I-Chlamydia Trachomatis eyomiswe ngokuqandisa
Igama lemveliso
I-HWTS-UR032C/D-Ikhithi yokuFumanisa iChlamydia Trachomatis Nucleic Acid eyomileyo eqandisiweyo (i-Enzymatic Probe Isothermal Amplification)
I-Epidemiology
I-Chlamydia trachomatis (CT) luhlobo lwe-prokaryotic microorganism edla ngokuba yi-parasitic kwiiseli ze-eukaryotic.[1]I-Chlamydia trachomatis yahlulwe yaba zii-serotypes ze-AK ngokwendlela ye-serotype. Usulelo lwe-Urogenital tract lubangelwa kakhulu yi-trachoma biological variant DK serotypes, kwaye amadoda abonakala kakhulu njenge-urethritis, enokupheliswa ngaphandle konyango, kodwa uninzi lwazo luba nesifo esinganyangekiyo, siqhubeke sibi, kwaye sinokudityaniswa ne-epididymitis, proctitis, njl.njl.[2]Abafazi banokubangelwa yi-urethritis, i-cervicitis, njl.njl., kunye neengxaki ezinzulu ze-salpingitis.[3].
Itshaneli
| I-FAM | I-Chlamydia trachomatis (CT) |
| I-ROX | Ulawulo lwangaphakathi |
Iiparameters zobugcisa
| Indawo yokugcina izinto | ≤30℃ |
| Beka ubomi kwishelufa | Iinyanga ezili-12 |
| Uhlobo lweSibonelelo | Iswabhu yomlomo wesibeleko yabasetyhini Iswabhu ye-urethra yamadoda Umchamo wendoda |
| Tt | ≤28 |
| CV | ≤10.0% |
| I-LoD | Iikopi ezingama-400/mL |
| Ukuchaneka | Akukho kudibana phakathi kwale khithi kunye nezinye izifo ezibangelwa kusulelo lwendlela ye-genitourinary ezifana ne-Human papillomavirus type 16 enobungozi obukhulu, i-Human papillomavirus type 18, i-Herpes simplex virus type Ⅱ, i-Treponema pallidum, i-Ureaplasma urealyticum, i-Mycoplasma hominis, i-Mycoplasma genitalium, i-Staphylococcus epidermidis, i-Escherichia coli, i-Gardnerella vaginalis, i-Candida albicans, i-Trichomonas vaginalis, i-Lactobacillus crispatus, i-Adenovirus, i-Cytomegalovirus, i-Beta Streptococcus, i-Human immunodeficiency virus, i-Lactobacillus casei kunye ne-human genomic DNA, njl. |
| Izixhobo Ezisebenzayo | Inkqubo ye-PCR ye-Applied Biosystems 7500 Real-Time Iinkqubo ze-PCR eziSebenzisiweyo ze-7500 ezikhawulezayo zeXesha langempela I-QuantStudio®Iinkqubo ezi-5 ze-PCR zeXesha langempela Iinkqubo ze-PCR ze-SLAN-96P zeXesha langempela (iHongshi Medical Technology Co., Ltd.) I-LightCycler®Inkqubo ye-PCR yeXesha langempela ye-480 Inkqubo yokuFumanisa i-LineGene 9600 Plus yeXesha langempela le-PCR (i-FQD-96A, iteknoloji ye-Hangzhou Bioer) I-MA-6000 Real-Time Quantitative Thermal Cycler Inkqubo ye-PCR ye-BioRad CFX96 Real-Time kunye neNkqubo ye-PCR ye-BioRad CFX Opus 96 Real-Time Inkqubo yokuFumanisa i-Fluorescence Isothermal ye-Easy Amp Real-time(IsiNgesi)I-HWTS-1600）. |
Ukuhamba komsebenzi
Ukhetho 1.
I-Macro & Micro-Test Sample Release Reagent (HWTS-3005-8). Ukukhupha kufuneka kwenziwe ngokuhambelana ne-IFU. Yongeza i-DNA yesampulu ekhutshwe yi-sample release release reagent kwi-reaction buffer kwaye uyivavanye ngqo kwisixhobo, okanye iisampulu ezikhutshiweyo mazigcinwe kwi-2-8℃ kungabi ngaphezu kweeyure ezingama-24.
Ukhetho 2.
Ikhithi ye-DNA/RNA Jikelele ye-Macro kunye ne-Micro-Test (HWTS-301)7-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) kunye neMacro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B). Ukukhupha kufuneka kwenziwe ngokuhambelana ne-IFU, kwaye umthamo wokucoca ocetyiswayo yi-80μL. I-DNA yesampulu ekhutshwe ngendlela ye-magnetic bead ifudunyezwa kwi-95°C imizuzu emi-3 ize ihlanjwe ngoko nangoko imizuzu emi-2. Yongeza i-DNA yesampulu ecutshungulweyo kwi-reaction buffer kwaye uvavanyo kwisixhobo okanye kwiisampulu ezicutshungulweyo kufuneka zigcinwe ngaphantsi kwe-18°C kangangeenyanga ezi-4. Inani lokuqandisa nokunyibilikisa okuphindaphindiweyo akufuneki lidlule imijikelo emi-4.
