IChlamydia Trachomatis eyomiswe ngumkhenkce
Igama lemveliso
HWTS-UR032C/D-I-Chlamydia Trachomatis yeNucleic Acid eyomiswe ngumkhenkce (i-Enzymatic Probe Isothermal Amplification)
I-Epidemiology
I-Chlamydia trachomatis (CT) luhlobo lwe-microorganism ye-prokaryotic ene-parasitic ngokungqongqo kwiiseli ze-eukaryotic.[1].I-Chlamydia trachomatis yahlulwe ibe yi-AK serotypes ngokwendlela ye-serotype.Usulelo lwe-Urogenital tract lubangelwa kakhulu yi-trachoma biological variant DK serotypes, kwaye amadoda abonakaliswe kakhulu njenge-urethritis, enokukhululwa ngaphandle konyango, kodwa ininzi yabo ibe yinto engapheliyo, ngamaxesha athile, kwaye inokudibaniswa ne-epididymitis, i-proctitis, njl.[2].Abasetyhini banokubangelwa yi-urethritis, i-cervicitis, njl., kunye neengxaki ezinzulu ze-salpingitis.[3].
Umjelo
| FAM | I-Chlamydia trachomatis (CT) |
| ROX | Ulawulo lwangaphakathi |
IiParameters zobuGcisa
| Ugcino | ≤30℃ |
| Beka ubomi kwishelufa | Iinyanga ezili-12 |
| Uhlobo loMfanekiso | Iswabhu yomlomo wesibeleko yabasetyhini I-swab ye-urethral yamadoda Umchamo wendoda |
| Tt | ≤28 |
| CV | ≤10.0% |
| LoD | Iikopi ezingama-400/mL |
| Ukuchaza ngokuthe ngqo | akukho kuphinda kusebenze phakathi kwale khithi kunye nezinye iintsholongwane ze-genitourinary pathogens ezifana nomngcipheko ophezulu we-Human papillomavirus type 16, Human papillomavirus type 18, Herpes simplex virus type Ⅱ, Treponema pallidum, Ureaplasma urealyticum, Mycoplasma hominis, Mycoplasmspilogenider , Escherichia coli, Gardnerella vaginalis, Candida albicans, Trichomonas vaginalis, Lactobacillus crispatus, Adenovirus, Cytomegalovirus, Beta Streptococcus, Human immunodeficiency virus, Lactobacillus casei kunye ne-DNA ye-genomic yabantu, njl. |
| Izixhobo Ezisebenzayo | Isetyenziselwe i-Biosystems ye-7500 ye-Real-Time PCR System Iinkqubo ze-Biosystems eziSetyenzisiweyo 7500 Iinkqubo zePCR ezikhawulezayo QuantStudio®IiNkqubo ze-PCR ze-5 zeXesha langempela SLAN-96P Iinkqubo zePCR zeXesha lokwenyani(Hongshi Medical Technology Co., Ltd.) I-LightCycler®Inkqubo ye-PCR ye-480 yexesha langempela I-LineGene 9600 Plus ye-Real-Time PCR Detection System (FQD-96A, iteknoloji ye-Hangzhou Bioer) MA-6000 Real-Time Quantitative Thermal Cycler Inkqubo ye-BioRad CFX96 yeXesha langempela lePCR kunye neBioRad CFX Opus 96 Inkqubo yePCR yexesha lokwenyani Easy Amp Real-time Fluorescence Isothermal Ukufunyanwa System(HWTS-1600）. |
Ukuhamba komsebenzi
Ukhetho loku-1.
I-Macro kunye ne-Micro-Test yokuKhupha iSampuli ye-Reagent (HWTS-3005-8).Ukukhutshwa kufuneka kuqhutywe ngokuhambelana ne-IFU.Yongeza isampuli ye-DNA ekhutshwe yi-reagent yokukhululwa kwesampulu kwi-reaction buffer kunye nokuvavanya kwisixhobo ngokuthe ngqo, okanye iisampuli ezikhutshiweyo kufuneka zigcinwe kwi-2-8 ℃ ngaphandle kweeyure ezingama-24.
Ukhetho lwesi-2.
IMacro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) kunye ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B).Ukutsalwa kufuneka kuqhutywe ngokuhambelana ngqongqo ne-IFU, kwaye umthamo we-lution ocetyiswayo yi-80μL.Isampuli ye-DNA ekhutshwe yi-magnetic bead method ifudunyezwa kwi-95 ° C imizuzu emi-3 kwaye ngoko nangoko i-ice-bathed imizuzu emi-2.Yongeza isampula ye-DNA esetyenzisiweyo kwi-reaction buffer kunye novavanyo kwisixhobo okanye iisampulu ezicutshungulweyo kufuneka zigcinwe ngaphantsi kwe -18 ° C ngaphandle kweenyanga ezi-4.Inani lokukhenkceza okuphindaphindiweyo kunye nokunyibilika akufanele kudlule imijikelo emi-4.
