Iindidi ezili-12 zePathogen yokuphefumla

Inkcazelo emfutshane:

Le kiti isetyenziselwa ukufunyaniswa komgangatho odibeneyo we-SARS-CoV-2, intsholongwane yomkhuhlane A, intsholongwane yomkhuhlane B, i-adenovirus, i-mycoplasma pneumoniae, i-rhinovirus, intsholongwane yokuphefumla ye-syncytial virus kunye nentsholongwane ye-parainfluenza (Ⅰ, II, III, IV) kunye ne-metapneumovirus yabantu i-oropharyngeal swabs.

Thumela i-imeyile kuthi

Iinkcukacha zeMveliso

Iithegi zeMveliso

Igama lemveliso

HWTS-RT071A Iindidi ezili-12 zePathogen ye-Nucleic Acid yokuFumana yokuphefumla (i-Fluorescence PCR)

Umjelo

Umjelo	hu12Isithinteli sokuphendula A	hu12Isithinteli sokuphendula B	hu12Isithinteli sokuphendula C	hu12Isithinteli sokuphendula D
FAM	I-SARS-CoV-2	I-HADV	HPIV Ⅰ	HRV
VIC/HEX	Ulawulo lwangaphakathi	Ulawulo lwangaphakathi	I-HPIV Ⅱ	Ulawulo lwangaphakathi
CY5	IFV A	MP	I-HPIV Ⅲ	/
ROX	IFV B	RSV	I-HPIV Ⅳ	HMPV

IiParameters zobuGcisa

Ugcino	≤-18℃
Beka ubomi kwishelufa	Iinyanga ezili-12
Uhlobo loMfanekiso	I-Oropharyngeal swab
Ct	≤38
CV	≤5.0%
LoD	SARS-CoV-2:300 Iikopi/mLintsholongwane yomkhuhlane B:500 Iikopi/mLintsholongwane yomkhuhlane A:500 Iikopi/mL Adenovirus:500 Iikopi/mL mycoplasma pneumoniae:500 Iikopi/mL intsholongwane yokuphefumla ye-syncytial: iikopi ezingama-500/mL, parainfluenza virus (Ⅰ, Ⅱ, Ⅲ, Ⅳ):500 Iikopi/mL Rhinovirus:500 Iikopi/mL I-metapneumovirus yabantu: Iikopi ezingama-500/mL
Ukuchaza ngokuthe ngqo	Uphononongo lwe-cross-reactivity lubonisa ukuba akukho-cross-reactivity phakathi kwale kiti kunye ne-enterovirus A, B, C, D, i-epstein-barr virus, intsholongwane yemasisi, i-cytomegalovirus yabantu, i-rotavirus, i-norovirus, i-mumps virus, i-varicella-herpes zoster virus, bordetella pertussis, streptococcus pyogenes, mycobacterium tuberculosis, aspergillus fumigatus, candida albicans, candida glabrata, pneumocystis jirovecii, cryptococcus neoformans kunye ne-nucleic acid yabantu.
Izixhobo Ezisebenzayo	Iinkqubo ze-Biosystems ezisetyenzisiweyo 7500 Iinkqubo zePCR zeXesha langempelaIinkqubo ze-Biosystems eziSetyenzisiweyo 7500 Iinkqubo zePCR ezikhawulezayoQuantStudio^®IiNkqubo ze-PCR ze-5 zeXesha langempela SLAN-96P Iinkqubo zePCR zeXesha lokwenyani(Hongshi Medical Technology Co., Ltd.) I-LightCycler^®480 inkqubo yePCR yexesha lokwenyani, I-LineGene 9600 kunye neNkqubo yokuFumana i-PCR yexesha lokwenyani (FQD-96A, iteknoloji ye-Hangzhou Bioer) I-MA-6000 yeXesha lokwenyani iBhayisekile yoMyinge weThermal (iSuzhou Molarray Co., Ltd.) I-BioRad CFX96 Inkqubo yePCR yexesha lokwenyani BioRad CFX Opus 96 Real-Time PCR System

Ukuhamba komsebenzi

Ukhetho loku-1.

I-Reagent yokutsalwa ecetyiswayo: I-Macro & Micro-Test yeSampuli yokuKhupha iReagent (HWTS-3005-8) nguJiangsu Macro kunye neMicro-Test Med-Tech Co., Ltd., Ukukhutshwa kufuneka kuqhutywe ngokungqongqo ngokuhambelana nemiyalelo.

Ukhetho lwesi-2.

I-reagent yokutsalwa ecetyiswayo: Macro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) kunye neMacro & Micro-Test Automatic Nucleic Acid Extractor ( HWTS-3006C, HWTS-3006B) nguJiangsu Macro & Micro-Test Med-Tech Co., Ltd., ukukhutshwa kufuneka kuqhutywe ngokwemiyalelo ngokungqongqo.Umthamo we-elution ocetyiswayo yi-80μL.

Ukhetho lwesi-3.

I-reagent yokutsalwa ecetyiswayo: I-Nucleic Acid Extraction okanye i-Purification Kit (YDP315) yi-Tiangen Biotech (Beijing) Co., Ltd., ukukhutshwa kufuneka kuqhutywe ngokwemiyalelo ngokungqongqo.Umthamo we-elution ocetyiswayo yi-100μL.

Ngaphambili: Intsholongwane yeHepatitis E

Okulandelayo: Iintlobo ze-19 ze-Bloodstream Infection Pathogens

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