SARS-CoV-2 umkhuhlane A umkhuhlane B Nucleic Acid Idityanisiwe
Igama lemveliso
HWTS-RT060A-SARS-CoV-2 umkhuhlane A umkhuhlane B iNucleic Acid eDityanisiweyo yeKhithi yokubona (i-Fluorescence PCR)
Isatifikethi
AKL/TGA/CE
I-Epidemiology
I-Corona Virus Disease 2019 (COVID-19) ibangelwa yi-SARS-CoV-2 eyeye-β Coronavirus yodidi. I-COVID-19 sisifo esosulelayo esinamandla sokuphefumla, kwaye isihlwele sichaphazeleka ngokubanzi. Okwangoku, abaguli abosulelwe yi-SARS-CoV-2 ngoyena mthombo wosulelo, kwaye izigulana ezingenazimpawu zinokuba ngumthombo wosulelo. Ngokusekelwe kuphando lwangoku lwe-epidemiological, ixesha lokufukamela liyi-1-14 iintsuku, uninzi lweentsuku ezi-3-7. Eyona nto yayibonakaliswa ngumkhuhlane, ukukhohlela okomileyo kunye nokudinwa. Izigulana ezimbalwa zineempawu ezifana nokuxinana kweempumlo, impumlo ebalekayo, umqala obuhlungu, i-myalgia kunye norhudo.
Umkhuhlane lusulelo olubukhali lokuphefumla olubangelwa yintsholongwane yomkhuhlane. Iyosulela kakhulu kwaye isasazeka kakhulu ngokukhohlela kunye nokuthimla. Idla ngokuqhambuka entwasahlobo nasebusika. Zintathu iindidi zoMkhuhlane, uMkhuhlane A (IFV A), uMkhuhlane B (IFV B) kunye noMkhuhlane C (IFV C), zombini ziphuma kusapho lwe-ortomyxovirus. I-Influenza A kunye ne-B, ene-single-stranded, i-segmental RNA viruses, yizona zizathu eziphambili zezifo zabantu. I-Influenza A sisifo esosulelayo esinamandla sokuphefumula, kuquka i-H1N1, i-H3N2 kunye nezinye ii-subtypes, kulula ukuyitshintsha. Uqhambuko lwehlabathi jikelele, "i-shift" ibhekisa kuguquko lomkhuhlane A, olukhokelela kuhlobo olutsha lwentsholongwane. Umkhuhlane B wohlulwe ngokwemizila emibini: Yamagata kunye neVictoria. Umkhuhlane B une-antigenic drift kuphela, kwaye bayakuphepha ukubekw' esweni kunye nokupheliswa kwenkqubo yomzimba yokuzikhusela ngoguqulo. Kodwa iintsholongwane zomkhuhlane B zivela kancinane kunomkhuhlane womntu A, wona obangela usulelo lokuphefumla kunye nobhubhane ebantwini.
Umjelo
| FAM | I-SARS-CoV-2 |
| ROX | IFV B |
| CY5 | IFV A |
| VIC(HEX) | Ijene yoLawulo lwangaphakathi |
Iiparamitha zobuGcisa
| Ugcino | Ulwelo: ≤-18℃ ebumnyameni |
| I-Lyophilization: ≤30℃ ebumnyameni | |
| Beka ubomi kwishelufa | Ulwelo: iinyanga ezili-9 |
| I-Lyophilization: iinyanga ezili-12 | |
| Uhlobo loMfanekiso | I-Nasopharyngeal swabs, i-Oropharyngeal swabs |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | Iikopi ezingama-300/mL |
| Ukuchaza ngokuthe ngqo | Iziphumo zovavanyo ezinqamlezileyo zibonise ukuba ikhithi iyahambelana ne-coronavirus yabantu i-SARSr- CoV, i-MERSr-CoV, i-HcoV-OC43, i-HcoV-229E, i-HcoV-HKU1, i-HCoV-NL63, intsholongwane yokuphefumla ye-syncytial virus A kunye ne-B, i-parainfluenza virus 1, 2 kunye ne-3, irhinovirus, i-adenovirus, i-adenovirus, i-adenovirus, i-ade, i-3, i-adenovirus, i-ade, i-3, i-virus I-5, i-7 kunye ne-55, i-metapneumovirus yabantu, i-enterovirus A, B, C kunye ne-D, i-virus ye-cytoplasmic pulmonary virus, i-virus ye-EB, i-virus yemasisi I-cytomegalovirus yabantu, i-rotavirus, i-norovirus, i-mumps virus, i-varicella zoster virus, i-Mycoplasma pneumoniae, i-Chlamydia pneumoniaetus, i-Staphylophilophilus e-Legionela, i-Haesisfluenza aureus, Streptococcus pneumoniae, Streptococcus pyogenes, Klebsiella pneumoniae, Mycobacterium tuberculosis, Aspergillus fumigatus, Candida albicans, Candida glabrata Kwakungekho mpendulo phakathi kwePneumocystis yersini kunye neCryptococcus neoformans. |
| Izixhobo Ezisebenzayo: | Iyakwazi ukuhambelana nezixhobo zePCR zefluorescent kwimarike. Iinkqubo ze-Biosystems ezisetyenzisiweyo 7500 Iinkqubo zePCR zeXesha langempela Iinkqubo ze-Biosystems eziSetyenzisiweyo 7500 Iinkqubo zePCR ezikhawulezayo I-QuantStudio®5 Iinkqubo zePCR zeXesha langempela SLAN-96P Iinkqubo zePCR zeXesha langempela Inkqubo yePCR yeLightCycler®480 yeXesha langempela I-LineGene 9600 Plus Real-Time PCR Detection System MA-6000 Real-Time Quantitative Thermal Cycler I-BioRad CFX96 Inkqubo yePCR yexesha lokwenene BioRad CFX Opus 96 Real-Time PCR System |
Ukuhamba komsebenzi
Ukhetho loku-1.
I-reagent yokutsalwa ecetyiswayo: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3001, HWTS-3004-32, HWTS-3004-48) kunye neMacro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006).
Ukhetho lwesi-2.
I-Reagent yokutsalwa ecetyiswayo: I-Nucleic Acid Extraction okanye i-Reagent yokucoca (YDP302) yi-Tiangen Biotech(Beijing) Co.,Ltd.
