Intsholongwane yeHepatitis A
Igama lemveliso
Ikhithi yokuFumanisa i-HWTS-HP005 Hepatitis A Virus Nucleic Acid (i-Fluorescence PCR)
I-Epidemiology
Intsholongwane yeHepatitis A (HAV) yeyona nto ibangela i-hepatitis ebangelwa yintsholongwane ebukhali. Le ntsholongwane yintsholongwane ye-RNA ene-single-sense kwaye ikwi-genus yeHepadnavirus yosapho lwePicornaviridae. Intsholongwane yeHepatitis A, edluliselwa kakhulu yindlela yokungena emlonyeni, imelana nobushushu, ii-asidi, kunye nezinye izinto ezininzi eziphilayo, inokuphila ixesha elide kwi-shellfish, emanzini, emhlabeni, okanye kwi-sediments esecaleni kolwandle [1-3]. Idluliselwa ngokutya okanye amanzi angcolileyo, okanye ngokuthe ngqo idluliselwa ukusuka komnye umntu ukuya komnye. Ukutya okunxulunyaniswa ne-HAV kuquka ii-oyster kunye nee-clams, ii-strawberry, ii-raspberries, ii-blueberries, ii-dates, imifuno eluhlaza, kunye neetamatisi ezomileyo kancinci [4‒6].
Itshaneli
| I-FAM | I-HAV nucleic acid |
| I-ROX | Ulawulo lwangaphakathi |
Iiparameters zobugcisa
| Indawo yokugcina izinto | ≤-18℃ |
| Beka ubomi kwishelufa | Ulwelo: Iinyanga ezili-9, I-Lyophilized: Iinyanga ezili-12 |
| Uhlobo lweSibonelelo | I-serum/isitulo |
| Tt | ≤38 |
| CV | ≤5.0% |
| I-LoD | Iikopi ezi-2/μL |
| Ukuchaneka | Sebenzisa ezi zixhobo ukuvavanya ezinye iintsholongwane ze-hepatitis ezifana ne-hepatitis B, C, D, E, enterovirus 71, i-coxsackie virus, i-Epstein-Barr virus, i-norovirus, i-HIV kunye ne-human genome. Akukho ntshukumo idibeneyo. |
| Izixhobo Ezisebenzayo | Inkqubo ye-PCR ye-Applied Biosystems 7500 Real-Time Iinkqubo ze-PCR eziSebenzisiweyo ze-7500 ezikhawulezayo zeXesha langempela I-QuantStudio®Iinkqubo ezi-5 ze-PCR zeXesha langempela Iinkqubo ze-PCR ze-SLAN-96P zeXesha langempela (iHongshi Medical Technology Co., Ltd.) Inkqubo ye-PCR yeLightCycler®480 yeXesha langempela Inkqubo yokuFumanisa i-LineGene 9600 Plus Real-Time PCR (FQD-96A, iteknoloji ye-Hangzhou Bioer), i-MA-6000 Real-Time Quantitative Thermal Cycler Inkqubo ye-PCR ye-BioRad CFX96 yeXesha langempela Inkqubo ye-PCR ye-BioRad CFX Opus 96 yeXesha langempela |
Ukuhamba komsebenzi
Iisampuli zeSerum
Ukhetho 1.
I-Macro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) kunye ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B). Ifanele ikhutshwe ngokwemiyalelo. Umthamo wokucoca ocetyiswayo yi-80µL.
Ukhetho 2.
I-TIANamp Virus DNA/RNA Kit (YDP315-R) eyenziwe yiTiangen Biotech (Beijing) Co., Ltd. Ifanele ikhutshwe ngokwemiyalelo. Umthamo wesampulu okhutshiweyo yi-140μL. Umthamo wokucoca ocetyiswayo yi-60μL.
2.Iisampulu zeSitulo
I-Macro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) kunye ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B). Ifanele ikhutshwe ngokwemiyalelo. Umthamo wokucoca ocetyiswayo yi-80µL.
