MTHFR Gene Polymorphic Nucleic Acid

Inkcazelo emfutshane:

Le khithi isetyenziselwa ukufumanisa iindawo ezi-2 zokuguqula i-MTHFR gene.Ikhithi isebenzisa igazi elipheleleyo lomntu njengesampulu yokuvavanya ukubonelela ngovavanyo lomgangatho wemo yokuguquka.Inokunceda oogqirha ukuba bayile izicwangciso zonyango ezifanelekileyo kwiimpawu zomntu ngamnye ukusuka kwinqanaba le-molecular, ukuze kuqinisekiswe impilo yezigulana ngowona mlinganiselo mkhulu.


Iinkcukacha zeMveliso

Iithegi zeMveliso

Igama lemveliso

I-HWTS-GE004-MTHFR Gene Polymorphic Nucleic Acid Detection Kit (ARMS-PCR)

I-Epidemiology

I-Folic acid yivithamin enyibilikayo emanzini eyicofactor eyimfuneko kumendo womzimba.Kwiminyaka yakutshanje, inani elikhulu lezifundo ziqinisekisile ukuba, ukuguqulwa kwe-folate ye-metabolizing gene gene MTHFR kuya kukhokelela ekunqongophelweni kwe-folic acid emzimbeni, kwaye umonakalo oqhelekileyo wokunqongophala kwe-folic acid kubantu abadala unokubangela i-megaloblastic anemia, i-vascular. umonakalo we-endothelial, njl. Ukunqongophala kwe-Folic acid kubasetyhini abakhulelweyo abakwazi ukuhlangabezana neemfuno zabo kunye ne-fetus, enokubangela iziphene ze-neural tube, i-anencephaly, ukuzala, kunye nokuphuphuma kwesisu.Amanqanaba e-serum folate achatshazelwa yi-5,10-methylenetetrahydrofolate reductase (MTHFR) polymorphisms.Ukuguqulwa kwe-677C> T kunye ne-1298A>C kwi-MTHFR gene yenza ukuguqulwa kwe-alanine kwi-valine kunye ne-glutamic acid, ngokulandelanayo, kubangele ukunciphisa umsebenzi we-MTHFR kwaye ngenxa yoko kuncitshiswe ukusetyenziswa kwe-folic acid.

Umjelo

FAM I-MTHFR C677T
ROX I-MTHFR A1298C
VIC(HEX) Ulawulo lwangaphakathi

IiParameters zobuGcisa

Ugcino

≤-18℃

Beka ubomi kwishelufa

Iinyanga ezili-12

Uhlobo loMfanekiso

Igazi elisandul' ukuqokelelwa yi-EDTA anticoagulated

CV

≤5.0%

Ct

≤38

LoD

1.0ng/μL

Izixhobo Ezisebenzayo:

Iinkqubo ze-Biosystems ezisetyenzisiweyo 7500 Iinkqubo zePCR zeXesha langempela

Iinkqubo ze-Biosystems eziSetyenzisiweyo 7500 Iinkqubo zePCR ezikhawulezayo

I-SLAN ®-96P Iinkqubo ze-PCR zeXesha langempela

IQuantStudio™ 5 Iinkqubo zePCR zeXesha langempela

Inkqubo yePCR yeLightCycler®480 yeXesha langempela

I-LineGene 9600 Plus Real-Time PCR Detection System

MA-6000 Real-Time Quantitative Thermal Cycler

I-BioRad CFX96 Inkqubo yePCR yexesha lokwenyani

BioRad CFX Opus 96 Real-Time PCR System

Ukuhamba komsebenzi

Ukhetho loku-1

Ii-reagents zokutsalwa ezicetyiswayo: Macro & Micro-Test Genomic DNA Kit (HWTS-3014-32, HWTS-3014-48, HWTS-3014-96) kunye neMacro & Micro-Test Automatic Nucleic Acid Extractor(HWTS-3006C, HWTS-300) .

Ukhetho lwesi-2

Ii-reagents zokutsalwa ezicetyiswayo: I-Blood Genomic DNA Extraction Kit(YDP348, JCXB20210062) yi-Tiangen Biotech(Beijing) Co.,Ltd.I-Blood Genome Extraction Kit (A1120) yiPromega.


  • Ngaphambili:
  • Okulandelayo:

  • Bhala umyalezo wakho apha kwaye uwuthumele kuthi