Hepatitis intsholongwane
Igama lemveliso
I-HWTS-HP005 hepatitis Intsholongwane ye-vidi ye-acid hid (fluorescence pcr)
Isipikumu
I-Hepatitis Intsholongwane (i-HAV) yeyona nto iphambili ye-hepatitis yentsholongwane. Intsholongwane yintsholongwane eqingqiweyo ye-RNA kwaye yeyokwaya kwiHepadnavirus yencwadi yentsapho iPictornavididae. I-Hepatititi intsholongwane, ikakhulu idluliselwa yindlela ye-Fecal-Omlomo, ixhaphake kubushushu, i-acids, kunye nezinyibilikisi, kwaye zinokuphila ixesha elide kwi-Shellfish, amanzi, emhlabeni, okanye i-1-3]. Idluliselwa ngokuba nokutya okungcolisekileyo okanye amanzi, okanye ngokuthe ngqo kuwo umntu ngamnye. Ukutya okunxulunyaniswa ne-HAV kubandakanya i-oysters kunye ne-clams, i-trewberries, i-raspberries, i-blueberries, imihla enamagqabi, kunye netamatisi eyomileyo [4-6].
Ijelo
| Indlala | I-hav nucleic acid |
| Rox | Ulawulo lwangaphakathi |
Iiparamitha zobugcisa
| Ukugcinwa | ≤-18 ℃ |
| Beka ubomi kwishelufa | Ulwelo: Iinyanga ezili-9, uLyophilbezer: iinyanga ezili-12 |
| Uhlobo locaphu | I-serum / isitulo |
| Tt | ≤38 |
| CV | ≤5.0% |
| Indawo yokuhlala | Iikopi ezi-2 / μl |
| Ubuchule | Sebenzisa i-kits ukuvavanya ezinye iintsholongwane zehetitis ezifana neHepatitis B, C, D, E, E-Enterovin-Barring, i-EDERAGINE, intsholongwane kaGawulayo. |
| Izixhobo ezifanelekileyo | Faka isicelo se-biosystslates 700 ye-PCR ye-PCR ye-PCR Faka isicelo se-biosystemsts ezingama-700 ngokukhawuleza kwe-PCR ye-PCR Ubungakanani®Iinkqubo ezi-5 zokwenyani ze-PCR I-Slan-96p yangempela ye-PCR ye-PCR (Hongshi yonyango lweTekhnoloji Co., Ltd.) I-FIMCYCLER ®480 ye-PALCR ye-PCR ye-PCR I-Linegene 9600 kunye neNkqubo yokuKhangela ye-PFRR ye-PFR (FQD-96a, i-Hangzhou Bioer), i-MA-6000 yexesha lokwenyani I-biorad cfx96 yeyokwenyani ye-PFRR I-biorad cfx opi 96 Inkqubo ye-PFR ye-PCR |
Ukuhamba komsebenzi
Iisampulu ze-serum
Inketho 1.
I-Macro kunye ne-RNA yeDNA / RNA Kit (HWTS-3017-50, HWTS-3017-7-53, HWTS-3017-7-7), HWTS-30006C, I-HWTS-3006B). Kufuneka ikhutshwe ngokwemiyalelo. Ivolumu yesiqhelo yesiqhelo yi-80μl.
Inketho 2.
I-TIAMAMP ye-tiamp virus DNA / RNA Kit (YDP35-R) yenziwe nguTiangen Biotech (iBeijing) Co., i-LTD. Ifanele ikhutshwe ngokwemiyalelo. Ivolumu yesampulu ekhutshiweyo yi-140μl. Ivolumu yesiqhelo yesiqhelo yi-60μl.
2.Iisampulu zesitulo
I-Macro kunye ne-RNA yeDNA / RNA Kit (HWTS-3017-50, HWTS-3017-7-53, HWTS-3017-7-7), HWTS-30006C, I-HWTS-3006B). Kufuneka ikhutshwe ngokwemiyalelo. Ivolumu yesiqhelo yesiqhelo yi-80μl.
