I-Chlamydia Trachomatis, i-Neisseria Gonorrhoeae kunye ne-Trichomonas vaginalis
Igama lemveliso
I-HWTS-UR041 Chlamydia Trachomatis, Neisseria Gonorrhoeae kunye neTrichomonas vaginalis Nucleic Acid Detection Kit (Fluorescence PCR)
I-Epidemiology
I-Chlamydia trachomatis (CT) luhlobo lwe-prokaryotic microorganism edla ngokuba yi-parasitic kwiiseli ze-eukaryotic. I-Chlamydia trachomatis yahlulwe yaba zii-serotypes ze-AK ngokwendlela ye-serotype. Usulelo lwe-Urogenital tract lubangelwa kakhulu yi-trachoma biological variant DK serotypes, kwaye amadoda abonakala kakhulu njenge-urethritis, enokupheliswa ngaphandle konyango, kodwa uninzi lwawo luba nesifo esinganyangekiyo, siphindaphindeke, kwaye sinokudityaniswa ne-epididymitis, proctitis, njl.njl.
Itshaneli
| I-FAM | I-Chlamydia trachomatis |
| I-ROX | I-Neisseria gonorrhoeae |
| CY5 | I-Trichomonal vaginitis |
| I-VIC/HEX | Ulawulo lwangaphakathi |
Iiparameters zobugcisa
| Indawo yokugcina izinto | -18℃ |
| Beka ubomi kwishelufa | Iinyanga ezili-12 |
| Uhlobo lweSibonelelo | Iswabhu yomlomo wesibeleko yabasetyhini,Iswabhu yesini yabasetyhini,Iswabhu ye-urethra yamadoda |
| Ct | ≤38 |
| CV | <5% |
| I-LoD | 400Iikopi/mL |
| Ukuchaneka | Akukho kudibana kwezifo nezinye izifo ezibangelwa zizifo ezidluliselwa ... |
| Izixhobo Ezisebenzayo | Inkqubo ye-PCR ye-Applied Biosystems 7500 Real-Time Iinkqubo ze-PCR eziSebenzisiweyo ze-7500 ezikhawulezayo zeXesha langempela I-QuantStudio®Iinkqubo ezi-5 ze-PCR zeXesha langempela Iinkqubo ze-PCR ze-SLAN-96P zeXesha langempela I-LightCycler®Inkqubo ye-PCR yeXesha langempela ye-480 Inkqubo yokuHlola i-PCR yeLineGene 9600 Plus ngexesha langempela I-MA-6000 Real-Time Quantitative Thermal Cycler Inkqubo ye-PCR ye-BioRad CFX96 yeXesha langempela Inkqubo ye-PCR ye-BioRad CFX Opus 96 yeXesha langempela |
Ukuhamba komsebenzi
Ukhetho 1.
I-reagent yokukhupha ecetyiswayo: I-Pipette 1mL yesampulu eza kuvavanywa kwi-1.5mL yetyhubhu ye-DNase/RNase-free centrifuge, i-centrifuge kwi-12000rpm imizuzu emi-3, yilahle i-supernatant kwaye ugcine i-precipitate. Yongeza i-200µL ye-saline eqhelekileyo kwi-precipitate ukuze iphinde ixutywe. I-Macro & Micro-Test General DNA/RNA Kit (HWTS-3019-50, HWTS-3019-32, HWTS-3019-48, HWTS-3019-96) (engasetyenziswa ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) yiJiangsu Macro & Micro-Test Med-Tech Co., Ltd. Ukukhupha kufuneka kwenziwe ngokwemiyalelo yokusetyenziswa. Umthamo wesampulu okhutshiweyo yi-200µL, kwaye umthamo wokucoca ocetyiswayo yi-80µL.
Ukhetho 2.
Isixhobo sokukhupha esicetyiswayo: I-Nucleic Acid Extraction okanye i-Purification Kit (YDP302). Ukukhupha kufuneka kwenziwe ngokungqongqo ngokwemiyalelo yokusetyenziswa. Umthamo wokucoca ocetyiswayo yi-80µL.
