Ukwahluka kwe-SARS-CoV-2
Igama lemveliso
I-HWTS-RT072A-SARS-CoV-2 Izixhobo Zokuchongwa kweeNqununu (Fluorescence PCR)
Isatifikethi
CE
I-Epidemiology
Inoveli coronavirus (SARS-CoV-2) isasazeke kwinqanaba elikhulu kwihlabathi liphela.Kwinkqubo yokusasaza, utshintsho olutsha luhlala lusenzeka, lukhokelela kutshintsho olutsha.Le mveliso isetyenziselwa ukufumanisa okuncedisayo kunye nokwahlulwa kwamatyala anxulumene nosulelo emva kokusasazeka okukhulu kwe-Alpha, Beta, Gamma, Delta kunye ne-Omicron yokuguquguquka kweentlobo ukusukela ngoDisemba ka-2020.
Umjelo
| FAM | N501Y, HV69-70del |
| CY5 | 211-212del, K417N |
| VIC(HEX) | E484K, Ulawulo lwangaphakathi |
| ROX | I-P681H, L452R |
IiParameters zobuGcisa
| Ugcino | ≤-18℃ ebumnyameni |
| Beka ubomi kwishelufa | Iinyanga ezili-9 |
| Uhlobo loMfanekiso | i-nasopharyngeal swabs, i-oropharyngeal swabs |
| CV | ≤5.0% |
| Ct | ≤38 |
| LoD | 1000Iikopi/mL |
| Ukuchaza ngokuthe ngqo | Akukho kuphinda-phinda kusebenze kunye ne-coronavirus yabantu i-SARS-CoV kunye nezinye iipathogens eziqhelekileyo. |
| Izixhobo Ezisebenzayo: | IQuantStudio™5 Iinkqubo zePCR zeXesha langempela I-SLAN ®-96P Iinkqubo ze-PCR zeXesha langempela Inkqubo yePCR yeLightCycler®480 yeXesha langempela I-LineGene 9600 Plus Real-Time PCR Detection System MA-6000 Real-Time Quantitative Thermal Cycler I-BioRad CFX96 Inkqubo yePCR yexesha lokwenyani BioRad CFX Opus 96 Real-Time PCR System |
Ukuhamba komsebenzi
Ukhetho loku-1.
I-reagent yokutsalwa ecetyiswayo: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3001, HWTS-3004-32, HWTS-3004-48) kunye neMacro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006).
Ukhetho lwesi-2.
I-Reagent yokutsalwa ecetyiswayo: Ukutsalwa kwe-Nucleic Acid okanye i-Reagent yokucoca (YDP302) yi-Tiangen Biotech(Beijing) Co.,Ltd.
