I-Coxsackie Virus Type A16 Nucleic Acid

Le kit ithatha i-Enzymatic Probe Isothermal Amplification (EPIA), kwaye iyila i-primers ethile kunye ne-RNA equkethe i-probes (rProbe) yommandla ogcinwe kakhulu we-Cox A16, kwaye yongeza i-enzyme ye-Bst kunye ne-enzyme ye-RNaseH ngexesha elifanayo, apho i-left and Iziphelo zasekunene zesiseko se-RNA se-rProbe zibhalwe ngamaqela e-fluorescent kunye nesicimi, ngokulandelelanayo.Sebenzisa umsebenzi we-polymerase ye-DNA kunye ne-strand displacement ye-enzyme ye-Bst ukukhulisa ithagethi yokuvavanya kwiqondo lokushisa rhoqo, i-enzyme ye-RNaseH inokuqhawula iziseko ze-RNA kwi-target-probe hybrid chain, ukwenzela ukuba iqela le-fluorescent kunye ne-quencher ye-rProbe yahlulwe ngokwenza i-fluorescing.Ukongeza, iqhekeza lasekhohlo lesiseko se-rProbe RNA singasetyenziswa njenge-primer ukuqhubela phambili ukwandisa ukwenza imveliso, eqokelela ngakumbi imveliso.Isignali ye-fluorescent iqokelelwa ngokuqhubekayo kunye nokubunjwa kwemveliso, ngaloo ndlela iqonda ukufunyanwa kwe-nucleic acid ekujoliswe kuyo.