Iintlobo ezi-4 zeentsholongwane zokuphefumla
Igama lemveliso
I-HWTS-RT099- Iintlobo ezi-4 zeentsholongwane zokuphefumla I-Nucleic Acid Detection Kit (Fluorescence PCR)-Inkqubo ye-NED-ABI 7500 Real-Time PCR/Iinkqubo ze-PCR ze-ABI 7500 ezikhawulezayo zeXesha langempela/iQuantStudio®Iinkqubo ezi-5 ze-PCR zeXesha langempela
Iintlobo ze-HWTS-RT158-4 zeentsholongwane zokuphefumla Isixhobo sokubona i-Nucleic Acid (i-Fluorescence PCR)-内参Quasar 705
I-Epidemiology
Isifo seCorona Virus sika-2019, esibizwa ngokuba yi-"COVID-19", sibhekisa kwi-pneumonia ebangelwa yi-I-2019-nCoVusulelo.I-2019-nCoVyintsholongwane ye-coronavirus ekwi-β genus. I-COVID-19 sisifo esosulelayo esihlasela ukuphefumla ngokukhawuleza, kwaye uluntu ngokubanzi lusengozini. Okwangoku, umthombo wosulelo ikakhulu zizigulane ezosulelwe yiI-2019-nCoV, kwaye abantu abanesi sifo abangazibonakalisi iimpawu nabo banokuba ngumthombo wosulelo. Ngokusekelwe kuphando lwangoku lwe-epidemiological, ixesha lokufukama liyi-1-14 yeentsuku, uninzi lwazo luyi-3-7 yeentsuku. Umkhuhlane, ukukhwehlela okomileyo kunye nokudinwa zezona mpawu ziphambili. Izigulana ezimbalwa zazineempawu.ezifana neukuxinana kwempumlo, impumlo egobhozayo, umphimbo obuhlungu, i-myalgia kunye norhudo, njl. njl..
Itshaneli
| I-FAM | 2019-nI-CoV |
| I-VIC(HEX) | I-RSV |
| CY5 | IFV A |
| I-ROX | IFV B |
| I-NED | Ulawulo lwangaphakathi |
Iiparameters zobugcisa
| Indawo yokugcina izinto | -18℃ |
| Beka ubomi kwishelufa | Iinyanga ezi-9 |
| Uhlobo lweSibonelelo | I-Oropharyngeal swab |
| Ct | ≤38 |
| I-LoD | 2019-nCoV: Iikopi ezingama-300/mLIntsholongwane ye-Influenza A/Intsholongwane ye-Influenza B/Intsholongwane ye-Respiratory syncytial: 500Iikopi/mL |
| Ukuchaneka | a) Iziphumo ze-cross-reactivity zibonisa ukuba akukho mpendulo idibeneyo phakathi kwekhithi kunye ne-human coronavirus SARSr-CoV, MERSr-CoV, HCoV-OC43, HCoV-229E, HCoV-HKU1, HCoV-NL63, i-parainfluenza virus type 1, 2, 3, rhinovirus A, B, C, chlamydia pneumoniae, human metapneumovirus, enterovirus A, B, C, D, human pulmonary virus, epstein-barr virus, measles virus, human cytomegalo virus, rotavirus, norovirus, parotitis virus, varicella-zoster virus, legionella, bordetella pertussis, haemophilus influenzae, staphylococcus aureus, streptococcus pneumoniae, streptococcus pyogenes, klebsiella pneumoniae, mycobacterium tuberculosis, smoke aspergillus, candida albicans, candida glabrata, pneumocystis jiroveci kunye ne-cryptococcus esandul' ukuzalwa kunye ne-human genomic nucleic acid. b) Amandla okulwa nokuphazamiseka: i-select mucin (60mg/mL), i-10% (v/v) yegazi kunye ne-phenylephrine (2mg/mL), i-oxymetazoline (2mg/mL), i-sodium chloride (kuquka nezigcini) (20 mg/mL), i-beclomethasone (20mg/mL), i-dexamethasone (20mg/mL), i-flunisolide (20μg/mL), i-triamcinolone acetonide (2mg/mL), i-budesonide (2mg/mL), i-mometasone (2mg/mL), i-fluticasone (2mg/mL), i-histamine hydrochloride (5mg/mL), i-alpha interferon (800IU/mL), i-zanamivir (20mg/mL), i-ribavirin (10mg/mL), i-oseltamivir (60ng/mL), i-peramivir (1mg/mL), i-lopinavir (500mg/mL), i-ritonavir(60mg/mL), i-mupirocin (20mg/mL), i-azithromycin (1mg/mL), i-ceftriaxone (40μg/mL), i-meropenem (200mg/mL), i-levofloxacin (10μg/mL) kunye ne-tobramycin (0.6mg/mL) yovavanyo lokuphazamiseka, kwaye iziphumo zibonisa ukuba izinto eziphazamisayo ezinomlinganiselo okhankanywe apha ngasentla azinazo iziphumo zokuphazamiseka kwiziphumo zovavanyo lwezifo ezibangela izifo. |
| Izixhobo Ezisebenzayo | Inkqubo ye-PCR ye-Applied Biosystems 7500 Real-TimeIinkqubo ze-PCR eziSebenzisiweyo ze-7500 ezikhawulezayo zeXesha langempela Iinkqubo ze-PCR zeQuantStudio®5 zeXesha langempela |
Ukuhamba komsebenzi
Ukhetho 1.
I-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) kunye ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006) eyenziwe yiJiangsu Macro & Micro-Test Med-Tech Co., Ltd. Umthamo wesampulu okhutshiweyo yi-200μL, kwaye umthamo wokucoca ocetyiswayo yi-80μL.
Ukhetho 2.
I-QIAamp Viral RNA Mini Kit (52904) eveliswe yi-QIAGEN okanye i-Nucleic Acid Extraction okanye i-Purification Kit (YDP315-R) eveliswe yi-Tiangen Biotech (Beijing) Co., Ltd. Umthamo wesampulu okhutshiweyo yi-140μL, kwaye umthamo wokucoca ocetyiswayo yi-60μL.
