Iintlobo ezi-4 zeeNtsholongwane zokuphefumla
Igama lemveliso
HWTS-RT099- Iindidi ezi-4 zeeNdlela zokuphefumla zeNucleic Acid Detection Kit (Fluorescence PCR)-内参是NED-ABI 7500 iNkqubo yePCR yexesha elililo/ABI 7500 Fast Real-Time PCR Systems/QuantStudio®IiNkqubo ze-PCR ze-5 zeXesha langempela
I-HWTS-RT158-4 Iindidi zeVirusi eziPhumlayo kwiNucleic Acid Detection Kit (Fluorescence PCR)-内参Quasar 705
I-Epidemiology
Isifo seCorona Virus 2019, esibizwa ngokuba yi "COVID-19", sibhekisa kwinyumoniya ebangelwaI-2019-nCoVusulelo.I-2019-nCoVyi-coronavirus yodidi lwe-β. I-COVID-19 sisifo esosulelayo esinamandla sokuphefumla, kwaye uluntu lusesichengeni ngokubanzi. Okwangoku, umthombo wosulelo ikakhulu zizigulane ezosulelwe yiI-2019-nCoV, kwaye abantu abosulelekileyo abangenazimpawu nabo banokuba ngumthombo wosulelo. Ngokusekelwe kuphando lwangoku lwe-epidemiological, ixesha lokufukamela liyi-1-14 iintsuku, uninzi lweentsuku ezi-3-7. Umkhuhlane, ukukhwehlela okomileyo kunye nokukhathala zizinto eziphambili zokubonakaliswa. Izigulane ezimbalwa zineempawus ezifanaukuxinana kweempumlo, ukuvuza kweempumlo, umqala obuhlungu, i-myalgia kunye norhudo, njl.
Umjelo
| FAM | 2019-nCoV |
| VIC(HEX) | RSV |
| CY5 | IFV A |
| ROX | IFV B |
| NED | Ulawulo lwangaphakathi |
Iiparamitha zobuGcisa
| Ugcino | -18℃ |
| Beka ubomi kwishelufa | Iinyanga ezili-9 |
| Uhlobo loMfanekiso | I-Oropharyngeal swab |
| Ct | ≤38 |
| LoD | 2019-nCoV: 300Copies/mLIntsholongwane ye-Influenza A/intsholongwane yoMkhuhlane B/intsholongwane yokuphefumla yesyncytial: 500Copies/mL |
| Ukuchaza ngokuthe ngqo | a) Iziphumo ze-cross-reactivity zibonisa ukuba akukho mpendulo emnqamlezweni phakathi kwekhithi kunye ne-coronavirus yabantu i-SARSr-CoV, i-MERSr-CoV, i-HCoV-OC43, i-HCoV-229E, i-HCoV-HKU1, i-HCoV-NL63, uhlobo lwentsholongwane ye-parainfluenza 1, 2, 3, i-rhinovirus A, B, inyumoniya, i-humanmymochlavirus, i-enternapneuvirus A, B, C, D, intsholongwane yemiphunga yomntu, intsholongwane ye-epstein-barr, intsholongwane yemasisi, intsholongwane ye-cytomegalo yabantu, i-rotavirus, i-norovirus, i-parotitis virus, i-varicella-zoster virus, i-legionella, i-bordetella pertussis, i-haemophilus influenzae, i-staphylococcus aureus, i-streptocuscoccus genesisptococcus, inyumoniya, isifo sephepha se-mycobacterium, i-aspergillus yomsi, i-candida albicans, i-candida glabrata, i-pneumocystis jiroveci kunye ne-cryptococcus esanda kuzalwa kunye ne-nucleic acid yabantu. b) Ikhono lokuchasana nokuphazamiseka: khetha i-mucin (60mg / mL), i-10% (v / v) yegazi kunye ne-phenylephrine (2mg / mL), i-oxymetazoline (2mg / mL), i-sodium chloride (kubandakanywa ne-preservatives) (20 mg / mL), i-beclomethasone (20mg / mL), i-dexamethasone (20mg / mL) (20μg/mL), triamcinolone acetonide (2mg/mL), budesonide (2mg/mL), mometasone (2mg/mL), i-fluticasone (2mg/mL), i-histamine hydrochloride (5mg/mL), i-alpha interferon (800IU/mL), i-zanamimvirim/mL), i-zanamimvirim/i-rimg ye-rimg, i-rimg, i-rimg, i-10ba img (60ng/mL), peramivir (1mg/mL), lopinavir (500mg/mL), ritonavir (60mg/mL), mupirocin (20mg/mL), azithromycin (1mg/mL), ceftriaxone (40μg/mL), meropenem (200mg/mL), levofloxamy (10mg/mL), levofloxamy (0.6mg / mL) uvavanyo lokuphazamiseka, kwaye iziphumo zibonisa ukuba izinto eziphazamisayo ezinogxininiso ezikhankanywe ngasentla azikho ukusabela kokuphazamiseka kwiziphumo zovavanyo lwe-pathogens. |
| Izixhobo Ezisebenzayo | Isetyenziselwe i-Biosystems ye-7500 ye-Real-Time PCR SystemIinkqubo ze-Biosystems eziSetyenzisiweyo 7500 Iinkqubo zePCR ezikhawulezayo I-QuantStudio®5 Iinkqubo zePCR zeXesha langempela |
Ukuhamba komsebenzi
Ukhetho loku-1.
IMacro & Micro-Test Viral DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) kunye neMacro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006) eveliswe nguJiangsu Macro & Micro-Test Coed umthamo we-elution ocetyiswayo yi-80μL.
Ukhetho lwesi-2.
I-QIAamp Viral RNA Mini Kit (52904) eveliswe yi-QIAGEN okanye i-Nucleic Acid Extraction okanye i-Purification Kit (YDP315-R) eveliswe yi-Tiangen Biotech (Beijing) Co., Ltd. Umthamo wesampula okhutshiweyo ngu-140μL, kunye nomthamo we-elution ocetyiswayo ngu-60μL.
