IiPathogens eziNtanda zokuphefumla
Igama lemveliso
I-HWTS-RT175-Six-Six Pathogens eziphefumlayo kwiNucleic Acid Detection Kit (Fluorescence PCR)
I-Epidemiology
Usulelo lokuphefumla lelona qela lixhaphakileyo lezifo zabantu ezinokuthi zenzeke nakwesiphi na isini, ubudala kunye nommandla wejografi kwaye yenye yezona zizathu zibalulekileyo zokugula nokufa kwabantu kwihlabathi liphela. Iintsholongwane zeklinikhi eziqhelekileyo zokuphefumla ziquka intsholongwane yokuphefumla ye-syncytial virus, i-adenovirus, i-metapneumovirus yabantu, i-rhinovirus, i-parainfluenza virus (I/II/III) kunye ne-Mycoplasma pneumoniae. Iimpawu zeklinikhi kunye neempawu ezibangelwa usulelo lwendlela yokuphefumula ziyafana, kodwa unyango, ukusebenza kakuhle, kunye nobude besifo siyahluka phakathi kwezifo ezibangelwa zizifo ezahlukeneyo. Okwangoku, ezona ndlela ziphambili zokufumanisa ezi ntsholongwane zingasentla zokuphefumla zibandakanya: ukuhlukaniswa kwentsholongwane, ukubhaqwa kwe-antigen kunye nokufumanisa i-nucleic acid. Le khithi inceda ekuxilongeni usulelo lwentsholongwane yokuphefumla ngokufumanisa kunye nokuchonga i-nucleic acids yentsholongwane ethile kubantu abaneempawu kunye neempawu zosulelo lokuphefumla, ngokudibanisa nezinye iziphumo zeklinikhi kunye nezaselebhu.
Iiparamitha zobuGcisa
| Ugcino | ≤-18℃ |
| Beka ubomi kwishelufa | Iinyanga ezili-12 |
| Uhlobo loMfanekiso | Isampuli ye-Oropharyngeal swab |
| Ct | Adv, PIV, MP, RhV, hMPV, RSV Ct≤38 |
| CV | <5.0% |
| LoD | I-LoD ye-Adv, iMP, i-RSV, i-hMPV, i-RhV kunye ne-PIV zonke ziyi-200Copies/mL |
| Ukuchaza ngokuthe ngqo | Iziphumo zovavanyo lwe-cross-reactivity zibonise ukuba akukho-cross-reactivity phakathi kwekiti kunye ne-coronavirus yenoveli, intsholongwane yomkhuhlane A, intsholongwane yomkhuhlane B, i-bocavirus yabantu, i-cytomegalovirus, i-herpes simplex virus type 1, i-varicella zoster virus, i-EBV, i-pertussis bacillus, i-Chlamydophila pneumoniae, i-Corynebacterium, i-Ecorynebacterium, i-Ecorynebacteria, i-Escherichicophilia, i-Ecorynebacterium, i-Escherichichi, i-Escherichiacolum, Lactobacillus spp, Legionella pneumophila, C. catarrhalis, kwaye attenuated strains of Mycobacterium tuberculosis, Neisseria meningitidis, Neisseria spp, Pseudomonas aeruginosa, Staphylococcus aureus, Staphylococcus epidermicoccuseptococcus, St. Streptococcus salivarius, Actinobacillus baumannii, ukondla kancinci maltophilic monococci, Burkholderia maltophilia, Streptococcus striatus, Nocardia sp., Sarcophaga viscosa, Citrobacter citriodora, Cryptococcus spp, Aspertusflaugalus, Aspertus, Aspertus, Aspertus, Aspertus, Aspertus, Aspertus, Aspertus, Aspertus, P, Candida albicans, Rohypnogonia viscera, oral streptococci, Klebsiella pneumoniae, Chlamydia psittaci, Rickettsia q fever kunye human genomic nucleic acids. I-Anti-interfering amandla: i-mucin (60 mg / mL), igazi lomntu, i-benfotiamine (2 mg / mL), i-oxymetazoline (2 mg / mL), i-sodium chloride (20 mg / mL), i-beclomethasone (20 mg / mL), i-dexamethasone (20 mg / mL), i-flunitrazolone / i-lone acetone (20mL) mg/mL), budesonide (1 mg/mL), mometasone (2 mg/mL), fluticasone (2 mg/mL), histamine hydrochloride (5 mg/mL), intranasal live influenza virus vaccine, benzocaine (10%), menthol (10%), zanamivir (20 mg/virin/mL), iparamivir (20 mg/mL/mL) i-oseltamivir (0.15 mg / mL), i-mupirocin (20 mg / mL), i-tobramycin (0.6 mg / mL), i-UTM, i-saline, i-guanidine hydrochloride (5 M / L), i-Tris (2 M / L), i-ENTA-2Na (0.6 M / L), i-trilostane (15%), i-isopropyl, i-alcohol (i-1%) kunye ne-alcohol (i-2%). Uvavanyo lokuphazamiseka, iziphumo zibonise ukuba akukho mpendulo yokuphazamiseka kwiziphumo zokufumanisa i-pathogen kwimigxina engentla yezinto eziphazamisayo. |
| Izixhobo Ezisebenzayo | SLAN-96P Iinkqubo zePCR zeXesha langempela Isetyenziselwe i-Biosystems ye-7500 ye-Real-Time PCR System QuantStudio®IiNkqubo ze-PCR ze-5 zeXesha langempela I-LineGene 9600 Plus Real-Time PCR Detection System MA-6000 Real-Time Quantitative Thermal Cycler I-BioRad CFX96 Inkqubo yePCR yexesha lokwenyani BioRad CFX Opus 96 Real-Time PCR System |
Ukuhamba komsebenzi
IMacro & Micro-Test General DNA/RNA Kit (HWTS-3019) (enokuthi isetyenziswe kunye neMacro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, (HWTS-3006B)nguJiangsu Macro kunye neMicro-Test Med-Tech Co., Ltd. kucetyiswa ukuba kutsalwe isampulu kunyeamanyathelo alandelayo kufunekaqhubated ngokungqongqo ngokungqinelana ne-IFUyeKit.
