kunye ne-otitis media, i-pleurisy kunye ne-myocarditis, njl njl.Usulelo lwendlela yokuphefumula ephezulu luphawu oluphambili lokusuleleka kubantu abadala kunye nabantwana abadala.

Iiparamitha zobuGcisa

Ugcino	-18℃ ebumnyameni
Beka ubomi kwishelufa	Iinyanga ezili-9
Uhlobo loMfanekiso	i-oropharyngeal swab; I-Nasopharyngeal swab
Ct	IFV A, IFVB, RSV, SARS-CoV-2, IFV A H1N1Ct≤38
CV	≤5%
LoD	Iikopi ezingama-200/μL
Ukuchaza ngokuthe ngqo	Iziphumo ze-cross-reactivity zibonisa ukuba akukho mpendulo phakathi kwekiti kunye ne-cytomegalovirus, i-herpes simplex virus type 1, i-varicella zoster virus, i-virus ye-Epstein-Barr, i-adenovirus, i-metapneumovirus yabantu, i-rhinovirus, uhlobo lwentsholongwane ye-parainfluenza I / II / III / IV, bocavirus, enterovirus, coronavirus, Mycoplasmet pneumonia, Chlamydia Corynebacterium spp., Escherichia coli, Haemophilus influenzae, Lactobacillus spp., Legionella pneumophila, Moraxella catarrhalis, attenuated strains of Mycobacterium tuberculosis, Neisseria meningitidis, Neisseria spp., Stauusseria spp. Streptococcus pneumoniae, Staphylococcus epidermidis, Streptococcus pyogenes, Streptococcus salivarius, Acinetobacter baumannii, Stenotrophomonas maltophilia, Burkholderia cepacia, Corynebacterium fasciatum, Nocardia, Serraticternscocus, Cryptococter, Serratictercocus martophia, i-Cryptococter, i-Cryptococter, i-Cryptococter, i-Cryptococter, i-martophia, Aspergillus fumigatus, Aspergillus flavus, Pneumocystis carinii, Candida albicans, Roseburia mucosa, Streptococcus oralis, Klebsiella pneumoniae, Chlamydia psittaci, Rickettsia Q fever kunye human genomic nucleic acid.
Izixhobo Ezisebenzayo	Iinkqubo ze-Biosystems eziSetyenzisiweyo 7500 IiNkqubo ze-PCR zeXesha langempela, ii-Biosystems eziSetyenzisiweyo 7500 Iinkqubo zePCR ezikhawulezayo, i-QuantStudio^®IiNkqubo ze-PCR ze-5 zexesha langempela, i-SLAN-96P ye-Real-Time PCR Systems (Hongshi Medical Technology Co., Ltd.), i-LightCycler^®Inkqubo ye-PCR ye-480 ye-Real-Time, i-LineGene 9600 Plus ye-Real-Time PCR Detection Systems (FQD-96A, iteknoloji ye-Hangzhou Bioer), i-MA-6000 ye-Real-Time Quantitative Thermal Cycler (i-Suzhou Molarray Co., Ltd.), i-BioRad CFX96 ye-Real-Time PCR System, i-BioRad System ye-PCR ye-BioRad.

Ukuhamba komsebenzi

IMacro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (enokuthi isetyenziswe kunye neMacro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)), kunye neMacro & Micro-Test Viral DNA/RNA Kit (HWTS-17u-30)^TMI-AIO800 (HWTS-EQ007)) yi-Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.

Umthamo wesampulu ekhutshiweyo yi-200μL kwaye umthamo we-elution ocetyiswayo yi-150μL.

Ngaphambili: ILegionella Pneumophila Nucleic Acid Detection Kit

Okulandelayo: Umkhenkce-omisiweyo Iindidi ezili-11 zeePathogens zokuphefumla iNucleic Acid

Bhala umyalezo wakho apha kwaye uwuthumele kuthi