Iintlobo ezili-14 ze-HPV Nucleic Acid Typing

Iindidi ezili-14 zeHPV Nucleic Acid Typing Umfanekiso obonakalayo

Inkcazelo emfutshane:

I-Human Papillomavirus (HPV) yeyosapho lwe-Papillomaviridae ye-molecule encinci, engaguqukiyo, i-circular double-stranded DNA virus, kunye nobude be-genome malunga ne-8000 base pairs (bp). I-HPV yosulela abantu ngokudibana ngokuthe ngqo okanye ngokungangqalanga nezinto ezisulelekileyo okanye usulelo lwesondo. Intsholongwane ayikho nje i-host-specific, kodwa i-tissue-specific, kwaye inokosulela kuphela ulusu lomntu kunye neeseli ze-epithelial ze-mucosal, ezibangela iindidi ze-papillomas okanye iintsumpa eluswini lomntu kunye nomonakalo okhulayo kwi-epithelium yendlela yokuzala.

Ikhithi ilungele ukufunyaniswa kokuchwetheza okusemgangathweni kwe-in vitro kwiindidi ezili-14 ze-papillomaviruses yabantu (HPV16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, 68) isampulu ye-nucleic yomchamo kunye nesampulu yomchamo yabantu ababhinqileyo. iisampuli swab. Inokubonelela kuphela ngeendlela ezincedisayo zokuxilonga kunye nonyango losulelo lwe-HPV.

Thumela i-imeyile kuthi

Iinkcukacha zeMveliso

Iithegi zeMveliso

Igama lemveliso

I-HWTS-CC012A-14 Iintlobo ze-HPV Nucleic Acid Typing Kit (Fluorescence PCR)

HWTS-CC021-Umkhenkce-zomisiweyo ezili-14 Iintlobo ze-Human Papillomavirus Nucleic Acid Typing Detection Kit (Fluorescence PCR)

Isatifikethi

I-Epidemiology

Umhlaza womlomo wesibeleko lelinye lawona mathumba ayingozi axhaphakileyo kwindlela yokuzala yabasetyhini. Uphononongo lubonise ukuba usulelo oluqhubekayo kunye nosulelo oluninzi lwe-human papillomavirus yenye yezizathu ezibalulekileyo zomhlaza womlomo wesibeleko. Okwangoku, kusekho ukunqongophala kweendlela zonyango ezisebenzayo ze-HPV. Ke ngoko, ukufunyaniswa kwangoko kunye nokuthintelwa kwangoko kwe-HPV yomlomo wesibeleko ngundoqo ekuthinteleni umhlaza. Ukusekwa kwendlela yokuxilonga i-pathogenic elula, ecacileyo kunye ekhawulezayo ibaluleke kakhulu ekuxilongweni kweklinikhi yomhlaza womlomo wesibeleko.

Umjelo

FAM	I-HPV16, i-58, isalathiso sangaphakathi
VIC(HEX)	HPV18, 33, 51, 59
CY5	HPV35, 45, 56, 68
ROX	HPV31, 39, 52, 66

Iiparamitha zobuGcisa

Ugcino	≤-18℃ ebumnyameni
Beka ubomi kwishelufa	Iinyanga ezili-12
Uhlobo loMfanekiso	Umchamo, i-Servical swab, i-swab yelungu lobufazi
Ct	≤28
CV	<5.0%
LoD	300Iikopi/mL
Izixhobo Ezisebenzayo	Iyakwazi ukuhambelana nezixhobo zePCR zefluorescent kwimarike.SLAN-96P Iinkqubo zePCR zeXesha langempela Iinkqubo ze-Biosystems ezisetyenzisiweyo 7500 Iinkqubo zePCR zeXesha langempela QuantStudio^®IiNkqubo ze-PCR ze-5 zeXesha langempela I-LightCycler^®Inkqubo ye-PCR ye-480 yexesha langempela I-LineGene 9600 Plus Real-Time PCR Detection System MA-6000 Real-Time Quantitative Thermal Cycler