I-Chlamydia Pneumoniae Nucleic Acid
Igama lemveliso
Ikhithi yokuFumanisa i-HWTS-RT023-Chlamydia Pneumoniae Nucleic Acid (i-Fluorescence PCR)
I-Epidemiology
Usulelo olukhawulezileyo lwendlela yokuphefumla (ARTI) sisifo esixhaphakileyo kwiintsana zabantwana, apho phakathi kwazo iChlamydia pneumoniae kunye nosulelo lweMycoplasma pneumoniae ziibhaktheriya eziqhelekileyo ezibangela izifo kwaye zinosulelo oluthile, kwaye zinokudluliselwa ngendlela yokuphefumla ngamaconsi. Iimpawu zincinci, ikakhulu ngomphimbo obuhlungu, ukukhwehlela okomileyo, kunye nomkhuhlane, kwaye abantwana bayo yonke iminyaka basengozini. Inani elikhulu ledatha libonisa ukuba abantwana abakwiminyaka yesikolo abangaphezu kweminyaka esi-8 ubudala kunye nabantu abancinci liqela eliphambili elosulelwe yiChlamydia pneumoniae, elimalunga ne-10-20% yepneumonia efunyanwa luluntu. Izigulane ezindala ezine-immune system ephantsi okanye izifo ezingaphantsi nazo zisengozini yosulelo. Kwiminyaka yakutshanje, izinga lokugula losulelo lweChlamydia pneumoniae linyuke unyaka nonyaka, kunye nezinga losulelo phakathi kwabantwana abakwisikolo samabanga aphantsi kunye nabantwana abakwiminyaka yesikolo liphezulu. Ngenxa yeempawu zokuqala ezingaqhelekanga kunye nexesha elide lokufakwa kosulelo lweChlamydia pneumoniae, ukuxilongwa okungafanelekanga kunye namazinga okuchongwa aphosakeleyo aphezulu ekuchongweni kweklinikhi, ngaloo ndlela kulibazisa unyango lwabantwana.
Iiparameters zobugcisa
| Indawo yokugcina izinto | ≤-18℃ |
| Beka ubomi kwishelufa | Iinyanga ezili-12 |
| Uhlobo lweSibonelelo | isikhwehlela, i-oropharyngeal swab |
| CV | ≤10.0% |
| I-LoD | Iikopi ezingama-200/mL |
| Ukuchaneka | Iziphumo zovavanyo lwe-cross-reactivity zibonise ukuba akukho mpendulo idibeneyo phakathi kwale kit kunye ne-Ureaplasma urealyticum, iMycoplasma genitalium, iMycoplasma hominis, iStreptococcus pneumoniae, iMycoplasma pneumoniae, iHaemophilus influenzae, iKlebsiella pneumoniae, iStaphylococcus aureus, iMycobacterium tuberculosis, iLegionella pneumophila, iPseudomonas aeruginosa, iAcinetobacter baumannii, intsholongwane ye-influenza A, intsholongwane ye-influenza B, intsholongwane ye-Parainfluenza uhlobo lwe-I/II/III/IV, iRhinovirus, i-Adenovirus, i-human metapneumovirus, intsholongwane ye-respiratory syncytial kunye ne-human genomic nucleic acids. |
| Izixhobo Ezisebenzayo | Iinkqubo ze-PCR ze-Applied Biosystems 7500 Real-Time, Iinkqubo ze-PCR eziFakiweyo ze-7500 ze-Fast Real-Time PCR, I-QuantStudio®Iinkqubo ze-PCR ezi-5 zeXesha langempela, Iinkqubo ze-PCR ze-SLAN-96P zeXesha langempela (iHongshi Medical Technology Co., Ltd.), I-LightCycler®Inkqubo ye-PCR yeXesha langempela engama-480, Iinkqubo zokuFumanisa i-LineGene 9600 Plus Real-Time PCR (FQD-96A, iteknoloji ye-Hangzhou Bioer), I-MA-6000 Real-Time Quantitative Thermal Cycler (iSuzhou Molarray Co., Ltd.), Inkqubo ye-PCR ye-BioRad CFX96 yeXesha langempela, Inkqubo ye-PCR ye-BioRad CFX Opus 96 yeXesha langempela. |
Ukuhamba komsebenzi
I-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (engasetyenziswa kunye ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)), kunye ne-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-8) (engasetyenziswa kunye ne-EudemonTM I-AIO800 (HWTS-EQ007)) yiJiangsu Macro kunye neMicro-Test Med-Tech Co., Ltd.
Umthamo wesampulu ekhutshiweyo yi-200μL kwaye umthamo we-elution ocetyiswayo yi-150μL.
