Iindidi ezingama-28 zeVilloma Emngciphekweni omkhulu we-papilloma (16/18 Thaiping) i-nucleic acid
Igama lemveliso
I-HWTS-CC006A-28 Iindidi ze-Papilloma enomngcipheko omkhulu (i-16/16 yotywala) i-tycleciacciac yokufumana i-nucleid ikhithi
Isipikumu
Umhlaza womlomo wesibeleko yenye yezona zinto zixhaphakileyo zeThutyhefu yokuzala. Izifundo zibonakalise ukuba usulelo oluqhubekayo lwe-HPV kwaye usulelo oluninzi yenye yezizathu eziphambili zomhlaza womlomo wesibeleko. Okwangoku, unyango olusebenzayo olusebenzayo lusasilela kumhlaza womlomo wesibeleko obangelwe yi-HPV, ukufumanisa ukufunyanwa kwakhona kunye nokuthintela usulelo lwesibeleko olubangelwa yi-HPV sisitshixo sokuthintela umhlaza wesibeleko. Kubaluleke kakhulu ukuseka uvavanyo olulula, oluthile kwaye lukhawulezile kwaye lukhawulezile ukufunyanwa kwesifo sekliniki kunye nonyango lomhlaza womlomo wesibeleko.
Ijelo
| Ukuxuba kwempendulo | Ijelo | Uhlobo |
| I-PCR-xube1 | Indlala | 18 |
| Vic (hex) | 16 | |
| Rox | 31, 33, 35, 35, 55, 52, 52, 58, 58, 68, 68 | |
| I-Cy5 | Ulawulo lwangaphakathi | |
| I-PCR-xube2 | Indlala | 6, 11, 54, 83 |
| Vic (hex) | 26, 44, 61, 81 | |
| Rox | 40, 42, 43, 53, 73, 82 | |
| I-Cy5 | Ulawulo lwangaphakathi |
Iiparamitha zobugcisa
| Ukugcinwa | Ulwelo: ≤-18 ℃ |
| Beka ubomi kwishelufa | Iinyanga ezili-12 |
| Uhlobo locaphu | I-wirvical swab, i-vaginal swab, umchamo |
| Ct | ≤28 |
| CV | <5.0% |
| Indawo yokuhlala | I-300copies / ml |
| Izixhobo ezifanelekileyo | Faka isicelo se-biosystemsts 700 ye-PCR ye-PCR ye-PCR Faka isicelo se-biosystemsts ezingama-700 ngokukhawuleza kwe-PCR ye-PCR Ubungakanani®Iinkqubo ezi-5 zokwenyani ze-PCR I-Slan-96p yangempela ye-PCR ye-PCR Ukukhanya®I-480 yeyokwenene ye-PCR I-Linegene 9600 kunye neNkqubo yokufumana iPRRR Ma-6000 ubungakanani bexesha lokwenyani I-biorad cfx96 yeyokwenyani ye-PFRR I-biorad cfx opi 96 Inkqubo ye-PFR ye-PCR |
Ukuhamba komsebenzi
Ukucetyiswa Ukuqulunqwa kwe-Reagent: I-Macro kunye ne-Macro kunye ne-RNA Kit (HWTS-3017-50, HWTS-3017-7-53, HWTS-3017-56) Isikhupheli se-nucleic esizenzekelayo (i-HWTS-3006C, HWTS-3006B)) nguJiangsu Macro & I-Micro-Test Count-Med-Tex Count-Tex Co.ine-LTD. Yongeza i-Suline eqhelekileyo ukuze urhoxise i-pellet kwinyathelo 2.1, kwaye emva koko ukuthunyelwa kufuneka kwenziwe ngokwemiyalelo yokuqalisa. Ivolumu yesiqhelo yesiqhelo yi-80μl.
Ukucetyiswa Ukuqhekeza Ukuphinda Usebenze: I-Qiaamp DNA Mini Kit ikhithi (i-51304) okanye i-Macro kunye ne-Macro kunye neKholamu ye-DNA / i-HWS-3020-50). Yongeza i-2000l ye-saline yesiqhelo ukuba iphinde iphinde iphinde iphinde i-pellet kwinyathelo 2.1, kwaye ke ukukhutshwa kufuneka kwenziwe ngokwemiyalelo yokusetyenziswa kolu sugent. Ivoramu yesampulu ekhutshiweyo ye-200μl, kwaye ivolumu yesiqhelo ye-Eprelin yi-100μl.
