Iintlobo ezili-14 ze-HPV Nucleic Acid Typing

Inkcazo emfutshane:

I-Human Papillomavirus (HPV) ikwintsapho yePapillomaviridae yentsholongwane ye-DNA encinci, engagqunywanga, ejikelezayo enemicu emibini, enobude be-genome obumalunga ne-8000 base pairs (bp). I-HPV yosulela abantu ngokudibana ngqo okanye ngokungathanga ngqo nezinto ezingcolisiweyo okanye ukudluliselwa ngokwesondo. Le ntsholongwane ayijoliswanga nje kuphela kumninimzi, kodwa ikwajoliswa nakwizicubu ezithile, kwaye inokosulela kuphela ulusu lomntu kunye neeseli ze-epithelial ze-mucosal, nto leyo ebangela iintlobo ngeentlobo zee-papillomas okanye ii-warts eluswini lomntu kunye nomonakalo okhulayo kwi-epithelium yokuzala.

Le khithi ifanelekile ekuchongweni kwe-in vitro qualitative typing kwiintlobo ezili-14 ze-human papillomaviruses (HPV16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, 68) nucleic acids kwiisampuli zomchamo womntu, iisampuli ze-cervical swab yabasetyhini, kunye neesampuli ze-vaginal swab yabasetyhini. Inokubonelela kuphela ngeendlela ezincedisayo zokuxilonga kunye nonyango losulelo lwe-HPV.

Thumela i-imeyile kuthi

Iinkcukacha zeMveliso

Iithegi zeMveliso

Igama lemveliso

Iintlobo ze-HWTS-CC012A-14 ze-HPV Nucleic Acid Typing Detection Kit (Fluorescence PCR)

I-HWTS-CC021-Iintlobo ezili-14 zeHuman Papillomavirus Nucleic Acid Typing Kit (Fluorescence PCR)

Isatifikethi

I-Epidemiology

Umhlaza womlomo wesibeleko yenye yezona zifo zixhaphakileyo kwindlela yokuzala yabasetyhini. Izifundo zibonise ukuba usulelo oluqhubekayo kunye nosulelo oluninzi lwe-human papillomavirus yenye yezona zinto zibalulekileyo zomhlaza womlomo wesibeleko. Okwangoku, kusekho ukunqongophala kweendlela zonyango ezisebenzayo ze-HPV. Ke ngoko, ukufunyaniswa kwangoko kunye nokuthintela kwangoko i-HPV yomlomo wesibeleko yeyona nto iphambili ekuthinteleni umhlaza. Ukusekwa kwendlela yokuxilonga i-pathogenic elula, ethe ngqo nekhawulezayo kubaluleke kakhulu ekuxilongweni komhlaza womlomo wesibeleko.

Itshaneli

I-FAM	I-HPV16, 58, isalathiso sangaphakathi
I-VIC(HEX)	I-HPV18, 33, 51, 59
CY5	I-HPV35, 45, 56, 68
I-ROX	I-HPV31, 39, 52, 66

Iiparameters zobugcisa

Indawo yokugcina izinto	≤-18℃ Ebumnyameni
Beka ubomi kwishelufa	Iinyanga ezili-12
Uhlobo lweSibonelelo	Umchamo, iswabhu yomlomo wesibeleko, iswabhu yangaphantsi
Ct	≤28
CV	<5.0%
I-LoD	Iikopi ezingama-300/mL
Izixhobo Ezisebenzayo	Ingalingana nezixhobo zePCR ezikhanyayo ezithengiswayo.Iinkqubo ze-PCR ze-SLAN-96P zeXesha langempela Iinkqubo ze-PCR ze-Applied Biosystems 7500 Real-Time I-QuantStudio^®Iinkqubo ezi-5 ze-PCR zeXesha langempela I-LightCycler^®Inkqubo ye-PCR yeXesha langempela ye-480 Inkqubo yokuHlola i-PCR yeLineGene 9600 Plus ngexesha langempela I-MA-6000 Real-Time Quantitative Thermal Cycler