Ikhithi yokuFumanisa iChlamydia Trachomatis Nucleic Acid (iFluorescence PCR)

Ikhithi yokuFumanisa iChlamydia Trachomatis Nucleic Acid (Fluorescence PCR) Umfanekiso obonisiweyo

Inkcazo emfutshane:

Le khithi isetyenziselwa ukufumanisa i-Chlamydia trachomatis nucleic acid kumchamo wamadoda, i-urethral swab yamadoda, kunye neesampuli ze-cervical swab yabasetyhini.

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Iinkcukacha zeMveliso

Iithegi zeMveliso

Igama lemveliso

Ikhithi yokuFumanisa iChlamydia Trachomatis Nucleic Acid (iFluorescence PCR)

Ukusetyenziswa Okucetywayo

Le khithi isetyenziselwa ukufumanisa i-Chlamydia trachomatis nucleic acid kumchamo wamadoda, i-urethral swab yamadoda, kunye neesampuli ze-cervical swab yabasetyhini.

I-Epidemiology

I-Chlamydia trachomatis (CT) luhlobo lwe-prokaryotic microorganism edla ngokuba yi-parasitic kwiiseli ze-eukaryotic. I-Chlamydia trachomatis yahlulwe yaba zii-serotypes ze-AK ngokwendlela ye-serotype. Usulelo lwe-Urogenital tract lubangelwa kakhulu yi-trachoma biological variant DK serotypes, kwaye amadoda abonakala kakhulu njenge-urethritis, enokupheliswa ngaphandle konyango, kodwa uninzi lwazo luhlala luhlala luhleli, luba mandundu ngamaxesha athile, kwaye lunokudityaniswa ne-epididymitis, proctitis, njl.njl. Amabhinqa anokubangelwa yi-urethritis, i-cervicitis, njl.njl., kunye neengxaki ezinzulu ze-salpingitis.

I-Epidemiology

FAM: Chlamydia trachomatis (CT)·

I-VIC(HEX): Ulawulo lwangaphakathi

Useto lweMiqathango yokwandisa i-PCR

Inyathelo	Iibhayisekile	Ubushushu	Ixesha	Qokelela Iimpawu zeFluorescent okanye Hayi
1	Umjikelo o-1	50℃	imizuzu emi-5	No
2	Umjikelo o-1	95℃	imizuzu eli-10	No
3	Iibhayisekile ezingama-40	95℃	Imizuzwana eli-15	No
4	Iibhayisekile ezingama-40	58℃	Imizuzwana engama-31	Ewe

Iiparameters zobugcisa

Indawo yokugcina izinto	≤-18℃ Ebumnyameni
Beka ubomi kwishelufa	Iinyanga ezili-12
Uhlobo lweSibonelelo	Ukuphuma komchamo kwamadoda, Ukuphuma komlomo wesibeleko kwabasetyhini, Umchamo wamadoda
Ct	≤38
CV	＜5.0%
I-LoD	Iikopi ezingama-400/mL
Ukuchaneka	Akukho sisombululo sidibeneyo sokufumanisa ezinye iintsholongwane ezinezifo ezithathelwana ngesondo ngale kit, ezifana neTreponema pallidum, iNeisseria gonorrhoeae, iUreaplasma urealyticum, iMycoplasma hominis, iMycoplasma genitalium, njl.njl., ezingaphandle koluhlu lokufumanisa lwekit.
Izixhobo Ezisebenzayo	Ingalingana nezixhobo zePCR ezikhanyayo ezithengiswayo. Iinkqubo ze-PCR ze-Applied Biosystems 7500 Real-Time Iinkqubo ze-PCR eziSebenzisiweyo ze-7500 ezikhawulezayo zeXesha langempela I-QuantStudio^®Iinkqubo ezi-5 ze-PCR zeXesha langempela Iinkqubo ze-PCR ze-SLAN-96P zeXesha langempela I-LightCycler^®Inkqubo ye-PCR yeXesha langempela ye-480 Inkqubo yokuHlola i-PCR yeLineGene 9600 Plus ngexesha langempela I-MA-6000 Real-Time Quantitative Thermal Cycler Inkqubo ye-PCR ye-BioRad CFX96 yeXesha langempela Inkqubo ye-PCR ye-BioRad CFX Opus 96 yeXesha langempela

Ukuhamba komsebenzi

Ikhithi yokuFumanisa iChlamydia Trachomatis Nucleic Acid (iFluorescence PCR)

Ii-reagents ziyafuneka kodwa azibonelelwa

I-saline eqhelekileyo, i-Macro kunye ne-Micro-Test Sample Collection, Mailing & Shipping Kit (HWTS-3030-1B), i-Macro kunye ne-Micro-Test Viral DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (engasetyenziswa kunye ne-Macro kunye ne-Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)), i-Nucleic Acid Extraction okanye i-Purification Reagent (YDP302) yiTiangen Biotech (Beijing) Co., Ltd.

Izinto ezisetyenziswayo ziyafuneka kodwa azibonelelwa

Iingcebiso ze-DNase/RNase ezingenazo, iiglavu ezilahlwayo, iityhubhu ze-EP ezingenazo i-DNase/RNase, iCentrifuge, iMagnetic stand, ii-strips ze-8-tube ze-qPCR esekelwe kwi-fluorescence.

Ngaphambili: Ikhithi yokuFumanisa i-Herpes Simplex Virus Type 2 Nucleic Acid (i-Fluorescence PCR)

Okulandelayo: Ikhithi yokuFumanisa iTrichomonas Vaginalis Nucleic Acid (iFluorescence PCR)

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