Ikhithi yokuFumanisa i-Herpes Simplex Virus Type 1 Nucleic Acid (Fluorescence PCR)
Igama lemveliso
Ikhithi yokuFumanisa i-Herpes Simplex Virus Type 1 Nucleic Acid (Fluorescence PCR)
I-Epidemiology
Izifo ezidluliselwa ngesondo (STDs) zisesesinye sezona zisongelo zibalulekileyo kukhuseleko lwempilo yoluntu kwihlabathi liphela, ezinokukhokelela ekungazali, ekuzaleni ngaphambi kwexesha, kwiithumba kunye neengxaki ezahlukeneyo ezinzulu.[3-6]. Kukho iintlobo ezininzi ze-STD pathogens, kuquka ibhaktheriya, iintsholongwane, i-chlamydia, i-mycoplasma kunye ne-spirochetes. Iintlobo eziqhelekileyo ziquka i-neisseria gonorrhoeae, i-mycoplasma genitalium, i-chlamydia trachomatis, i-herpes simplex virus type 1, i-herpes simplex virus type 2, i-mycoplasma hominis, ureaplasma urealyticum, njl.
Itshaneli
| I-FAM | Uhlobo 1 lwentsholongwane yeHerpes Simplex (HSV1) |
| I-ROX | Ulawulo lwangaphakathi |
Iiparameters zobugcisa
| Indawo yokugcina izinto | -18℃ |
| Beka ubomi kwishelufa | Iinyanga ezili-12 |
| Uhlobo lweSibonelelo | Iswabhu yomlomo wesibeleko yabasetyhini,Iswabhu ye-urethra yamadoda |
| Ct | ≤38 |
| CV | ≤5.0% |
| I-LoD | 500Iikopi/mL |
| Ukuchaneka | Vavanya ezinye iintsholongwane ze-STD, ezifana ne-treponema pallidum, i-chlamydia trachomatis, i-neisseria gonorrhoeae, i-mycoplasma hominis, i-mycoplasma genitalium, i-ureaplasma urealyticum, njl., akukho-cross-reactivity. |
| Izixhobo Ezisebenzayo | Iinkqubo ze-PCR ze-Applied Biosystems 7500 Real-Time Iinkqubo ze-PCR eziSebenzisiweyo ze-7500 ezikhawulezayo zeXesha langempela I-QuantStudio®Iinkqubo ezi-5 ze-PCR zeXesha langempela Iinkqubo ze-PCR ze-SLAN-96P zeXesha langempela I-LightCycler®Inkqubo ye-PCR yeXesha langempela ye-480 Inkqubo yokuHlola i-PCR yeLineGene 9600 Plus ngexesha langempela I-MA-6000 Real-Time Quantitative Thermal Cycler Inkqubo ye-PCR ye-BioRad CFX96 yeXesha langempela Inkqubo ye-PCR ye-BioRad CFX Opus 96 yeXesha langempela |
Ukuhamba komsebenzi
Ii-reagents ziyafuneka kodwa azibonelelwa
I-saline eqhelekileyo, i-Macro kunye ne-Micro-Test Sample Release Reagent (HWTS-3005-8), i-Macro kunye ne-Micro-Test General DNA/RNA Kit (HWTS-3017) kunye ne-Macro kunye ne-Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B), i-Nucleic Acid Extraction okanye i-Purification Reagent (YDP302) yiTiangen Biotech (Beijing) Co., Ltd.
Izinto ezisetyenziswayo ziyafuneka kodwa azibonelelwa
I-1.5mL yetyhubhu ye-centrifuge engena-DNase/RNase, i-DNase/RNase engena-Tip, i-ethanol epheleleyo, i-pipette, i-desktop centrifuge, umxube we-desktop ojikelezayo.
