I-Zaire eyomiswe ngumkhenkce kunye ne-Sudan Ebolavirus Nucleic Acid
Igama lemveliso
I-HWTS-FE035-eyomileyo iZaire kunye neSudan Ebolavirus Nucleic Acid Detection Kit (Fluorescence PCR)
I-Epidemiology
I-Ebolavirus yeye-Filoviridae, eyintsholongwane engahlulwanga enomsonto omnye we-RNA. Iintsholongwane ziyimicu emide ene-avareji yobude be-virion obuyi-1000nm kunye nobubanzi obumalunga ne-100nm. I-genome ye-Ebolavirus yi-RNA engagatywanga-negative-strand enobungakanani be-18.9kb, i-encoding 7 iiprotheyini zesakhiwo kunye ne-1 yeprotheni engeyiyo yesakhiwo. I-Ebolavirus inokwahlulwa ibe ziindidi ezifana neZaire, Sudan, Bundibugyo, Tai Forest kunye neReston. Phakathi kwazo, uhlobo lweZaire kunye nohlobo lwaseSudan kuye kwaxelwa ukuba kubangele ukubhubha kwabantu abaninzi ngenxa yosulelo. I-EHF (i-Ebola Hemorrhagic Fever) sisifo esosulelayo esopha kakhulu esibangelwa yi-Ebolavirus. Abantu bosulelwa ikakhulu ngokudibana nolwelo lomzimba, ukukhuselwa kunye nelindle lezigulane okanye izilwanyana ezosulelekileyo, kwaye ukubonakaliswa kweklinikhi ikakhulu kuphuma umkhuhlane, ukopha kunye nokonakala kwamalungu amaninzi. I-EHF inezinga eliphezulu lokufa kwe-50% -90%. Okwangoku, iindlela zokuxilongwa kwe-Ebolavirus ziziimvavanyo zaselabhorethri, kubandakanywa iinkalo ezimbini: ukufumanisa i-etiological kunye nokufumanisa i-serological. Ukufunyanwa kwe-Etiological kubandakanya ukufumanisa ii-antigens zentsholongwane kwiisampuli zegazi nge-ELISA, ukufumanisa i-nucleic acids ngeendlela zokukhulisa njenge-RT-PCR, njl. Ukufunyanwa kwe-serological kubandakanya ukufumanisa i-Serum ethile ye-IgM antibodies ngokubamba i-ELISA, kunye nokufumanisa i-Serum ethile ye-IgG antibodies ngu-ELISA, i-immunofluorescence, njl.
Iiparamitha zobuGcisa
Ugcino | ≤30℃ |
Beka ubomi kwishelufa | Iinyanga ezili-12 |
Uhlobo loMfanekiso | serum, iplaiisampulu sma |
CV | ≤5.0% |
LoD | Iikopi ezingama-500/μL |
Izixhobo Ezisebenzayo | Isebenza ukuchwetheza i-reagent yokubona: Iinkqubo ze-Biosystems ezisetyenzisiweyo 7500 Iinkqubo zePCR zeXesha langempela, QuantStudio®Iinkqubo zePCR ezi-5 zeXesha langempela, SLAN-96P Iinkqubo zePCR zeXesha lokwenyani (Hongshi Medical Technology Co., Ltd.), I-LineGene 9600 Plus Iinkqubo zokuFumana ze-PCR ngexesha langempela (i-FQD-96A, iteknoloji ye-Hangzhou Bioer), I-MA-6000 yeXesha lokwenyani iBhayisekile yoMyinge weThermal (iSuzhou Molarray Co., Ltd.), I-BioRad CFX96 Inkqubo ye-PCR yexesha langempela, i-BioRad CFX Opus 96 Inkqubo ye-PCR yexesha lokwenene. Isebenza kuhlobo lwe-II yokufumanisa i-reagent: EudemonTMI-AIO800 (HWTS-EQ007) nguJiangsu Macro kunye neMicro-Test Med-Tech Co., Ltd. |
Ukuhamba komsebenzi
IMacro & Micro-Test Viral DNA/RNA Kit (HWTS-3001, HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) kunye neMacro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006). Ukukhutshwa kufuneka kuqhutywe ngokwemiyalelo, kwaye umthamo wesampulu ekhutshiweyo ngu-200μL kunye nomthamo we-elution ocetyiswayo ngu-80μL.