Iintlobo ezi-4 zeeNtsholongwane zokuphefumla
Igama lemveliso
HWTS-RT099- Iindidi ezi-4 zeeNdlela zokuPhumla zeNucleic Acid Detection Kit (Fluorescence PCR)
I-Epidemiology
Isifo seCorona Virus 2019, esibizwa ngokuba yi "COVID-19", sibhekisa kwinyumoniya ebangelwaI-2019-nCoVusulelo.I-2019-nCoVyi-coronavirus yodidi lwe-β.I-COVID-19 sisifo esosulelayo esinamandla sokuphefumla, kwaye uluntu lusesichengeni ngokubanzi.Okwangoku, umthombo wosulelo ikakhulu zizigulane ezosulelwe yiI-2019-nCoV, kwaye abantu abosulelekileyo abangenazimpawu nabo banokuba ngumthombo wosulelo.Ngokusekelwe kuphando lwangoku lwe-epidemiological, ixesha lokufukamela liyi-1-14 iintsuku, uninzi lweentsuku ezi-3-7.Umkhuhlane, ukukhwehlela okomileyo kunye nokukhathala zizinto eziphambili zokubonakaliswa.Izigulane ezimbalwa zineempawus ezifanaukuxinana kweempumlo, ukuvuza kweempumlo, umqala obuhlungu, i-myalgia kunye norhudo, njl.
Umjelo
| FAM | 2019-nCoV |
| VIC(HEX) | RSV |
| CY5 | IFV A |
| ROX | IFV B |
| NED | Ulawulo lwangaphakathi |
IiParameters zobuGcisa
| Ugcino | -18℃ |
| Beka ubomi kwishelufa | Iinyanga ezili-9 |
| Uhlobo loMfanekiso | I-Oropharyngeal swab |
| Ct | ≤38 |
| LoD | 2019-nCoV: 300Copies/mLIntsholongwane ye-Influenza A/intsholongwane yoMkhuhlane B/intsholongwane yokuphefumla yesyncytial: 500Copies/mL |
| Ukuchaza ngokuthe ngqo | a) Iziphumo ze-cross-reactivity zibonisa ukuba akukho mpendulo emnqamlezweni phakathi kwekhithi kunye ne-coronavirus yabantu i-SARSr-CoV, i-MERSr-CoV, i-HCoV-OC43, i-HCoV-229E, i-HCoV-HKU1, i-HCoV-NL63, uhlobo lwentsholongwane ye-parainfluenza 1, 2, 3, rhinovirus A, B, C, chlamydia pneumoniae, metapneumovirus yabantu, enterovirus A, B, C, D, human pulmonary virus, epstein-barr virus, virus yemasisi, human cytomegalo virus, rotavirus, norovirus, parotitis virus, varicella-zoster intsholongwane, legionella, bordetella pertussis, haemophilus influenzae, staphylococcus aureus, streptococcus pneumoniae, streptococcus pyogenes, klebsiella pneumoniae, mycobacterium tuberculosis, i-aspergillus yomsi, i-candida albicans, i-candida glabrata glabrata ezalwa kunye ne-pneecoccus entsha ye-pneecoccus ye-pneococcus yabantu b) Ikhono lokuchasana nokuphazamiseka: khetha i-mucin (60mg / mL), i-10% (v / v) yegazi kunye ne-phenylephrine (2mg / mL), i-oxymetazoline (2mg / mL), i-sodium chloride (kubandakanywa ne-preservatives) (20 mg / mL) ), i-beclomethasone (20mg/mL), i-dexamethasone (20mg/mL), i-flunisolide (20μg/mL), i-triamcinolone acetonide (2mg/mL), ibudesonide (2mg/mL), i-mometasone (2mg/mL), i-fluticasone (2mg/mL) ), histamine hydrochloride (5mg/mL), alpha interferon (800IU/mL), zanamivir (20mg/mL), ribavirin (10mg/mL), oseltamivir (60ng/mL), peramivir (1mg/mL), lopinavir (500mg/ mL), ritonavir (60mg/mL), mupirocin (20mg/mL), azithromycin (1mg/mL), ceftriaxone (40μg/mL), meropenem (200mg/mL), levofloxacin (10μg/mL) kunye tobramycin (0.6mg/ mL) kuvavanyo lokuphazamiseka, kwaye iziphumo zibonisa ukuba izinto eziphazamisayo ezinoxinzelelo olukhankanywe ngasentla azinampendulo yokuphazamiseka kwiziphumo zovavanyo lwe-pathogens. |
| Izixhobo Ezisebenzayo | Isetyenziselwe i-Biosystems ye-7500 ye-Real-Time PCR System Iinkqubo ze-Biosystems eziSetyenzisiweyo 7500 Iinkqubo zePCR ezikhawulezayo I-QuantStudio®5 Iinkqubo zePCR zeXesha langempela |
Ukuhamba komsebenzi
Ukhetho loku-1.
IMacro & Micro-Test Viral DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) kunye neMacro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006) eveliswe nguJiangsu Macro & Micro -Test Med-Tech Co., Ltd. Umthamo wesampulu ekhutshiweyo ngu-200μL, kunye nomthamo ocetyiswayo we-elution ngu-80μL.
Ukhetho lwesi-2.
I-QIAamp Viral RNA Mini Kit (52904) eveliswe yi-QIAGEN okanye i-Nucleic Acid Extraction okanye i-Purification Kit (YDP315-R) eveliswe yi-Tiangen Biotech (Beijing) Co., Ltd. Umthamo wesampula okhutshiweyo ngu-140μL, kunye nomthamo we-elution ocetyiswayo ngu-60μL.
