Ikhithi yokuFumanisa iTrichomonas Vaginalis Nucleic Acid (iFluorescence PCR)

Umfanekiso obonisiweyo weTrichomonas Vaginalis Nucleic Acid Detection Kit (Fluorescence PCR)

Inkcazo emfutshane:

Le khithi isetyenziselwa ukufumanisa umgangatho we-Trichomonas vaginalis nucleic acid kwiisampuli zokukhupha ulwelo kwindlela yokuzala yabantu.

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Ikhithi yokuFumanisa iTrichomonas Vaginalis Nucleic Acid (iFluorescence PCR)

I-Epidemiology

I-Trichomonas vaginalis (TV) sisifo esibangela i-flagellate kwi-vagina yomntu nakwi-urinary tract, nto leyo ebangela i-trichomonas vaginitis kunye ne-urethritis, kwaye sisifo esosulelayo esidluliselwa ngesondo. I-Trichomonas vaginalis iyakwazi ukuziqhelanisa kakhulu nokusingqongileyo kwangaphandle, kwaye uninzi lwabantu luchaphazeleka lula. Kukho abantu abamalunga nezigidi ezili-180 abasulelekileyo kwihlabathi liphela, kwaye izinga losulelo liphezulu phakathi kwabasetyhini abaneminyaka engama-20 ukuya kwengama-40. Usulelo lwe-Trichomonas vaginalis lunokunyusa amathuba okuba ne-human immunodeficiency virus (HIV), i-human papillomavirus (HPV), njl. Uphando olukhoyo lubonisa ukuba usulelo lwe-Trichomonas vaginalis lunxulumene kakhulu nokukhulelwa okubi, i-cervicitis, ukungazali, njl. njl., kwaye lunxulumene nokubakho kunye nokubikezela kwe-tumor enobungozi yendlela yokuzala efana nomhlaza wesibeleko, umhlaza we-prostate, njl. njl. Ukuxilongwa ngokuchanekileyo kosulelo lwe-Trichomonas vaginalis likhonkco elibalulekileyo ekuthinteleni nasekunyangweni kwesi sifo, kwaye kubaluleke kakhulu ukuthintela ukusasazeka kwesi sifo.

Itshaneli

I-FAM	I-asidi ye-nucleic yeTV
I-VIC(HEX)	Ulawulo lwangaphakathi

Iiparameters zobugcisa

Indawo yokugcina izinto	Ulwelo: ≤-18℃ Ebumnyameni
Beka ubomi kwishelufa	Iinyanga ezili-12
Uhlobo lweSibonelelo	ukukhupha umchamo, ukukhupha komlomo wesibeleko
Ct	≤38
CV	＜5.0%
I-LoD	Iikopi ezingama-400/mL
Ukuchaneka	Akukho kuphinda kusebenze kunye nezinye iisampuli zephecana le-urogenital, ezifana neCandida albicans, iChlamydia trachomatis, iUreaplasma urealyticum, iNeisseria gonorrhoeae, iQela B streptococcus, Mycoplasma hominis, Mycoplasma genitalium, Herpes simplex virus, Human papillomachidnervavirus, varlachidnervavirus, i-varian papillomavirus, i-varian coliculischerischeria i-staphylococcus aureus kunye ne-Human Genomic DNA, njl.
Izixhobo Ezisebenzayo	Ingalingana nezixhobo zePCR ezikhanyayo ezithengiswayo. Iinkqubo ze-PCR eziSebenzisiweyo ze-7500 ezikhawulezayo zeXesha langempela Iinkqubo ze-PCR zeQuantStudio®5 zeXesha langempela Iinkqubo ze-PCR ze-SLAN-96P zeXesha langempela Inkqubo ye-PCR yeLightCycler®480 yeXesha langempela Inkqubo yokuHlola i-PCR yeLineGene 9600 Plus ngexesha langempela I-MA-6000 Real-Time Quantitative Thermal Cycler Inkqubo ye-PCR ye-BioRad CFX96 yeXesha langempela Inkqubo ye-PCR ye-BioRad CFX Opus 96 yeXesha langempela

Ukuhamba komsebenzi

I-reagent iyafuneka kodwa ayibonelelwanga

I-Macro & Micro-Test General DNA/RNA Kit (HWTS-3019-50, HWTS-3019-32, HWTS-3019-48, HWTS-3019-96) (engasetyenziswa kunye ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) yiJiangsu Macro & Micro-Test Med-Tech Co., Ltd., Nucleic Acid Extraction okanye Purification Kit (YDP302).

Izinto ezisetyenziswayo ziyafuneka kodwa azibonelelwanga

I-1.5mL yeetyhubhu ze-centrifuge ezingenayo i-RNase/DNase, ii-tips ezingenayo i-RNase/DNase, i-0.2mL yeetyhubhu ze-PCR reaction, i-benchtop centrifuge, i-benchtop shaker mixer

Ngaphambili: Ikhithi yokuFumanisa iChlamydia Trachomatis Nucleic Acid (iFluorescence PCR)

Okulandelayo: Iintlobo ezili-14 ze-HPV Nucleic Acid Typing

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