IChlamydia Trachomatis eyomiswe ngumkhenkce
Igama lemveliso
HWTS-UR032C/D-I-Chlamydia Trachomatis yeNucleic Acid eyomiswe ngumkhenkce (i-Enzymatic Probe Isothermal Amplification)
I-Epidemiology
I-Chlamydia trachomatis (CT) luhlobo lwe-microorganism ye-prokaryotic ene-parasitic ngokungqongqo kwiiseli ze-eukaryotic.[1]. I-Chlamydia trachomatis yahlulwe ibe yi-AK serotypes ngokwendlela ye-serotype. Usulelo lwe-Urogenital tract lubangelwa kakhulu yi-trachoma biological variant DK serotypes, kwaye amadoda abonakaliswe kakhulu njenge-urethritis, enokukhululwa ngaphandle konyango, kodwa ininzi yabo ibe yinto engapheliyo, ngamaxesha athile, kwaye inokudibaniswa ne-epididymitis, i-proctitis, njl.[2]. Abasetyhini banokubangelwa yi-urethritis, i-cervicitis, njl., kunye neengxaki ezinzulu ze-salpingitis.[3].
Umjelo
| FAM | I-Chlamydia trachomatis (CT) |
| ROX | Ulawulo lwangaphakathi |
Iiparamitha zobuGcisa
| Ugcino | ≤30℃ |
| Beka ubomi kwishelufa | Iinyanga ezili-12 |
| Uhlobo loMfanekiso | Iswabhu yomlomo wesibeleko yabasetyhini I-swab ye-urethral yamadoda Umchamo wendoda |
| Tt | ≤28 |
| CV | ≤10.0% |
| LoD | Iikopi ezingama-400/mL |
| Ukuchaza ngokuthe ngqo | Akukho kuphinda kusebenze phakathi kwale khithi kunye nezinye iintsholongwane ze-genitourinary tract pathogens ezifana nomngcipheko ophezulu we-Human papillomavirus type 16, Human papillomavirus type 18, Herpes simplex virus type Ⅱ, Treponema pallidum, Ureaplasma urealyticum, Mycoplasma hominis, Mycoplasma, Statistics Escherichia coli, Gardnerella vaginalis, Candida albicans, Trichomonas vaginalis, Lactobacillus crispatus, Adenovirus, Cytomegalovirus, Beta Streptococcus, Human immunodeficiency virus, Lactobacillus casei kunye neDNA ye-genomic yomntu, njl. |
| Izixhobo Ezisebenzayo | Isetyenziselwe i-Biosystems ye-7500 ye-Real-Time PCR System Iinkqubo ze-Biosystems eziSetyenzisiweyo 7500 Iinkqubo zePCR ezikhawulezayo QuantStudio®IiNkqubo ze-PCR ze-5 zeXesha langempela SLAN-96P Iinkqubo zePCR zeXesha lokwenyani(Hongshi Medical Technology Co., Ltd.) I-LightCycler®Inkqubo ye-PCR ye-480 yexesha langempela I-LineGene 9600 Plus ye-Real-Time PCR Detection System (FQD-96A, iteknoloji ye-Hangzhou Bioer) MA-6000 Real-Time Quantitative Thermal Cycler Inkqubo ye-BioRad CFX96 yeXesha langempela lePCR kunye neBioRad CFX Opus 96 Inkqubo yePCR yexesha lokwenyani Easy Amp Real-time Fluorescence Isothermal Ukufunyanwa System(HWTS-1600）. |
Ukuhamba komsebenzi
Ukhetho loku-1.
I-Macro kunye ne-Micro-Test yokuKhupha iSampule ye-Reagent (HWTS-3005-8). Ukukhutshwa kufuneka kuqhutywe ngokungqongqo ngokuhambelana ne-IFU. Yongeza isampuli ye-DNA ekhutshwe yi-reagent yokukhululwa kwesampulu kwi-reagent buffer kunye nokuvavanya kwisixhobo ngokuthe ngqo, okanye iisampuli ezikhutshiweyo kufuneka zigcinwe kwi-2-8 ℃ ngaphandle kweeyure ezingama-24.
Ukhetho lwesi-2.
IMacro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) kunye ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B). Ukutsalwa kufuneka kuqhutywe ngokuhambelana ngqongqo ne-IFU, kwaye umthamo we-lution ocetyiswayo yi-80μL. Isampuli ye-DNA ekhutshwe yi-magnetic bead method ifudunyezwa kwi-95 ° C imizuzu emi-3 kwaye ngoko nangoko i-ice-bathed imizuzu emi-2. Yongeza isampula ye-DNA esetyenzisiweyo kwi-reaction buffer kunye novavanyo kwisixhobo okanye iisampulu ezicutshungulwayo kufuneka zigcinwe ngaphantsi kwe-18 ° C ngaphandle kweenyanga ezi-4. Inani lokukhenkceza okuphindaphindiweyo kunye nokunyibilika akufanele kudlule imijikelo emi-4.
