Iintlobo ezili-12 zePathogen yokuphefumla
Igama lemveliso
I-HWTS-RT071A Iindidi ezili-12 zeKit yokuFumanisa i-Nucleic Acid yePathogen yokuPhefumla (i-Fluorescence PCR)
Itshaneli
| Itshaneli | hu12I-Reaction Buffer A | hu12I-Reaction Buffer B | hu12I-Reaction Buffer C | hu12I-Reaction Buffer D |
| I-FAM | I-SARS-CoV-2 | I-HADV | I-HPIV Ⅰ | I-HRV |
| I-VIC/HEX | Ulawulo lwangaphakathi | Ulawulo lwangaphakathi | I-HPIV Ⅱ | Ulawulo lwangaphakathi |
| CY5 | IFV A | MP | I-HPIV Ⅲ | / |
| I-ROX | IFV B | I-RSV | I-HPIV Ⅳ | I-HMPV |
Iiparameters zobugcisa
| Indawo yokugcina izinto | ≤-18℃ |
| Beka ubomi kwishelufa | Iinyanga ezili-12 |
| Uhlobo lweSibonelelo | I-Oropharyngeal swab |
| Ct | ≤38 |
| CV | ≤5.0% |
| I-LoD | I-SARS-CoV-2: Iikopi ezingama-300/mLIntsholongwane ye-influenza B: Iikopi ezingama-500/mLIntsholongwane ye-influenza A: Iikopi ezingama-500/mL I-Adenovirus: Iikopi ezingama-500/mL i-mycoplasma pneumoniae: Iikopi ezingama-500/mL intsholongwane yokuphefumla ye-syncytial: Iikopi ezingama-500/mL, intsholongwane ye-parainfluenza (Ⅰ, Ⅱ, Ⅲ, Ⅳ): Iikopi ezingama-500/mL I-Rhinovirus: Iikopi ezingama-500/mL i-human metapneumovirus: Iikopi ezingama-500/mL |
| Ukuchaneka | Uphononongo lwe-cross-reactivity lubonisa ukuba akukho cross-reactivity phakathi kwale kit kunye ne-enterovirus A, B, C, D, i-epstein-barr virus, i-measles virus, i-human cytomegalovirus, i-rotavirus, i-norovirus, i-mumps virus, i-varicella-herpes zoster virus, i-bordetella pertussis, i-streptococcus pyogenes, i-mycobacterium tuberculosis, i-aspergillus fumigatus, i-candida albicans, i-candida glabrata, i-pneumocystis jirovecii, i-cryptococcus neoformans kunye ne-human genomic nucleic acid. |
| Izixhobo Ezisebenzayo | Iinkqubo ze-PCR ze-Applied Biosystems 7500 Real-TimeIinkqubo ze-PCR eziSebenzisiweyo ze-7500 ezikhawulezayo zeXesha langempelaI-QuantStudio®Iinkqubo ezi-5 ze-PCR zeXesha langempela Iinkqubo ze-PCR ze-SLAN-96P zeXesha langempela (iHongshi Medical Technology Co., Ltd.) I-LightCycler®Inkqubo ye-PCR yeXesha langempela engama-480, Inkqubo yokuFumanisa i-LineGene 9600 Plus yeXesha langempela (FQD-96A, itekhnoloji ye-Hangzhou Bioer) I-MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) Inkqubo ye-PCR ye-BioRad CFX96 yeXesha langempela Inkqubo ye-PCR ye-BioRad CFX Opus 96 yeXesha langempela |
Ukuhamba komsebenzi
Ukhetho 1.
I-reagent yokukhupha ecetyiswayo: I-Reagent yoKhupho lweSample yeMacro kunye neMicro-Test (HWTS-3005-8) yiJiangsu Macro kunye neMicro-Test Med-Tech Co., Ltd., Ukukhupha kufuneka kwenziwe ngokuhambelana nemiyalelo emiselweyo.
Ukhetho 2.
I-reagent yokukhupha ecetyiswayo: I-Macro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) kunye ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B) yiJiangsu Macro & Micro-Test Med-Tech Co., Ltd., ukukhupha kufuneka kwenziwe ngokwemiyalelo engqongqo. Umthamo wokucoca ocetyiswayo yi-80μL.
Ukhetho 3.
I-reagent yokukhupha ecetyiswayo: I-Nucleic Acid Extraction okanye i-Purification Kit (YDP315) yiTiangen Biotech(Beijing) Co.,Ltd., ukukhupha kufuneka kwenziwe ngokwemiyalelo engqongqo. Umthamo wokucoca ocetyiswayo yi-100μL.
