Chlamydia Trachomatis, Neisseria Gonorrhoeae kunye neTrichomonas vaginalis
Igama lemveliso
HWTS-UR041 Chlamydia Trachomatis, Neisseria Gonorrhoeae kunye neTrichomonas vaginalis iNucleic Acid Detection Kit (Fluorescence PCR)
I-Epidemiology
I-Chlamydia trachomatis (CT) luhlobo lwe-microorganism ye-prokaryotic ene-parasitic ngokungqongqo kwiiseli ze-eukaryotic.I-Chlamydia trachomatis yahlulwe ibe yi-AK serotypes ngokwendlela ye-serotype.Izifo ze-Urogenital zibangelwa kakhulu yi-trachoma biological variant DK serotypes, kwaye amadoda abonakaliswe kakhulu njenge-urethritis, enokuthi ikhululeke ngaphandle konyango, kodwa ininzi yabo ibe yinto engapheliyo, ikhula ngokukhawuleza, kwaye inokudibaniswa ne-epididymitis, i-proctitis, njl.
Umjelo
| FAM | I-Chlamydia trachomatis |
| ROX | Neisseria gonorrhoeae |
| CY5 | I-Trichomonal vaginitis |
| VIC/HEX | Ulawulo lwangaphakathi |
IiParameters zobuGcisa
| Ugcino | -18℃ |
| Beka ubomi kwishelufa | Iinyanga ezili-12 |
| Uhlobo loMfanekiso | Iswabhu yomlomo wesibeleko yabasetyhini,Iswabhu yelungu lobufazi,I-swab ye-urethral yamadoda |
| Ct | ≤38 |
| CV | <5% |
| LoD | 400Iikopi/mL |
| Ukuchaza ngokuthe ngqo | Akukho-cross-reactivity kunye nezinye iintsholongwane ze-STD zosulelo ngaphandle koluhlu lokukhangela ikiti yokuvavanya, njenge-Treponema pallidum, i-Mycoplasma hominis, i-Mycoplasma genitalium, i-Herpes simplex virus type 1, i-Herpes simplex virus type 2, i-Candida albicans, njl. |
| Izixhobo Ezisebenzayo | Isetyenziselwe i-Biosystems ye-7500 ye-Real-Time PCR System Iinkqubo ze-Biosystems eziSetyenzisiweyo 7500 Iinkqubo zePCR ezikhawulezayo QuantStudio®IiNkqubo ze-PCR ze-5 zeXesha langempela SLAN-96P Iinkqubo zePCR zeXesha langempela I-LightCycler®Inkqubo ye-PCR ye-480 yexesha langempela I-LineGene 9600 Plus Real-Time PCR Detection System MA-6000 Real-Time Quantitative Thermal Cycler I-BioRad CFX96 Inkqubo yePCR yexesha lokwenyani BioRad CFX Opus 96 Real-Time PCR System |
Ukuhamba komsebenzi
Ukhetho loku-1.
I-reagent yokutsalwa ecetyiswayo: I-Pipette 1mL yesampuli ukuba ivavanywe kwi-1.5mL ye-DNase / i-RNase-free centrifuge tube, i-centrifuge kwi-12000rpm imizuzu ye-3, ilahle i-supernatant kwaye ugcine i-precipitate.Yongeza i-200µL ye-saline eqhelekileyo kwimvula ukuze umise kwakhona.IMacro & Micro-Test General DNA/RNA Kit (HWTS-3019-50, HWTS-3019-32, HWTS-3019-48, HWTS-3019-96) (enokuthi isetyenziswe kunye neMacro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) nguJiangsu Macro & Micro-Test Med-Tech Co., Ltd. Ukukhutshwa kufuneka kwenziwe ngokomyalelo wokusetyenziswa.Umthamo wesampulu ekhutshiweyo yi-200µL, kwaye umthamo we-elution ocetyiswayo yi-80µL.
Ukhetho lwesi-2.
Isixhobo sokutsalwa esicetyiswayo: Ukutsalwa kwe-Nucleic Acid okanye iKit yokucoca (YDP302).Ukukhutshwa kufuneka kwenziwe ngokungqongqo ngokomyalelo wokusetyenziswa.Umthamo we-elution ocetyiswayo yi-80µL.
