I-SARS-CoV-2 influenza A influenza B Nucleic Acid edibeneyo
Igama lemveliso
Ikhithi yokuFumanisa i-HWTS-RT060A-SARS-CoV-2 influenza A influenza B Nucleic Acid Combined (Fluorescence PCR)
Isatifikethi
I-AKL/TGA/CE
I-Epidemiology
Isifo seCorona Virus sika-2019 (i-COVID-19) sibangelwa yi-SARS-CoV-2 eyinxalenye ye-β Coronavirus yohlobo. I-COVID-19 sisifo esosulelayo esihlasela ukuphefumla, kwaye uninzi lwabantu lusengozini. Okwangoku, abaguli abane-SARS-CoV-2 ngabona babangela usulelo, kwaye abaguli abangenazo iimpawu nabo banokuba ngumthombo wosulelo. Ngokusekelwe kuphando lwangoku lwe-epidemiological, ixesha lokufunxa liphakathi kweentsuku ezili-1-14, uninzi lwazo iintsuku ezi-3-7. Iimpawu eziphambili yayikumkhuhlane, ukukhwehlela okomileyo kunye nokudinwa. Abaguli abambalwa baneempawu ezifana nokuxinana kweempumlo, impumlo egobhozayo, umphimbo obuhlungu, i-myalgia kunye norhudo.
I-Influenza sisifo sokuphefumla esibangelwa yintsholongwane ye-influenza. Sisasazeka kakhulu kwaye sisasazeka ngokukhohlela nokuthimla. Sidla ngokuqhambuka entwasahlobo nasebusika. Kukho iintlobo ezintathu ze-Influenza, i-Influenza A (IFV A), i-Influenza B (IFV B) kunye ne-Influenza C (IFV C), zombini ziyinxalenye yosapho lwe-ortomyxovirus. I-Influenza A kunye ne-B, eziyi-single-stranded, segmental RNA viruses, zezona zizathu ziphambili zezifo zabantu. I-Influenza A sisifo esosulelayo sokuphefumla esibukhali, kuquka i-H1N1, i-H3N2 kunye nezinye iintlobo, kulula ukutshintsha. Ukuqhambuka kwehlabathi, "utshintsho" lubhekisa kuguquko lwe-influenza A, okubangela "uhlobo oluncinci" olutsha lwe-viral. I-Influenza B yahlulwe yaba ziindidi ezimbini: i-Yamagata kunye ne-Victoria. I-Influenza B ine-antigenic drift kuphela, kwaye ibaleka ukujonga nokuphelisa yinkqubo yomzimba yokuzikhusela ngoguquko. Kodwa iintsholongwane ze-influenza B ziguquka kancinci kune-influenza A yabantu, ekwabangela usulelo lokuphefumla kunye nobhubhane ebantwini.
Itshaneli
| I-FAM | I-SARS-CoV-2 |
| I-ROX | IFV B |
| CY5 | IFV A |
| I-VIC(HEX) | Izakhi zofuzo zoLawulo lwangaphakathi |
Iiparameters zobugcisa
| Indawo yokugcina izinto | Ulwelo: ≤-18℃ Ebumnyameni |
| Ukwenziwa kwe-Lyophilization: ≤30℃ ebumnyameni | |
| Beka ubomi kwishelufa | Ulwelo: Iinyanga ezili-9 |
| I-Lyophilization: iinyanga ezili-12 | |
| Uhlobo lweSibonelelo | Iiswabhu zeNasopharyngeal, iiswabhu zeOropharyngeal |
| Ct | ≤38 |
| CV | ≤5.0% |
| I-LoD | Iikopi ezingama-300/mL |
| Ukuchaneka | Iziphumo zovavanyo oludibeneyo zibonise ukuba le khithi iyahambelana ne-human coronavirus SARSr-CoV, MERSr-CoV, HcoV-OC43, HcoV-229E, HcoV-HKU1, HCoV-NL63, i-respiratory syncytial virus A kunye ne-B, i-parainfluenza virus 1, 2 kunye ne-3, i-rhinovirusA, B kunye ne-C, i-adenovirus 1, 2, 3, 4, 5, 7 kunye ne-55, i-human metapneumovirus, i-enterovirus A, B, C kunye ne-D, i-human cytoplasmic pulmonary virus, i-EB virus, i-measles virus I-Human cytomegalovirus, i-rotavirus, i-norovirus, i-mumps virus, i-varicella zoster virus, i-Mycoplasma pneumoniae, i-Chlamydia pneumoniae, i-Legionella, i-pertussis, i-Haemophilus influenzae, i-Staphylococcus aureus, i-Streptococcus pneumoniae, i-Streptococcus pyogenes, i-Klebsiella pneumoniae, i-Mycobacterium tuberculosis, i-Aspergillus i-fumigatus, i-Candida albicans, i-Candida glabrata Akukho mpendulo idibeneyo phakathi kwe-Pneumocystis yersini kunye ne-Cryptococcus neoformans. |
| Izixhobo Ezisebenzayo: | Ingalingana nezixhobo zePCR ezikhanyayo ezithengiswayo. Iinkqubo ze-PCR ze-Applied Biosystems 7500 Real-Time Iinkqubo ze-PCR eziSebenzisiweyo ze-7500 ezikhawulezayo zeXesha langempela Iinkqubo ze-PCR zeQuantStudio®5 zeXesha langempela Iinkqubo ze-PCR ze-SLAN-96P zeXesha langempela Inkqubo ye-PCR yeLightCycler®480 yeXesha langempela Inkqubo yokuHlola i-PCR yeLineGene 9600 Plus ngexesha langempela I-MA-6000 Real-Time Quantitative Thermal Cycler Inkqubo ye-PCR ye-BioRad CFX96 yeXesha langempela Inkqubo ye-PCR ye-BioRad CFX Opus 96 yeXesha langempela |
Ukuhamba komsebenzi
Ukhetho 1.
I-reagent yokukhupha ecetyiswayo: I-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3001, HWTS-3004-32, HWTS-3004-48) kunye ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006).
Ukhetho 2.
I-reagent yokukhupha ecetyiswayo: I-Nucleic Acid Extraction okanye i-Purification Reagent (YDP302) yiTiangen Biotech (Beijing) Co.,Ltd.
