Iintlobo ezingama-28 ze-Human Papilloma Virus ezinobungozi obuphezulu (16/18 Typing) Nucleic Acid
Igama lemveliso
Iintlobo ze-HWTS-CC006A-28 ze-Human Papilloma Virus enobungozi obuphezulu (16/18 Typing) I-Nucleic Acid Detection Kit (i-Fluorescence PCR)
I-Epidemiology
Umhlaza womlomo wesibeleko yenye yezona zifo zixhaphakileyo kwindlela yokuzala yabasetyhini. Izifundo zibonise ukuba usulelo oluqhubekayo lwe-HPV kunye nosulelo oluninzi zezinye zezona zizathu ziphambili zomhlaza womlomo wesibeleko. Okwangoku, unyango olusebenzayo oluqatshelweyo alusekho kumhlaza womlomo wesibeleko obangelwa yi-HPV, ngoko ke ukufunyanwa kwangoko kunye nokuthintela usulelo lomlomo wesibeleko olubangelwa yi-HPV yeyona nto iphambili ekuthinteleni umhlaza womlomo wesibeleko. Kubaluleke kakhulu ukumisela uvavanyo olulula, olucacileyo nolukhawulezayo lokuxilonga i-etiology yokuxilongwa kweklinikhi kunye nonyango lomhlaza womlomo wesibeleko.
Itshaneli
| Umxube weReaction | Itshaneli | Uhlobo |
| I-PCR-Mix1 | I-FAM | 18 |
| I-VIC(HEX) | 16 | |
| I-ROX | 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, 68 | |
| CY5 | Ulawulo lwangaphakathi | |
| I-PCR-Mix2 | I-FAM | 6, 11, 54, 83 |
| I-VIC(HEX) | 26, 44, 61, 81 | |
| I-ROX | 40, 42, 43, 53, 73, 82 | |
| CY5 | Ulawulo lwangaphakathi |
Iiparameters zobugcisa
| Indawo yokugcina izinto | Ulwelo: ≤-18℃ |
| Beka ubomi kwishelufa | Iinyanga ezili-12 |
| Uhlobo lweSibonelelo | I-Swab yoMlomo, I-Swab yoBufazi, Umchamo |
| Ct | ≤28 |
| CV | <5.0% |
| I-LoD | Iikopi ezingama-300/mL |
| Izixhobo Ezisebenzayo | Iinkqubo ze-PCR ze-Applied Biosystems 7500 Real-Time Iinkqubo ze-PCR eziSebenzisiweyo ze-7500 ezikhawulezayo zeXesha langempela I-QuantStudio®Iinkqubo ezi-5 ze-PCR zeXesha langempela Iinkqubo ze-PCR ze-SLAN-96P zeXesha langempela I-LightCycler®Inkqubo ye-PCR yeXesha langempela ye-480 Inkqubo yokuHlola i-PCR yeLineGene 9600 Plus ngexesha langempela I-MA-6000 Real-Time Quantitative Thermal Cycler Inkqubo ye-PCR ye-BioRad CFX96 yeXesha langempela Inkqubo ye-PCR ye-BioRad CFX Opus 96 yeXesha langempela |
Ukuhamba komsebenzi
I-reagent yokukhupha ecetyiswayo: I-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (engasetyenziswa kunye ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) yiJiangsu Macro & Micro-Test Med-Tech Co., Ltd. Yongeza i-200μL ye-saline eqhelekileyo ukuze uphinde umise i-pellet kwinyathelo lesi-2.1, kwaye emva koko ukukhupha kufuneka kwenziwe ngokwemiyalelo yokusebenzisa le reagent yokukhupha. Umthamo wokucoca ocetyiswayo yi-80μL.
I-reagent yokukhupha ecetyiswayo: I-QIAamp DNA Mini Kit (51304) okanye i-Macro & Micro-Test Viral DNA/RNA Column (HWTS-3020-50). Yongeza i-200μL ye-saline eqhelekileyo ukuze uphinde umise i-pellet kwinyathelo lesi-2.1, uze emva koko ukukhupha kufuneka kwenziwe ngokwemiyalelo yokusebenzisa le reagent yokukhupha. Umthamo wesampulu ekhutshiweyo weesampuli zonke ziyi-200μL, kwaye umthamo wokucoca ocetyiswayo yi-100μL.
