Iintlobo ezintandathu zezifo zokuphefumla
Igama lemveliso
Ikhithi ye-HWTS-OT058A/B/C/Z-I-Real time fluorescent RT-PCR yokubona iintlobo ezintandathu zezifo zokuphefumla
Isatifikethi
CE
I-Epidemiology
Isifo seCorona Virus sika-2019, esibizwa ngokuba yi-"COVID-19", sibhekisa kwi-pneumonia ebangelwa lusulelo lwe-SARS-CoV-2. I-SARS-CoV-2 sisifo se-coronavirus esikwi-β genus. I-COVID-19 sisifo esosulelayo esihlaselayo, kwaye uluntu ngokubanzi lusengozini. Okwangoku, umthombo wosulelo ikakhulu zizigulana ezosulelwe yi-SARS-CoV-2, kwaye abantu abane-symptomatic nabo banokuba ngumthombo wosulelo. Ngokusekelwe kuphando lwangoku lwe-epidemiological, ixesha lokukhulelwa liphakathi kweentsuku ezili-1-14, uninzi lwazo ziintsuku ezi-3-7. Umkhuhlane, ukukhwehlela okomileyo kunye nokudinwa zezona mpawu ziphambili. Izigulana ezimbalwa zazine-impumlo exineneyo, impumlo egobhozayo, umphimbo obuhlungu, i-myalgia kunye norhudo.
I-Influenza, eyaziwa ngokuba yi-"flu", sisifo esosulelayo esibangelwa yintsholongwane ye-influenza. Isasazeka kakhulu. Isasazeka kakhulu ngokukhohlela nokuthimla. Idla ngokuqhambuka entwasahlobo nasebusika. Iintsholongwane ze-Influenza zahlulwe zibe yi-influenza A, IFV A, influenza B, IFV B, kunye ne-Influenza C, IFV C iintlobo ezintathu, zonke zezentsholongwane ezinamathelayo, zibangela isifo ebantwini ikakhulu kwiintsholongwane ze-influenza A kunye ne-B, yintsholongwane ye-RNA enemigca enye, ehlukaniswe ngezigaba. Intsholongwane ye-Influenza A sisifo sokuphefumla esikhawulezileyo, kuquka i-H1N1, i-H3N2 kunye nezinye iintlobo, ezinokuthi ziguquke kwaye ziqhambuke kwihlabathi liphela. Igama elithi "Shift" libhekisa kuguquko lwentsholongwane ye-influenza A, nto leyo ebangela ukuvela kwentsholongwane entsha "engaphantsi". Iintsholongwane ze-Influenza B zahlulwe ngemigca emibini, i-Yamagata kunye ne-Victoria. Intsholongwane ye-Influenza B ine-antigenic drift kuphela, kwaye ithintela ukujongwa nokupheliswa kwenkqubo yomzimba yokuzikhusela ngoguquko lwayo. Nangona kunjalo, isantya sokuvela kwentsholongwane ye-influenza B sihamba kancinci kunesentsholongwane ye-influenza A yabantu. Intsholongwane ye-Influenza B inokubangela izifo zokuphefumla ebantwini kwaye ikhokelele kwiindyikityha zobhubhane.
I-Adenovirus (AdV) yeye-adenovirus yezilwanyana ezincancisayo, eyi-DNA virus ephindwe kabini ngaphandle kwe-enclosure. Ubuncinane ii-genotypes ezingama-90 zifunyenwe, ezinokwahlulwa zibe yi-AG 7 subgenera. Ukosuleleka yi-AdV kunokubangela izifo ezahlukeneyo, kubandakanya i-pneumonia, i-bronchitis, i-cystitis, i-eye conjunctivitis, izifo zesisu kunye ne-encephalitis. I-Adenovirus pneumonia yenye yezona ntlobo zimbi ze-pneumonia efunyanwa luluntu ebantwaneni, ibangela malunga ne-4%-10% ye-pneumonia efunyanwa luluntu.
I-Mycoplasma pneumoniae (MP) luhlobo lwe-microorganism encinci ye-prokaryotic, ephakathi kwebhaktheriya kunye nentsholongwane, enesakhiwo seseli kodwa ingenaludonga lweseli. I-MP ikakhulu ibangela usulelo lwendlela yokuphefumla yabantu, ngakumbi kubantwana nakubantu abancinci. Ingabangela i-human mycoplasma pneumonia, usulelo lwendlela yokuphefumla yabantwana kunye ne-atypical pneumonia. Iimpawu zeklinikhi zahlukile, uninzi lwazo kukukhwehlela okukhulu, umkhuhlane, ukubanda, intloko ebuhlungu, umphimbo obuhlungu. Usulelo lwendlela yokuphefumla ephezulu kunye ne-bronchial pneumonia zezona zixhaphakileyo. Ezinye izigulana zinokuvela kusulelo lwendlela yokuphefumla ephezulu ukuya kwi-pneumonia enzima, uxinzelelo olukhulu lokuphefumla kunye nokufa kunokwenzeka.
Intsholongwane ye-Respiratory syncytial virus (RSV) yintsholongwane ye-RNA, ephuma kusapho lwe-paramyxoviridae. Idluliselwa ngamathontsi omoya kunye nokudibana ngokusondeleyo kwaye yeyona ntsholongwane iphambili yosulelo lwendlela yokuphefumla esezantsi kwiintsana. Iintsana ezosulelwe yi-RSV zinokuba ne-bronchiolitis enzima (ebizwa ngokuba yi-bronchiolitis) kunye ne-pneumonia, ezinxulumene ne-asthma ebantwaneni. Iintsana zineempawu ezinzima, kubandakanya umkhuhlane ophezulu, i-rhinitis, i-pharyngitis kunye ne-laryngitis, kwaye emva koko i-bronchiolitis kunye ne-pneumonia. Abantwana abambalwa abagulayo banokuba nzima nge-otitis media, i-pleurisy kunye ne-myocarditis, njl. Usulelo lwendlela yokuphefumla ephezulu luphawu oluphambili losulelo kubantu abadala nakubantwana abadala.
Itshaneli
| Igama lejelo | I-R6 Reaction Buffer A | I-R6 Reaction Buffer B |
| I-FAM | I-SARS-CoV-2 | I-HAdV |
| I-VIC/HEX | Ulawulo lwangaphakathi | Ulawulo lwangaphakathi |
| CY5 | IFV A | MP |
| I-ROX | IFV B | I-RSV |
Iiparameters zobugcisa
| Indawo yokugcina izinto | Ulwelo: ≤-18℃ Ebumnyameni; I-Lyophilized: ≤30℃ Ebumnyameni |
| Beka ubomi kwishelufa | Ulwelo: Iinyanga ezili-9; I-Lyophilized: Iinyanga ezili-12 |
| Uhlobo lweSibonelelo | Igazi elipheleleyo, iPlasma, iSerum |
| Ct | ≤38 |
| CV | ≤5.0％ |
| I-LoD | Iikopi ezingama-300/mL |
| Ukuchaneka | Iziphumo zokudibana kweziphumo zibonise ukuba akukho mpendulo idibeneyo phakathi kwekhithi kunye ne-human coronavirus SARSr-CoV, MERSr-CoV, HCoV-OC43, HCoV-229E, HCoV-HKU1, HCoV-NL63, i-parainfluenza virus type 1, 2, 3, rhinovirus A, B, C, chlamydia pneumoniae, human metapneumovirus, enterovirus A, B, C, D, human pulmonary virus, epstein-barr virus, measles virus, human cytomegalo virus, rotavirus, norovirus, parotitis virus, varicella-zoster virus, legionella, bordetella pertussis, haemophilus influenzae, staphylococcus aureus, streptococcus pneumoniae, s. pyogenes, klebsiella pneumoniae, mycobacterium tuberculosis, smoke aspergillus, candida albicans, candida glabrata, pneumocystis jiroveci kunye ne-cryptococcus esandul’ ukuzalwa kunye ne-human genomic nucleic acid. |
| Izixhobo Ezisebenzayo | Ingalingana nezixhobo ze-PCR ezikhanyayo ezithengiswayo kwimarikeIinkqubo ze-PCR ze-SLAN-96P zeXesha langempela Iinkqubo ze-ABI 7500 ze-Real-Time PCR Iinkqubo ze-PCR ze-ABI 7500 ezikhawulezayo zeXesha langempela I-QuantStudio®Iinkqubo ezi-5 ze-PCR zeXesha langempela I-LightCycler®Iinkqubo ze-PCR zeXesha langempela ezingama-480 Iinkqubo zokuFumanisa i-LineGene 9600 Plus zeXesha langempela ze-PCR I-MA-6000 Real-Time Quantitative Thermal Cycler Inkqubo ye-PCR ye-BioRad CFX96 yeXesha langempela, i-BioRad Inkqubo yePCR yeXesha langempela yeCFX Opus 96 |
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