I-Coxsackie Virus Type A16 Nucleic Acid

Inkcazelo emfutshane:

Le khithi isetyenziselwa ukukhangela umgangatho we-in vitro we-Coxsackie virus uhlobo lwe-A16 nucleic acid kwi-swabs yomqala womntu.


Iinkcukacha zeMveliso

Iithegi zeMveliso

Igama lemveliso

HWTS-EV025-Coxsackie Virus Type A16 Nucleic Acid Detection Kit (Enzymatic Probe Isothermal Amplification)

Isatifikethi

CE

I-Epidemiology

Le kit ithatha i-Enzymatic Probe Isothermal Amplification (EPIA), kwaye iyila i-primers ethile kunye ne-RNA equkethe i-probes (rProbe) yommandla ogcinwe kakhulu we-Cox A16, kwaye yongeza i-enzyme ye-Bst kunye ne-enzyme ye-RNaseH ngexesha elifanayo, apho i-left and Iziphelo zasekunene zesiseko se-RNA se-rProbe zibhalwe ngamaqela e-fluorescent kunye nesicimi, ngokulandelelanayo.Sebenzisa umsebenzi we-polymerase ye-DNA kunye ne-strand displacement ye-enzyme ye-Bst ukukhulisa ithagethi yokuvavanya kwiqondo lokushisa rhoqo, i-enzyme ye-RNaseH inokuqhawula iziseko ze-RNA kwi-target-probe hybrid chain, ukwenzela ukuba iqela le-fluorescent kunye ne-quencher ye-rProbe yahlulwe ngokwenza i-fluorescing.Ukongeza, iqhekeza lasekhohlo lesiseko se-rProbe RNA singasetyenziswa njenge-primer ukuqhubela phambili ukwandisa ukwenza imveliso, eqokelela ngakumbi imveliso.Isignali ye-fluorescent iqokelelwa ngokuqhubekayo kunye nokubunjwa kwemveliso, ngaloo ndlela iqonda ukufunyanwa kwe-nucleic acid ekujoliswe kuyo.

Umjelo

FAM Uhlobo lwentsholongwane ye-Coxsackie A16
ROX ulawulo lwangaphakathi

IiParameters zobuGcisa

Ugcino

≤-18℃

Beka ubomi kwishelufa

Iinyanga ezili-12

Uhlobo loMfanekiso

Iiswabs zomqala ezisanda kuqokelela

CV

≤10.0%

Ct

≤38

LoD

2000Iikopi/mL

Izixhobo Ezisebenzayo

Iinkqubo ze-Biosystems ezisetyenzisiweyo 7500 Iinkqubo zePCR zeXesha langempelaSLAN-96P Iinkqubo zePCR zeXesha langempela

Inkqubo yePCR yeLightCycler®480 yeXesha langempela

Ixesha lokwenyani iFluorescence Constant Temperature System yokubona ubushushu obulula Amp HWTS1600

Ukuhamba komsebenzi

Ukhetho loku-1

I-reagent yokutsalwa ecetyiswayo: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3001, HWTS-3004-32, HWTS-3004-48) kunye neMacro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006)

Ukhetho lwesi-2

I-reagent yokutsalwa ecetyiswayo: I-Macro & Micro-Test Release Sample Release Reagent (HWTS-3005-8).


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