Iindidi ezingama-29 zeePathogens zokuphefumla eziDityanisiweyo zeNucleic Acid
Igama lemveliso
I-HWTS-RT160 -29 Iindidi zeePathogens zokuphefumla eziDityanisiweyo ze-Nucleic Acid Detection Kit
I-Epidemiology
Usulelo lwendlela yokuphefumla sesona sifo sixhaphakileyo ebantwini, esinokuthi senzeke nakwesiphi na isini, ubudala kunye nommandla. Ngomnye woonobangela abaphambili bokugula nokufa kubemi behlabathi[1]. Iintsholongwane zokuphefumla eziqhelekileyo ziquka inoveli coronavirus, intsholongwane yoMkhuhlane A, intsholongwane yoMkhuhlane B, intsholongwane yokuphefumla syncytial virus, Adenovirus, metapneumovirus yabantu, rhinovirus, Parainfluenza virus uhlobo I/II/III, Bocavirus, Enterovirus, Coronavirus, Mycoplasma pneumoniae, Chlamydia pneumonia, etc pneumoniae, Streptococcus Iimpawu kunye neempawu ezibangelwa zizifo zokuphefumula ziyafana, kodwa iindlela zonyango, ukusebenza kakuhle kunye nekhosi yezifo ezibangelwa zizifo ezahlukeneyo zihluke [4,5]. Okwangoku, iindlela eziphambili ezisetyenziswa kwiilabhoratri zokubona ezi zintsholongwane zokuphefumla zikhankanywe ngasentla ziquka: ukwahlukaniswa kwentsholongwane, ukubhaqwa kwe-antigen kunye nobhaqo lwe-nucleic acid, njl. njl Iziphumo ezibi azibandakanyi ukusuleleka yintsholongwane yokuphefumula kwaye akufanele isetyenziswe njengesiseko sodwa sokuxilongwa, unyango, okanye ezinye izigqibo zokulawula. Isiphumo esihle asikwazi ukukhupha usulelo lwebhaktheriya okanye izifo ezixubileyo ngezinye iintsholongwane ezingaphandle kwezibonakaliso zokuvavanya. Abaqhubi abavavanyayo kufanele ukuba bafumene uqeqesho lobuchwephesha ekukhuliseni imizila yemfuza okanye ekubhaqweni kwebhayoloji yemolekyuli, kwaye baneziqinisekiso ezifanelekileyo zokusebenza zovavanyo. Ilabhoratri kufuneka ibe neendawo ezifanelekileyo zokuthintela ukhuseleko lwendalo kunye neenkqubo zokhuseleko.
Iiparamitha zobuGcisa
| Ugcino | -18℃ |
| Beka ubomi kwishelufa | Iinyanga ezili-9 |
| Uhlobo loMfanekiso | Ukuswabha komqala |
| Ct | ≤38 |
| CV | <5.0% |
| LoD | 200Iikopi/μL |
| Ukuchaza ngokuthe ngqo | Iziphumo zovavanyo lwe-cross-reactivity zibonise ukuba akukho mpendulo phakathi kwale kit kunye ne-Cytomegalovirus, i-Herpes simplex virus type 1, i-Varicella-zoster virus, i-virus ye-Epstein-Barr, i-Pertussis, i-Corynebacterium, i-Escherichia coli, i-Haemophilus influenzae, i-Lactobacillus, i-Legionella pneumostaruxelles, i-Legionella pneumostaruxels isifo sephepha, iNeisseria meningitidis, Neisseria, Pseudomonas aeruginosa, Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pyogenes, Streptococcus salivarius, Acinetobacter baumannii, Stenotrophophiliacemonas malkhoummalia, Burkhoococcus malkhoumonia striatum, Nocardia, Serratia marcescens, Citrobacter, Cryptococcus, Aspergillus fumigatus, Aspergillus flavus, Pneumocystis jiroveci, Candida albicans, Rothia mucilaginosus, Streptococcus oralis, Klebsiella pneumoniae, Chlaciidia inyumoniya, iColamydia, iColamydia, iChlamydia, iChlamydia, iChlamydia iiasidi. |
| Izixhobo Ezisebenzayo | Iinkqubo ze-Biosystems ezisetyenzisiweyo 7500 Iinkqubo zePCR zeXesha langempela, Iinkqubo ze-Biosystems eziSetyenzisiweyo 7500 Iinkqubo zePCR ezikhawulezayo, QuantStudio®Iinkqubo zePCR ezi-5 zeXesha langempela, SLAN-96P Iinkqubo zePCR zeXesha lokwenyani (Hongshi Medical Technology Co., Ltd.), I-LightCycler®480 inkqubo yePCR yexesha lokwenyani, I-LineGene 9600 Plus Iinkqubo zokuFumana ze-PCR ngexesha langempela (i-FQD-96A, iteknoloji ye-Hangzhou Bioer), I-MA-6000 yeXesha lokwenyani iBhayisekile yoMyinge weThermal (iSuzhou Molarray Co., Ltd.), Inkqubo yePCR yeBioRad CFX96 yeXesha lokwenyani, BioRad CFX Opus 96 Real-Time PCR System. |
Ukuhamba komsebenzi
IMacro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (enokuthi isetyenziswe kunye neMacro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)), kunye neMacro & Micro-Test Viral DNA/RNA Kit (HWTS-17u-30)TM I-AIO800 (HWTS-EQ007)) yi-Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.
Umthamo wesampulu ekhutshiweyo yi-200μL kwaye umthamo we-elution ocetyiswayo yi-150μL.
