Iintsholongwane Ezintandathu Zokuphefumla
Igama lemveliso
I-HWTS-RT175-Iintsholongwane Ezintandathu Zokuphefumla I-Nucleic Acid Detection Kit (i-Fluorescence PCR)
I-Epidemiology
Izifo zokuphefumla zezona zifo zixhaphakileyo ebantwini ezinokubakho nakweyiphi na isini, ubudala kunye nendawo kwaye zezinye zezona zizathu zibalulekileyo zokugula nokufa kwabantu kwihlabathi liphela. Izifo zokuphefumla eziqhelekileyo ziquka intsholongwane yokuphefumla ehambelanayo, i-adenovirus, i-human metapneumovirus, i-rhinovirus, i-parainfluenza virus (I/II/III) kunye neMycoplasma pneumoniae. Iimpawu zeklinikhi ezibangelwa zizifo zokuphefumla ziyafana, kodwa unyango, ukusebenza kakuhle, kunye nobude besifo ziyahluka phakathi kwezifo ezibangelwa zizifo ezahlukeneyo. Okwangoku, iindlela eziphambili zokufumanisa i-pathogens zokuphefumla ezichazwe apha ngasentla ziquka: ukwahlulwa kwentsholongwane, ukufunyanwa kwe-antigen kunye nokufunyanwa kwe-nucleic acid. Le khithi inceda ekuchongeni izifo zokuphefumla ngokufumana nokuchonga ii-nucleic acids ezithile kubantu abaneempawu zezifo zokuphefumla, kunye nezinye iziphumo zeklinikhi nezelebhu.
Iiparameters zobugcisa
| Indawo yokugcina izinto | ≤-18℃ |
| Beka ubomi kwishelufa | Iinyanga ezili-12 |
| Uhlobo lweSibonelelo | Isampuli ye-Oropharyngeal swab |
| Ct | Adv, PIV, MP, RhV, hMPV, RSV Ct≤38 |
| CV | <5.0% |
| I-LoD | I-LoD ye-Adv, i-MP, i-RSV, i-hMPV, i-RhV kunye ne-PIV zonke ziyi-200Copies/mL |
| Ukuchaneka | Iziphumo zovavanyo lwe-cross-reactivity zibonise ukuba akukho cross-reactivity phakathi kwekhithi kunye ne-coronavirus entsha, i-influenza A virus, i-influenza B virus, i-human bocavirus, i-cytomegalovirus, i-herpes simplex virus type 1, i-varicella zoster virus, i-EBV, i-pertussis bacillus, i-Chlamydophila pneumoniae, i-Corynebacterium spp, i-Escherichia coli, i-Haemophilus influenzae, i-Lactobacillus spp, i-Legionella pneumophila, i-C. catarrhalis, kunye neentlobo ze-Mycobacterium tuberculosis ezincitshisiweyo, i-Neisseria meningitidis, i-Neisseria spp, i-Pseudomonas aeruginosa, i-Staphylococcus aureus, i-Staphylococcus epidermidis, i-Streptococcus pneumoniae, i-Streptococcus pyogenes, i-Streptococcus salivarius, i-Actinobacillus baumannii, i-Narrow-feeding maltophilic monococci, i-Burkholderia maltophilia, i-Streptococcus striatus, i-Nocardia sp., iSarcophaga viscosa, iCitrobacter citriodora, iCryptococcus spp, iAspergillus fumigatus, iAspergillus flavus, iPneumatobacteria spp, iCandida albicans, iRohypnogonia viscera, i-oral streptococci, iKlebsiella pneumoniae, iChlamydia psittaci, iRickettsia q fever kunye ne-human genomic nucleic acids. Amandla okulwa nokuphazamiseka: i-mucin (60 mg/mL), igazi lomntu, i-benfotiamine (2 mg/mL), i-oxymetazoline (2 mg/mL), i-sodium chloride (20 mg/mL), i-beclomethasone (20 mg/mL), i-dexamethasone (20 mg/mL), i-flunitrazolone (20 μg/mL), i-triamcinolone acetonide (2 mg/mL), i-budesonide (1 mg/mL), i-mometasone (2 mg/mL), i-fluticasone (2 mg/mL), i-histamine hydrochloride (5 mg/mL), i-intranasal live influenza virus vaccine, i-benzocaine (10%), i-menthol (10%), i-zanamivir (20 mg/mL), i-ribavirin (10 mg/L), i-paramivir (1 mg/mL), i-oseltamivir (0.15 mg/mL), i-mupirocin (20 mg/mL), i-tobramycin (0.6) mg/mL), i-UTM, i-saline, i-guanidine hydrochloride (5 M/L), i-Tris (2 M/L), i-ENTA-2Na (0.6 M/L), i-trilostane (15%), i-isopropyl alcohol (20%), kunye ne-potassium chloride (1 M/L) ziye zavavanywa ngokuphazamiseka, iziphumo zibonise ukuba akukho kuphazamiseka kwiziphumo zokufunyanwa kwe-pathogen kumanqanaba angentla ezinto eziphazamisayo. |
| Izixhobo Ezisebenzayo | Iinkqubo ze-PCR ze-SLAN-96P zeXesha langempela Inkqubo ye-PCR ye-Applied Biosystems 7500 Real-Time I-QuantStudio®Iinkqubo ezi-5 ze-PCR zeXesha langempela Inkqubo yokuHlola i-PCR yeLineGene 9600 Plus ngexesha langempela I-MA-6000 Real-Time Quantitative Thermal Cycler Inkqubo ye-PCR ye-BioRad CFX96 yeXesha langempela Inkqubo ye-PCR ye-BioRad CFX Opus 96 yeXesha langempela |
Ukuhamba komsebenzi
I-Macro & Micro-Test General DNA/RNA Kit (HWTS-3019) (engasetyenziswa kunye ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, (HWTS-3006B)yiJiangsu Macro kunye neMicro-Test Med-Tech Co., Ltd. ziyacetyiswa xa kutsalwa isampuli kunyeamanyathelo alandelayo kufunekaukuqhubaimiselwe ngokuhambelana ngqongqo ne-IFUyeKit.
