Iintsholongwane Ezintandathu Zokuphefumla Eziyomisiweyo Eziqandisiweyo

Iintsholongwane Ezintandathu Zokuphefumla Ezomileyo Eziqandisiweyo Umfanekiso Obonakalayo

Inkcazo emfutshane:

Le mveliso isetyenziselwa ukufumanisa intsholongwane ye-respiratory syncytial virus (RSV) kwi-in vitro, i-adenovirus (Adv), i-human metapneumovirus (hMPV), i-rhinovirus (Rhv), i-parainfluenza virus type I/II/III (PIVI/II/III) kunye ne-Mycoplasma pneumoniae (MP) nucleic acids kwiisampuli ze-human nasopharyngeal swab.

Thumela i-imeyile kuthi

Iinkcukacha zeMveliso

Iithegi zeMveliso

Igama lemveliso

I-HWTS-RT192-Isixhobo sokuFumanisa iiPathogens zeNucleic Acid eziNqandisiweyo eziNqandisiweyo ezintandathu (i-Fluorescence PCR)

I-Epidemiology

Usulelo lwendlela yokuphefumla lolona hlobo luqhelekileyo lwesifo ebantwini, olunokwenzeka nakweyiphi na isini, ubudala kunye nommandla, kwaye lolunye lwezona zizathu zibalulekileyo zokugula nokufa emhlabeni [1]. Iintsholongwane zokuphefumla eziqhelekileyo ngokwezonyango ziquka intsholongwane yokuphefumla ehambelanayo, i-adenovirus, i-human metapneumovirus, i-rhinovirus, intsholongwane ye-parainfluenza (I/II/III) kunye ne-mycoplasma pneumoniae, njl. [2,3]. Iimpawu zeklinikhi kunye neempawu ezibangelwa lusulelo lwendlela yokuphefumla ziyafana, kodwa usulelo olubangelwa ziintsholongwane ezahlukeneyo luneendlela ezahlukeneyo zonyango, iziphumo zonyango kunye nendlela yesifo [4,5]. Okwangoku, iindlela eziphambili zokufumanisa iintsholongwane zokuphefumla kwilabhoratri ziquka: ukwahlulwa kwentsholongwane, ukufunyanwa kwe-antigen kunye nokufunyanwa kwe-nucleic acid, njl. Le khithi ifumanisa kwaye ichonge ii-nucleic acids ezithile zentsholongwane kubantu abaneempawu kunye neempawu zosulelo lokuphefumla, kunye nezinye iziphumo zeklinikhi nezelabhoratri ukunceda ekuchongweni kosulelo lwentsholongwane yokuphefumla.

Iiparameters zobugcisa

Indawo yokugcina izinto	2-28℃
Beka ubomi kwishelufa	Iinyanga ezili-12
Uhlobo lweSibonelelo	I-nasopharyngeal swab
Ct	RSV,Adv,hMPV,Rhv,PIV,MP Ct≤35
I-LoD	Iikopi ezingama-200/mL
Ukuchaneka	Ukusabela okunqamlezileyo: Akukho kusabela okunqamlezileyo phakathi kwekhithi kunye nentsholongwane yeBoca, iCytomegalovirus, intsholongwane ye-Epstein-Barr, intsholongwane yeHerpes simplex, intsholongwane ye-varicella zoster, intsholongwane yeMumps, i-Enterovirus, intsholongwane yemasisi, intsholongwane ye-human coronavirus, iSARS Coronavirus, iMERS Coronavirus, iRotavirus, iNorovirus, iChlamydia pneumoniae, iStreptococcus pneumoniae, iKlebsiella pneumoniae, iStreptococcus pyogenes, iLegionella, iPneumospora, iHaemophilus influenzae, iBacillus pertussis, iStaphylococcus aureus, iMycobacterium tuberculosis, igonococcus, iCandida albicans, iCandida glabra, iAspergillus fumigatus, iCryptococcus neoformans, iStreptococcus salivarius, iMoraxella catarrh, iLactobacillus, iCorynebacterium, iDNA ye-human genomic.
Izixhobo Ezisebenzayo	Isebenza kwi-reagent yovavanyo lwe-Type I: Iinkqubo ze-PCR ze-Applied Biosystems 7500 Real-Time, Iinkqubo ze-PCR ze-SLAN-96P zeXesha langempela (iHongshi Medical Technology Co., Ltd.). Isebenza kwi-reagent yovavanyo lwe-Type II: U-Eudemon^TMI-AIO800 (HWTS-EQ007) yiJiangsu Macro kunye neMicro-Test Med-Tech Co., Ltd.

Ukuhamba komsebenzi

I-PCR eqhelekileyo

I-Macro & Micro-Test General DNA/RNA Kit (HWTS-3019) (engasetyenziswa kunye ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, (HWTS-3006B) yiJiangsu Macro & Micro-Test Med-Tech Co., Ltd. ziyacetyiswa xa kutsalwa isampuli kwaye amanyathelo alandelayo kufuneka enziwe ngokuhambelana ne-IFU yeKit.