I-Chlamydia Pneumoniae Nucleic Acid
Igama lemveliso
HWTS-RT023-Chlamydia Pneumoniae Nucleic Acid Detection Kit(Fluorescence PCR)
I-Epidemiology
Usulelo lwe-Acute breathing tract (ARTI) sisifo esixhaphakileyo kwizifo zabantwana, phakathi kwayo i-Chlamydia pneumoniae kunye ne-Mycoplasma pneumoniae usulelo ziibhaktheriya eziqhelekileyo ze-pathogenic kwaye zinosulelo oluthile, kwaye lunokudluliselwa ngendlela yokuphefumula ngamaconsi. Iimpawu zincinci, ubukhulu becala ngumqala obuhlungu, ukhohlokhohlo olomileyo, nomkhuhlane, kwaye abantwana bayo yonke iminyaka banokuchaphazeleka. Inani elikhulu leenkcukacha libonisa ukuba abantwana abaneminyaka yobudala besikolo abangaphezu kweminyaka eyi-8 kunye nabaselula liqela eliphambili elosulelwe yi-Chlamydia pneumoniae, elibalelwa malunga ne-10-20% ye-pneumonia efunyenwe kuluntu. Izigulana ezisele zikhulile ezine-immunity ephantsi okanye izifo ezingaphantsi nazo zisesichengeni sesi sifo. Kwiminyaka yakutshanje, izinga lokugula losulelo lwe-Chlamydia pneumoniae liye lanyuka unyaka nonyaka, kunye nesantya sosulelo phakathi kwabantwana besikolo sabasaqalayo kunye nabasesikolweni. Ngenxa yeempawu zokuqala ezingaqhelekanga kunye nexesha elide lokuqanduselwa kosulelo lweChlamydia pneumoniae, ukungaxilongwa kakuhle kunye namazinga oxilongo angaphoswanga aphezulu kuxilongo lweklinikhi, nto leyo eyalibazisa unyango lwabantwana.
Iiparamitha zobuGcisa
| Ugcino | ≤-18℃ |
| Beka ubomi kwishelufa | Iinyanga ezili-12 |
| Uhlobo loMfanekiso | isikhohlela, i-oropharyngeal swab |
| CV | ≤10.0% |
| LoD | 200Iikopi/mL |
| Ukuchaza ngokuthe ngqo | Iziphumo zovavanyo lwe-cross-reactivity zibonise ukuba akukho mpendulo emnqamlezweni phakathi kwale khithi kunye ne-Ureaplasma urealyticum, Mycoplasma genitalium, Mycoplasma hominis, Streptococcus pneumoniae, Mycoplasma pneumoniae, Haemophilus influenzae, Klebsiella pneumoniae, Staphylococcus aureus, Mycoccus aureus, Mycobacterium pnemogila I-Pseudomonas aeruginosa, i-Acinetobacter baumannii, intsholongwane yomkhuhlane A, intsholongwane ye-influenza B, uhlobo lwentsholongwane yeParainfluenza I/II/III/IV, Rhinovirus, Adenovirus, i-metapneumovirus yabantu, intsholongwane yokuphefumla ye-syncytial kunye ne-human genomic nucleic acids. |
| Izixhobo Ezisebenzayo | Iinkqubo ze-Biosystems ezisetyenzisiweyo 7500 Iinkqubo zePCR zeXesha langempela, Iinkqubo ze-Biosystems eziSetyenzisiweyo 7500 Iinkqubo zePCR ezikhawulezayo, QuantStudio®Iinkqubo zePCR ezi-5 zeXesha langempela, SLAN-96P Iinkqubo zePCR zeXesha lokwenyani (Hongshi Medical Technology Co., Ltd.), I-LightCycler®480 inkqubo yePCR yexesha lokwenyani, I-LineGene 9600 Plus Iinkqubo zokuFumana ze-PCR ngexesha langempela (i-FQD-96A, iteknoloji ye-Hangzhou Bioer), I-MA-6000 yeXesha lokwenyani iBhayisekile yoMyinge weThermal (iSuzhou Molarray Co., Ltd.), Inkqubo yePCR yeBioRad CFX96 yeXesha lokwenyani, BioRad CFX Opus 96 Real-Time PCR System. |
Ukuhamba komsebenzi
IMacro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (enokuthi isetyenziswe kunye neMacro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)), kunye neMacro & Micro-Test Viral DNA/RNA Kit (HWTS-17u-30)TM I-AIO800 (HWTS-EQ007)) yi-Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.
Umthamo wesampulu ekhutshiweyo yi-200μL kwaye umthamo we-elution ocetyiswayo yi-150μL.
